PRKG2 Protein, Human (Active, sf9, GST)
PRKG2 protein is a key regulator that activates CFTR to promote intestinal secretion and affect bone growth. It phosphorylates CFTR, coordinating cGMP-dependent translocation in the jejunum. PRKG2 Protein, Human (sf9, GST) is the recombinant human-derived PRKG2 protein, expressed by sf9 insect cells , with N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
PRKG2 protein is a key regulator that activates CFTR to promote intestinal secretion and affect bone growth. It phosphorylates CFTR, coordinating cGMP-dependent translocation in the jejunum. PRKG2 Protein, Human (sf9, GST) is the recombinant human-derived PRKG2 protein, expressed by sf9 insect cells , with N-GST labeled tag.
Background
PRKG2 protein serves as a pivotal regulator in both intestinal secretion and bone growth. Its primary role involves phosphorylating and activating CFTR on the plasma membrane, crucially influencing intestinal secretion by orchestrating the cGMP-dependent translocation of CFTR in the jejunum. Additionally, PRKG2 operates downstream of NMDAR to activate the plasma membrane accumulation of GRIA1/GLUR1 in synapses, thereby enhancing synaptic plasticity. As a multifaceted mediator, PRKG2 also acts as a regulator of gene expression and an activator of extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2 in mechanically stimulated osteoblasts. Particularly under fluid shear stress, PRKG2 mediates ERK activation, leading to the subsequent induction of FOS, FOSL1/FRA1, FOSL2/FRA2, and FOSB, crucial players in the osteoblast anabolic response to mechanical stimulation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q13237 (M1-F762)
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Gene ID5593
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Molecular Construction
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N-term
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GST
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PRKG2 (M1-F762)
Accession # Q13237 -
C-term
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Protein Length
Full Length
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Synonyms
PRKG2; CGMP-Dependent Protein Kinase 2; Protein Kinase CGMP-Dependent 2; CGK2; PRKGR2; Testicular Tissue Protein Li 99; CGKII; CGMP-Dependent Protein Kinase; PKG2; CGK 2; Protein Kinase, CGMP-Dependent, Type II; AMD4; CGMP-Dependent Protein Kinase II; SMD
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AA Sequence
MGNGSVKPKHSKHPDGHSGNLTTDALRNKVTELERELRRKDAEIQEREYHLKELREQLSKQTVAIAELTEELQNKCIQLNKLQDVVHMQGGSPLQASPDKVPLEVHRKTSGLVSLHSRRGAKAGVSAEPTTRTYDLNKPPEFSFEKARVRKDSSEKKLITDALNKNQFLKRLDPQQIKDMVECMYGRNYQQGSYIIKQGEPGNHIFVLAEGRLEVFQGEKLLSSIPMWTTFGELAILYNCTRTASVKAITNVKTWALDREVFQNIMRRTAQARDEQYRNFLRSVSLLKNLPEDKLTKIIDCLEVEYYDKGDYIIREGEEGSTFFILAKGKVKVTQSTEGHDQPQLIKTLQKGEYFGEKALISDDVRSANIIAEENDVACLVIDRETFNQTVGTFEELQKYLEGYVANLNRDDEKRHAKRSMSNWKLSKALSLEMIQLKEKVARFSSSSPFQNLEIIATLGVGGFGRVELVKVKNENVAFAMKCIRKKHIVDTKQQEHVYSEKRILEELCSPFIVKLYRTFKDNKYVYMLLEACLGGELWSILRDRGSFDEPTSKFCVACVTEAFDYLHRLGIIYRDLKPENLILDAEGYLKLVDFGFAKKIGSGQKTWTFCGTPEYVAPEVILNKGHDFSVDFWSLGILVYELLTGNPPFSGVDQMMTYNLILKGIEKMDFPRKITRRPEDLIRRLCRQNPTERLGNLKNGINDIKKHRWLNGFNWEGLKARSLPSPLQRELKGPIDHSYFDKYPPEKGMPPDELSGWDKDF
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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