OTUB1 Protein, Human (His)
Based on 1 Customer Validation
OTUB1 protein directly interacts with RNF128, forming a ternary complex with USP8 through OTUB1's binding to the C-terminal UCH catalytic domain of USP8. OTUB1 Protein, Human (His) is the recombinant human-derived OTUB1 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
OTUB1 protein directly interacts with RNF128, forming a ternary complex with USP8 through OTUB1's binding to the C-terminal UCH catalytic domain of USP8. OTUB1 Protein, Human (His) is the recombinant human-derived OTUB1 protein, expressed by E. coli , with N-His labeled tag.
Background
OTUB1 protein forms a direct interaction with RNF128, and together, they constitute a ternary complex involving USP8. The ternary complex is established through the interaction of OTUB1 with the C-terminal UCH catalytic domain of USP8.
Verified Bioactivity
The specific activity was determined to be >30 pmol/min/mL in a DUB assay using recombinant human ubiquitin-based proluciferin substrate.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-10*His
-
Accession
Q96FW1-1 (M1-K271)
-
Molecular Construction
-
N-term
-
10*His
-
OTUB1 (M1-K271)
Accession # Q96FW1-1 -
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
OTUB1; FLJ40710; OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1; OTB1; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 1; OTU1; Ubiquitin-Specific-Processing Protease OTUB1; Ubiquitin-Specific Protease Otubain 1; Deubiquitinating Enzyme OTUB1;
-
AA Sequence
MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK
-
Predicted Molecular Mass
32.7 kDa
-
Molecular Weight
Approximately 37 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (236 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
