OTUB2 Protein, Human (His)
Based on 1 Customer Validation
OTUB2 is a hydrolase that efficiently removes conjugated ubiquitin from proteins in vitro, implying a crucial role in preventing protein degradation and regulating turnover. Its deubiquitinating activity targets “Lys-11”, “Lys-48” and “Lys-63” linked polyubiquitin chains, with preference for the latter. OTUB2 Protein, Human (His) is the recombinant human-derived OTUB2 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
OTUB2 is a hydrolase that efficiently removes conjugated ubiquitin from proteins in vitro, implying a crucial role in preventing protein degradation and regulating turnover. Its deubiquitinating activity targets “Lys-11”, “Lys-48” and “Lys-63” linked polyubiquitin chains, with preference for the latter. OTUB2 Protein, Human (His) is the recombinant human-derived OTUB2 protein, expressed by E. coli , with N-6*His labeled tag.
As a hydrolase, OTUB2 exhibits the capacity to efficiently remove conjugated ubiquitin from proteins in vitro, suggesting a pivotal regulatory role in protein turnover by preventing degradation. With its deubiquitination activity, OTUB2 acts on 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitin chains, displaying a preference for the latter. This specificity underscores OTUB2's selectivity in modulating diverse ubiquitin linkages, emphasizing its potential significance in orchestrating cellular processes related to protein ubiquitination and degradation.
The specific activity was determined to be 73.477 nmol/min/mg in a DUB assay.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
Q96DC9-1 (M1-H234)
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Molecular Construction
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N-term
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6*His
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OTUB2 (M1-H234)
Accession # Q96DC9-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
OTUB2; Otubain-2; Prev. C14orf137; MGC3102; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 2; OTB2; Ubiquitin-Specific-Processing Protease OTUB2; OTU2; OTU Domain, Ubiquitin Aldehyde Binding 2; Ubiquitin-Specific Protease Otubain 2; Deubiquitina
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AA Sequence
MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
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Molecular Weight
Approximately 30 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, 500 mM arginine, pH 10.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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