PIP4K2A Protein, Human (HEK293, His)

The PIP4K2A protein phosphorylates phosphatidylinositol 5-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP4K2A Protein, Human (HEK293, His) is the recombinant human-derived PIP4K2A protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PIP4K2A Protein, Human (HEK293, His) is 406 a.a., with molecular weight of 52-58 kDa.

The PIP4K2A protein plays a multifaceted role in cellular regulation, catalyzing the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) at the fourth hydroxyl of the myo-inositol ring to generate phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). This protein exhibits both ATP- and GTP-dependent kinase activities, contributing to its versatile enzymatic repertoire, and may modulate cytosolic PtdIns5P levels in response to tyrosine phosphorylation activation. Additionally, PIP4K2A is essential for lysosome-peroxisome membrane contacts, influencing intracellular cholesterol transport by regulating peroxisomal PtdIns(4,5)P2 levels. In collaboration with PIP4K2B, it plays a role in mediating autophagy during nutrient stress, impacting autophagosome-lysosome fusion and cellular lipid metabolism. PIP4K2A's involvement in thrombopoiesis and megakaryocyte maturation, coupled with its negative regulation of insulin signaling through a catalytic-independent mechanism, further underscores its diverse functions in cellular processes. Interactions with PIP5Ks highlight its role in suppressing PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3, revealing its intricate regulatory connections in cellular signaling pathways.

Human

HEK293

C-6*His

P48426 (M1-T406)

5305  [NCBI]

MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDDFKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARSGARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIVTRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLKKDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSPPLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNPEQYSKRFLDFIGHILT

52-58 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.