PRKAR1A Protein, Mouse (sf9, His)

PRKAR1A protein is a regulatory subunit of cAMP-dependent protein kinase that mediates cellular responses to cAMP signaling. The inactive holoenzyme has two regulatory chains and two catalytic chains. PRKAR1A Protein, Mouse (sf9, His) is the recombinant mouse-derived PRKAR1A protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of PRKAR1A Protein, Mouse (sf9, His) is 381 a.a., with molecular weight of ~44.6 kDa.

PRKAR1A protein serves as a regulatory subunit in the cAMP-dependent protein kinases, playing a pivotal role in mediating cellular responses to cAMP signaling. The inactive holoenzyme comprises two regulatory chains and two catalytic chains. Upon activation by cAMP, the regulatory dimer dissociates, releasing two active catalytic monomers. PRKAR1A interacts with PRKACA and PRKACB, forming a dynamic complex involved in cAMP-dependent signaling cascades. Notably, it associates with RFC2, suggesting a potential role in cell survival mechanisms. Additional interactions include AKAP4, facilitating intricate signaling events, and RARA, where the interaction, influenced by cAMP or FSH, regulates RARA transcriptional activity. PRKAR1A further engages with phosphorylated PJA2, PRKX, CBFA2T3, and smAKAP, contributing to the modulation of cAMP-dependent protein kinase functions and targeting PRKAR1A to specific cellular locations, such as the plasma membrane. Additionally, PRKAR1A exhibits interactions with AICDA, indicating a role in diverse cellular processes. These interactions underscore the multifaceted involvement of PRKAR1A in cellular signaling and regulation.

Mouse

Sf9 insect cells

C-His

Q9DBC7 (M1-V381)

19084  [NCBI]

Approximately 44.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.