PTPN12 Protein, Human (sf9)
Based on 1 Customer Validation
The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human (sf9) is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells, with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The PTPN12 protein is a multifunctional enzyme that can dephosphorylate a variety of proteins and regulate cell signaling cascades. According to literature, PTPN12 selectively targets tyrosine kinases such as ERBB2 and PTK2B/PYK2 to control related pathways. PTPN12 Protein, Human (sf9) is the recombinant human-derived PTPN12 protein, expressed by Sf9 insect cells, with tag free.
PTPN12 Protein, the subject, functions as a versatile enzyme capable of dephosphorylating a range of proteins, thereby playing a crucial role in the regulation of various cellular signaling cascades, as documented in the literature. Among its substrates, PTPN12 selectively dephosphorylates cellular tyrosine kinases, including ERBB2 and PTK2B/PYK2, exerting regulatory control over signaling pathways involving these kinases. The specificity of PTPN12 is highlighted by its ability to dephosphorylate ERBB2 at specific tyrosine residues, such as 'Tyr-1112,' 'Tyr-1196,' and/or 'Tyr-1248.' The intricate dephosphorylation activity of PTPN12 underscores its significance in modulating key signaling events and fine-tuning cellular responses mediated by tyrosine kinase pathways.
Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site.The specific activity is >20 µmol/min/mg.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q05209 (N-G&P, M1-Q355)
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Molecular Construction
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N-term
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Gly-Pro
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PTPN12 (M1-Q355)
Accession # Q05209 -
C-term
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Protein Length
Full Length
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Synonyms
PTPN12; Tyrosine-Protein Phosphatase Non-Receptor Type 12; Protein Tyrosine Phosphatase Non-Receptor Type 12; Protein-Tyrosine Phosphatase G1; PTP-PEST; Protein-Tyrosine-Phosphatase; PTPG1
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Predicted Molecular Mass
41.8 kDa
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Molecular Weight
Approximately 41 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (233 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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