OTUB1 Protein, Human (His)

OTUB1 protein directly interacts with RNF128, forming a ternary complex with USP8 through OTUB1's binding to the C-terminal UCH catalytic domain of USP8. OTUB1 Protein, Human (His) is the recombinant human-derived OTUB1 protein, expressed by E. coli , with N-His labeled tag. The total length of OTUB1 Protein, Human (His) is 271 a.a., with molecular weight of ~34-37 kDa.

OTUB1 protein forms a direct interaction with RNF128, and together, they constitute a ternary complex involving USP8. The ternary complex is established through the interaction of OTUB1 with the C-terminal UCH catalytic domain of USP8.

The specific activity was determined to be 72.755 pmol/min/ml in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.

Human

E. coli

N-His

Q96FW1-1 (M1-K271)

55611  [NCBI]

MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK

Approximately 34-37 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, 20% Glycerol, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.