LCAT Protein, Human (HEK293, His)

LCAT protein is synthesized in the liver and plays a vital role in the metabolism of plasma lipoproteins by converting cholesterol and phosphatidylcholine in HDL and LDL. LCAT Protein, Human (HEK293, His) is the recombinant human-derived LCAT protein, expressed by HEK293 , with C-6*His labeled tag.

LCAT, a pivotal enzyme in the extracellular metabolism of plasma lipoproteins, is primarily synthesized in the liver and secreted into the plasma. In this crucial role, LCAT converts cholesterol and phosphatidylcholines (lecithins) on the surface of both high and low-density lipoproteins (HDLs and LDLs) to cholesteryl esters and lysophosphatidylcholines. The resulting cholesteryl esters are transported back to the liver. LCAT exhibits a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Beyond its hepatic functions, LCAT is produced in the brain by primary astrocytes, where it esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia, influencing cerebral spinal fluid (CSF) APOE- and APOA1 levels. In collaboration with APOE and the cholesterol transporter ABCA1, LCAT plays a pivotal role in the maturation of glial-derived, nascent lipoproteins. Additionally, LCAT is essential for remodeling high-density lipoprotein particles into their spherical forms and catalyzes the hydrolysis of platelet-activating factor (PAF) to lyso-PAF, as well as the esterification of (24S)-hydroxycholesterol (24(S)OH-C), known as cerebrosterol, to produce 24(S)OH-C monoesters.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P04180 (F25-E440)

3931  [NCBI]

Full Length of Mature Protein

FWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE

Approximately 66 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.