Klotho beta Protein, Human (HEK293, His)
Based on 1 Customer Validation
Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Klotho beta protein plays a critical role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme controlling bile acid synthesis. Although its glycosidase activity may be inactive, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, promoting their interaction. Klotho beta Protein, Human (HEK293, His) is the recombinant human-derived Klotho beta protein, expressed by HEK293 , with C-His labeled tag.
The Klotho beta protein plays a significant role in transcriptional regulation by contributing to the repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. While likely inactive as a glycosidase, Klotho beta enhances the binding ability of FGFR1 and FGFR4 to FGF21, indicating its involvement in fibroblast growth factor (FGF) signaling. The protein directly interacts with both FGF19 and FGF21, underscoring its association with crucial components of the FGF pathway. These interactions, particularly with FGF21, suggest a potential role in metabolic regulation and highlight Klotho beta's involvement in modulating cellular responses and metabolic pathways (
1.Immobilized Human Beta Klotho at 2 μg/mL (100 μL/Well). Dose response curve for Anti-Beta Klotho Antibody1 with the EC50 is < 80.2 ng/mL determined by ELISA.
2.Immobilized Human Beta Klotho at 2 μg/mL (100μl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of < 89.8 ng/ml determined by ELISA.
3.Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2198 ng/mL in the presence of 1μg/mL Human FGF-21, corresponding to a specific activity is 4.549×103 units/mg.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q86Z14 (M30-T983)
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Molecular Construction
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N-term
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Klotho beta (M30-T983)
Accession # Q86Z14 -
His
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C-term
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Protein Length
Partial
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Synonyms
KLB; B-Klotho; Klotho Beta; BKL; Beta-Klotho; Klotho Beta Like; BetaKlotho; KLB Protein; Klotho Beta-Like Protein
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AA Sequence
MSNGGLQRSVILSALILLRAVTGFSGDGRAIWSKNPNFTPVNESQLFLYDTFPKNFFWGIGTGALQVEGSWKKDGKGPSIWDHFIHTHLKNVSSTNGSSDSYIFLEKDLSALDFIGVSFYQFSISWPRLFPDGIVTVANAKGLQYYSTLLDALVLRNIEPIVTLYHWDLPLALQEKYGGWKNDTIIDIFNDYATYCFQMFGDRVKYWITIHNPYLVAWHGYGTGMHAPGEKGNLAAVYTVGHNLIKAHSKVWHNYNTHFRPHQKGWLSITLGSHWIEPNRSENTMDIFKCQQSMVSVLGWFANPIHGDGDYPEGMRKKLFSVLPIFSEAEKHEMRGTADFFAFSFGPNNFKPLNTMAKMGQNVSLNLREALNWIKLEYNNPRILIAENGWFTDSRVKTEDTTAIYMMKNFLSQVLQAIRLDEIRVFGYTAWSLLDGFEWQDAYTIRRGLFYVDFNSKQKERKPKSSAHYYKQIIRENGFSLKESTPDVQGQFPCDFSWGVTESVLKPESVASSPQFSDPHLYVWNATGNRLLHRVEGVRLKTRPAQCTDFVNIKKQLEMLARMKVTHYRFALDWASVLPTGNLSAVNRQALRYYRCVVSEGLKLGISAMVTLYYPTHAHLGLPEPLLHADGWLNPSTAEAFQAYAGLCFQELGDLVKLWITINEPNRLSDIYNRSGNDTYGAAHNLLVAHALAWRLYDRQFRPSQRGAVSLSLHADWAEPANPYADSHWRAAERFLQFEIAWFAEPLFKTGDYPAAMREYIASKHRRGLSSSALPRLTEAERRLLKGTVDFCALNHFTTRFVMHEQLAGSRYDSDRDIQFLQDITRLSSPTRLAVIPWGVRKLLRWVRRNYGDMDIYITASGIDDQALEDDRLRKYYLGKYLQEVLKAYLIDKVRIKGYYAFKLAEEKSKPRFGFFTSDFKAKSSIQFYNKVISSRGFPFENSSSRCSQTQENT
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Molecular Weight
Approximately 115-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (242 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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