NGFR Protein, Rabbit (HEK293, hFc)
Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells. NGFR Protein, Rabbit (HEK293, hFc) is a recombinant protein with a C-Terminal Fc label, It consists of 242 amino acids (M1-D242) and is produced in HEK293 cells.
- Species: Rabbit
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Nerve Growth Factor Receptor (NGFR) also known as CD271, p75NTR, TNFRSF16, is a transmembrane low-affinity receptor for the neurotrophin family. NGFR has no intrinsic tyrosine kinase activity. NGFR can act as a tumour suppressor, negatively regulating cell growth and proliferation[1]. NGF-NGFR cascade activates NF-κB, leading to inhibition of apoptosis, increases survival of schwannoma, and breast cancer cells[3]. NGFR Protein, Rabbit (HEK293, hFc) is a recombinant protein with a C-Terminal Fc label, It consists of 242 amino acids (M1-D242) and is produced in HEK293 cells.
Background
Nerve Growth Factor Receptor (NGFR) is expressed not only in nervous tissue, but also in non-neuronal normal and cancer cells, such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells and myoepithelial cells[1].
Human NGFR shares 92.45% aa sequence identity with mouse NGFR protein and 92.42% aa sequence identity with rat NGFR protein.
Nerve Growth Factor Receptor (NGFR) is a type-I transmembrane protein, a typical structure of the TNFR superfamily and devoid of intrinsic catalytic activity. NGFR signaling involves activation of NF-kB (Rel/NF-kB transcription factors) and the phosphorylation of the transcription factor c-Jun kinase (JNK), as well as increased production of ceramide, leading to gene transcription or programmed cell death[2].
NGFR induces p53-dependent apoptosis and cell growth arrest as well as suppressed tumor growth[3]. The low-affinity nerve growth factor receptor (NGFR) p75NGFR induces apoptosis in the absence of nerve growth factor (NGF) binding but enhances neural survival when bound by NGF. NGFR enhances beta-amyloid peptide toxicity[4]. NGFR signal can induce the subsequent downregulation of melanoma antigens and eventually suppress CTL activation[5].
Technical Parameters
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Species Rabbit
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Source HEK293
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Tag C-hFc
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Accession
XP_008269543.1 (K22-D242)
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Molecular Construction
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N-term
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NGFR (K22-D242)
Accession # XP_008269543.1 -
hFc
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C-term
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Protein Length
Partial
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Synonyms
NGFR; Low Affinity Neurotrophin Receptor P75NTR; Nerve Growth Factor Receptor; TNFR Superfamily, Member 16; TNFRSF16; NGF Receptor; P75NTR; Gp80-LNGFR; CD271; P75 ICD; Tumor Necrosis Factor Receptor Superfamily Member 16; Nerve Growth Factor Receptor (TNF
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AA Sequence
KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDTVCRCAYGYYQDETTGRCEAYRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECSRWADAECEAIPGRWTTRSPAPEGSDSMAPSTQVPEGPPEQELPASTAADVVTTVMGSSQPVATRGTAD
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Predicted Molecular Mass
50.1 kDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
References
[1]. Reis-Filho JS, et al. Distribution and significance of nerve growth factor receptor (NGFR/p75NTR) in normal, benign and malignant breast tissue. Mod Pathol. 2006 Feb;19(2):307-19. [Content Brief]
[2]. Micera A, et al. Nerve growth factor and tissue repair remodeling: trkA(NGFR) and p75(NTR), two receptors one fate. Cytokine Growth Factor Rev. 2007 Jun-Aug;18(3-4):245-56. [Content Brief]
[3]. Zhou X, et al. Nerve growth factor receptor negates the tumor suppressor p53 as a feedback regulator. Elife. 2016 Jun 10;5:e15099. [Content Brief]
[4]. Rabizadeh S, et al. Expression of the low-affinity nerve growth factor receptor enhances beta-amyloid peptide toxicity. Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10703-6. [Content Brief]
[5]. Furuta J, et al. CD271 on melanoma cell is an IFN-γ-inducible immunosuppressive factor that mediates downregulation of melanoma antigens. J Invest Dermatol. 2014 May;134(5):1369-1377. [Content Brief]
[6]. Chung MK, et al. CD271 Confers an Invasive and Metastatic Phenotype of Head and Neck Squamous Cell Carcinoma through the Upregulation of Slug. Clin Cancer Res. 2018 Feb 1;24(3):674-683. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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