OTUB2 Protein, Human (His)

OTUB2 is a hydrolase that efficiently removes conjugated ubiquitin from proteins in vitro, implying a crucial role in preventing protein degradation and regulating turnover. Its deubiquitinating activity targets “Lys-11”, “Lys-48” and “Lys-63” linked polyubiquitin chains, with preference for the latter. OTUB2 Protein, Human (His) is the recombinant human-derived OTUB2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of OTUB2 Protein, Human (His) is 234 a.a., with molecular weight of ~28 kDa.

As a hydrolase, OTUB2 exhibits the capacity to efficiently remove conjugated ubiquitin from proteins in vitro, suggesting a pivotal regulatory role in protein turnover by preventing degradation. With its deubiquitination activity, OTUB2 acts on 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitin chains, displaying a preference for the latter. This specificity underscores OTUB2's selectivity in modulating diverse ubiquitin linkages, emphasizing its potential significance in orchestrating cellular processes related to protein ubiquitination and degradation.

The specific activity was determined to be 73.477 nmol/min/mg in a DUB assay.

Human

E. coli

N-6*His

Q96DC9 (M1-H234)

78990  [NCBI]

MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH

Approximately 28 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 500 mM arginine, pH 10.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.