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P2Y1 Protein, Human (sf9, FLAG)

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The P2Y1 protein acts as a receptor for extracellular adenine nucleotides, specifically ADP. In platelets, ADP binding activates phospholipase C, leading to intracellular calcium mobilization, platelet shape changes, and aggregation. P2Y1 Protein, Human (sf9, FLAG) is the recombinant human-derived P2Y1 protein, expressed by Sf9 insect cells, with C-10*His and N-Flag labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The P2Y1 protein acts as a receptor for extracellular adenine nucleotides, specifically ADP. In platelets, ADP binding activates phospholipase C, leading to intracellular calcium mobilization, platelet shape changes, and aggregation. P2Y1 Protein, Human (sf9, FLAG) is the recombinant human-derived P2Y1 protein, expressed by Sf9 insect cells, with C-10*His and N-Flag labeled tag.

Background

The P2Y1 receptor serves as a receptor for extracellular adenine nucleotides, particularly ADP. Upon binding to ADP, this receptor, found notably in platelets, triggers the mobilization of intracellular calcium ions through the activation of phospholipase C. Consequently, this activation induces changes in platelet shape and, ultimately, platelet aggregation. ATP acts as an antagonist, inhibiting the ADP-induced mobilization of calcium ions. Specific antagonists of the P2Y1 receptor, such as A3P5PS, A3P5P, and A2P5P, have been identified and shown to impede downstream signaling events mediated by the mobilization of calcium ions from intracellular stores, as well as platelet shape changes in response to extracellular ADP.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-10*His;N-Flag
  • Accession

    P47900 (T2-K247&M1-E54 of Rubredoxin&P253-L373, D320N)

  • Gene ID
    5028
  • Molecular Construction
    • N-term
    • Flag
    • P2Y1 (T2-K247&M1-E54 of Rubredoxin&P253-L373, D320N)
      Accession # P47900
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    P2RY1; P2Y Purinoceptor 1; Purinergic Receptor P2Y1; ADP Receptor; P2Y1; P2 Purinoceptor Subtype Y1; SARCC; Platelet ADP Receptor; Suppressing Androgen Receptor In Renal Cell Carcinoma; Purinergic Receptor; Purinergic Receptor P2Y, G-Protein Coupled, 1; A

  • AA Sequence

    DYKDDDDKTEVLWPAVPNGTDAAFLAGPGSSWGNSTVASTAAVSSSFKCALTKTGFQFYYLPAVYILVFIIGFLGNSVAIWMFVFHMKPWSGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPLKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYKMKKYTCTVCGYIYNPEDGDPDNGVNPGTDFKDIPDDWVCPLCGVGKDQFEEVEEPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVNPILYFLAGDTFRRRLSRATRKASRRSEANLQSKSEDMTLNILPEFKQNGDTSLLEVLFQGPHHHHHHHHHH

  • Predicted Molecular Mass

    50.7 kDa

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM HEPES pH 7.5, 150 mM NaCl, 0.01%, w/v LMNG, 0.001%, w/v CHS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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