Integrin beta-1/CD29 Protein, Human (HEK293, His)
Based on 1 Customer Validation
Integrin beta-1/CD29 forms distinct heterodimers that act as multifunctional receptors for extracellular matrix proteins. It recognizes collagen, fibronectin, fibrinogen and laminin and plays a key role in cell functions such as migration, adhesion and angiogenesis. Integrin beta-1/CD29 Protein, Human (708a.a, HEK293, His) is the recombinant human-derived Integrin beta-1/CD29 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
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Biological Activity
Description
Integrin beta-1/CD29 forms distinct heterodimers that act as multifunctional receptors for extracellular matrix proteins. It recognizes collagen, fibronectin, fibrinogen and laminin and plays a key role in cell functions such as migration, adhesion and angiogenesis. Integrin beta-1/CD29 Protein, Human (708a.a, HEK293, His) is the recombinant human-derived Integrin beta-1/CD29 protein, expressed by HEK293 , with C-His labeled tag.
Background
The Integrin beta-1/CD29 protein, forming heterodimers with various alpha subunits, serves as a versatile receptor for a multitude of extracellular matrix proteins. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1, and alpha-11/beta-1 recognize collagen, while Integrins alpha-2/beta-2, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1, and alpha-V/beta-1 act as receptors for fibronectin. Additionally, Integrin alpha-5/beta-1 serves as a receptor for fibrinogen, and Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1, and alpha-7/beta-1 are receptors for laminin. Specific integrins, such as alpha-4/beta-1 and alpha-9/beta-1, play roles in recognizing VCAM1 and other ligands, contributing to diverse cellular functions including migration, adhesion, and angiogenesis. Beta-1 integrins, recognizing the R-G-D sequence, are implicated in various physiological processes, such as osteoblast compaction, cell motility, and cytokinesis completion. Furthermore, Integrin beta-1/CD29 plays a key role in facilitating cell invasion, matrix degradation, and myoblast differentiation, highlighting its significance in cellular adhesion, signaling, and developmental processes.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized human Fibronectin, at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD29 protein. The ED50 for this effect is 306.6 ng/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P05556-1 (Q21-D728)
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Molecular Construction
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N-term
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ITGB1 (Q21-D728)
Accession # P05556-1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ITGB1; Very Late Activation Protein, Beta Polypeptide; Prev. MSK12; Integrin VLA-4 Beta Subunit; Prev. FNRB; VLA-4 Subunit Beta; Prev. MDF2; Integrin Beta 1; GPIIA; Integrin Beta; Integrin Beta-1; CD29 Antigen; CD29; VLA-BETA; Integrin, Beta 1 (Fibronecti
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AA Sequence
QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD
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Molecular Weight
Approximately 100-130 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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