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PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi)

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PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

PLAU or urokinase-type plasminogen activator (uPA) critically initiates fibrinolysis by cleaving plasminogen to form active plasmin.This enzymatic conversion is critical for regulating clot dissolution and tissue remodeling.PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived PLAU/uPA protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

Background

PLAU, also known as urokinase-type plasminogen activator (uPA), plays a crucial role in fibrinolysis by specifically cleaving the zymogen plasminogen to generate the active enzyme plasmin. This enzymatic conversion is a pivotal step in the regulation of blood clot dissolution and tissue remodeling. PLAU's ability to activate plasmin sets in motion a cascade of proteolytic events, contributing to the breakdown of fibrin clots and extracellular matrix components. This process is essential for various physiological functions, including wound healing, tissue repair, and the resolution of blood clots.

Verified Bioactivity

Human uPAR, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 8.05 μM as determined in SPR assay (Biacore T200).

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-Avi;C-8*His
  • Accession

    P06869 (G21-F433)

  • Gene ID
    18792  [NCBI]
  • Molecular Construction
    • N-term
    • PLAU (G21-F433)
      Accession # P06869
    • 8*His-Avi
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Conjugation

    Biotin

  • Conjugate Method

    The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

  • Synonyms

    PLAU; Plasminogen Activator, Urinary; Plasminogen Activator, Urokinase; PLAU Protein; UPA; BDPLT5; Urokinase-Type Plasminogen Activator; U-PA; URK; ATF; U-Plasminogen Activator; QPD

  • AA Sequence

    GSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF

  • Predicted Molecular Mass

    49 kDa

  • Molecular Weight

    Approximately 30-35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi) Product Image
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  • PLAU/uPA Protein, Mouse (Biotinylated, HEK293, His-Avi) Product Image