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Nectin-1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Nectin-1 is critical in cell adhesion and synaptogenesis, promotes neurite outgrowth, and serves as a receptor for alphaherpesviruses, including HSV-1, HSV-2, and pseudorabies virus. It promotes viral entry and is involved in homodimerization, trans-heterodimerization with NECTIN3, and interactions with FGFR, AFDN, and HSV glycoprotein D. Nectin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Nectin-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Mouse (HEK293, His)
  • Nectin-1 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

Nectin-1 is critical in cell adhesion and synaptogenesis, promotes neurite outgrowth, and serves as a receptor for alphaherpesviruses, including HSV-1, HSV-2, and pseudorabies virus. It promotes viral entry and is involved in homodimerization, trans-heterodimerization with NECTIN3, and interactions with FGFR, AFDN, and HSV glycoprotein D. Nectin-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Nectin-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Nectin-1, a pivotal player in cell adhesion and synaptogenesis, exhibits neurite outgrowth-promoting activity, underscoring its importance in neurodevelopmental processes. Functioning as a receptor for alphaherpesviruses, including HSV-1, HSV-2, and pseudorabies virus, Nectin-1 facilitates viral entry into cells. The protein engages in both cis- and trans-homodimerization and can also form trans-heterodimers with NECTIN3, initiating the formation of puncta adherentia junctions crucial for axon-dendrite interactions. Additionally, its interaction with FGFR1, FGFR2, and FGFR3, as well as AFDN, establishes connections with the actin cytoskeleton, contributing to its diverse cellular roles. Notably, Nectin-1 interacts with HSV glycoprotein D (gD), highlighting its involvement in viral infection processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1. The ED50 for this effect is 151 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1.The ED50 for this effect is 151 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-His
  • Accession

    Q9JKF6 (Q31-A354)

  • Gene ID
    58235  [NCBI]
  • Molecular Construction
    • N-term
    • Nectin-1 (Q31-A354)
      Accession # Q9JKF6
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    NECTIN1; OFC7; Prev. PVRL1; Poliovirus Receptor-Related 1 (Herpesvirus Entry Mediator C); Prev. HVEC; Herpesvirus Ig-Like Receptor; Prev. ED4; Ectodermal Dysplasia 4 (Margarita Island Type); Nectin-1; Herpes Simplex Virus Type 1 Sensitivity; PRR1; Herpesv

  • AA Sequence

    QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKASNGSKQNMAIYNPTMGVSVLPPYEKRVEFLRPSFIDGTIRLSGLELEDEGMYICEFATFPTGNRESQLNLTVMAKPTNWIEGTRAVLRARKGQDDKVLVATCTSANGKPPSAVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHRQSLACIVNYHLDRFRESLTLNVQYEPEVTIEGFDGNWYLQRTDVKLTCKADANPPATEYHWTTLNGSLPKGVEAQNRTLFFRGPITYSLAGTYICEATNPIGTRSGQVEVNITEFPYTPTPEHGRRAGQMPTA

  • Molecular Weight

    Approximately 50-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Human Nectin-3 is present at 1 μg/mL, can bind Recombinant Mouse Nectin-1.The ED50 for this effect is 151 ng/mL.

  • Nectin-1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Nectin-1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Nectin-1 Protein, Mouse (HEK293, His)
  • Nectin-1 Protein, Mouse (HEK293, His) Product Image