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PTPRD Protein, Human (HEK293, His)

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Based on 1 Customer Validation

PTPRD protein is a receptor-type protein tyrosine phosphatase involved in various cellular processes related to cytoskeletal proteins. It regulates cell adhesion, motility, migration, cell cycle, neuronal growth, ion channel regulation, immune response, cancer cell invasion and apoptosis. PTPRD Protein, Human (HEK293, His) is the recombinant human-derived PTPRD protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for PTPRD Protein, Human (HEK293, His)
  • PTPRD Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

PTPRD protein is a receptor-type protein tyrosine phosphatase involved in various cellular processes related to cytoskeletal proteins. It regulates cell adhesion, motility, migration, cell cycle, neuronal growth, ion channel regulation, immune response, cancer cell invasion and apoptosis. PTPRD Protein, Human (HEK293, His) is the recombinant human-derived PTPRD protein, expressed by HEK293 , with C-His labeled tag.

Background

PKCE, a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase, plays vital roles in regulating various cellular processes associated with cytoskeletal proteins. It is involved in cell adhesion, motility, migration, cell cycle, neuron growth, ion channel regulation, immune response, cancer cell invasion, and apoptosis. PKCE mediates integrin-dependent signaling, influencing cell adhesion to the extracellular matrix, and phosphorylates proteins such as MARCKS, PTK2/FAK, vimentin (VIM), keratin-8 (KRT8), and IQGAP1 to modulate diverse cellular functions. It contributes to cell migration in response to growth factors, participates in cytokinesis, regulates myofilament function in cardiac myocytes, and is implicated in the pathogenesis of dilated cardiomyopathy. Additionally, PKCE influences neurite outgrowth, synaptic potentiation, and the immune response by phosphorylating various substrates, including GABRG2, TRPV1, CACNA1B, STAT3, TICAM2/TRAM, and NLRP5/MATER. It emerges as a multifaceted kinase with wide-ranging impacts on cellular physiology and pathology.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When recombinant human PTPRD is Immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated human IL1RAPL1. The ED50 for this effect is 169.5 ng/mL.

MCE Validation Data

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When recombinant human PTPRD is Immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated human IL1RAPL1. The ED50 for this effect is 169.5 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P23468-1 (E21-E1265)

  • Gene ID
    5789  [NCBI]
  • Molecular Construction
    • N-term
    • PTPRD (E21-E1265)
      Accession # P23468-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PTPRD; R-PTP-Delta; Protein Tyrosine Phosphatase Receptor Type D; Protein Tyrosine Phosphatase, Receptor Type, Delta Polypeptide; Receptor-Type Tyrosine-Protein Phosphatase Delta; Protein-Tyrosine Phosphatase Delta; PTPD; HPTPDELTA; HPTP; RPTPDELTA; Rcept

  • AA Sequence

    ETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFEVIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSDYEFRVVAVNNIGRGPPSEPVLTQTSEQAPSSAPRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIANYELVYKDGEHGEEQRITIEPGTSYRLQGLKPNSLYYFRLAARSPQGLGASTAEISARTMQSKPSAPPQDISCTSPSSTSILVSWQPPPVEKQNGIITEYSIKYTAVDGEDDKPHEILGIPSDTTKYLLEQLEKWTEYRITVTAHTDVGPGPESLSVLIRTNEDVPSGPPRKVEVEAVNSTSVKVSWRSPVPNKQHGQIRGYQVHYVRMENGEPKGQPMLKDVMLADAQWEFDDTTEHDMIISGLQPETSYSLTVTAYTTKGDGARSKPKLVSTTGAVPGKPRLVINHTQMNTALIQWHPPVDTFGPLQGYRLKFGRKDMEPLTTLEFSEKEDHFTATDIHKGASYVFRLSARNKVGFGEEMVKEISIPEEVPTGFPQNLHSEGTTSTSVQLSWQPPVLAERNGIITKYTLLYRDINIPLLPMEQLIVPADTTMTLTGLKPDTTYDVKVRAHTSKGPGPYSPSVQFRTLPVDQVFAKNFHVKAVMKTSVLLSWEIPENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAFIGKTNLDGMITVQLPEVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMEHAESKMYATSPYSDPVVSMDLDPQPITDEEE

  • Predicted Molecular Mass

    139.5 kDa

  • Molecular Weight

    Approximately 116-130 kDa & 12-14 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When recombinant human PTPRD is Immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated human IL1RAPL1. The ED50 for this effect is 169.5 ng/mL.

  • PTPRD Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for PTPRD Protein, Human (HEK293, His)
  • PTPRD Protein, Human (HEK293, His) Product Image