PGK1 Protein, Mouse (sf9, His)

The PGK1 protein catalyzes the conversion of 1,3-bisphosphoglycerate into 3-phosphoglycerate and plays a central role in ATP production.It also acts as a polymerase alpha cofactor protein and may be involved in primer recognition.PGK1 Protein, Mouse (sf9, His) is the recombinant mouse-derived PGK1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

PGK1 protein, a pivotal contributor to cellular energy dynamics, orchestrates a fundamental step in the glycolytic pathway by catalyzing the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, thereby playing a central role in ATP production. Beyond its established glycolytic function, PGK1 exhibits versatility as it appears to act as a polymerase alpha cofactor protein, potentially involved in primer recognition processes. This dual functionality highlights PGK1's significance beyond its canonical role in glycolysis. Furthermore, there is an intriguing suggestion that PGK1 may be implicated in sperm motility, hinting at its potential involvement in reproductive processes. In essence, PGK1 emerges as a multifaceted protein, influencing both energy metabolism and cellular functions, thus contributing to the intricacies of cellular physiology.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

Sf9 insect cells

C-His

P09411 (M1-V417)

18655  [NCBI]

Full Length

MSLSNKLTLDKLDVKGKRVVMRVDFNVPMKNNQITNNQRIKAAVPSIKFCLDNGAKSVVLMSHLGRPDGVPMPDKYSLEPVAAELKSLLGKDVLFLKDCVGPEVENACANPAAGTVILLENLRFHVEEEGKGKDASGNKVKAEPAKIDAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPQKAGGFLMKKELNYFAKALESPERPFLAILGGAKVADKIQLINNMLDKVNEMIIGGGMAFTFLKVLNNMEIGTSLYDEEGAKIVKDLMSKAEKNGVKITLPVDFVTADKFDENAKTGQATVASGIPAGWMGLDCGTESSKKYAEAVGRAKQIVWNGPVGVFEWEAFARGTKSLMDEVVKATSRGCITIIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKVLPGVDALSNV

Approximately 43-46 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.