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  4. RAB11B Protein, Human (HEK293, His)

RAB11B Protein, Human (HEK293, His)

Cat. No.: HY-P74610
COA Handling Instructions

RAB11B protein is an important small GTPase in the Rab family, coordinating intracellular membrane transport by regulating vesicle processes. It cycles between GDP and GTP forms, recruiting effectors to control activities such as vesicle formation and movement. RAB11B Protein, Human (HEK293, His) is the recombinant human-derived RAB11B protein, expressed by HEK293 , with N-His labeled tag. The total length of RAB11B Protein, Human (HEK293, His) is 214 a.a., with molecular weight of ~25-30 kDa.

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  • Biological Activity

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Description

RAB11B protein is an important small GTPase in the Rab family, coordinating intracellular membrane transport by regulating vesicle processes. It cycles between GDP and GTP forms, recruiting effectors to control activities such as vesicle formation and movement. RAB11B Protein, Human (HEK293, His) is the recombinant human-derived RAB11B protein, expressed by HEK293 , with N-His labeled tag. The total length of RAB11B Protein, Human (HEK293, His) is 214 a.a., with molecular weight of ~25-30 kDa.

Background

The small GTPase RAB11B, a member of the Rab family, plays a pivotal role in intracellular membrane trafficking, orchestrating processes ranging from the formation of transport vesicles to their fusion with membranes. This GTPase undergoes a cycle between an inactive GDP-bound form and an active GTP-bound form, recruiting distinct downstream effectors that directly govern vesicle activities, such as formation, movement, tethering, and fusion. Specifically, RAB11B is implicated in endocytic recycling, where it regulates the apical recycling of transmembrane proteins, including cystic fibrosis transmembrane conductance regulator (CFTR), epithelial sodium channel (ENaC), potassium voltage-gated channels, and voltage-dependent L-type calcium channels. Furthermore, it participates in the control of constitutive and regulated secretion, such as insulin granule exocytosis. RAB11B is essential for melanosome transport and release from melanocytes and is involved in regulating V-ATPase intracellular transport in response to extracellular acidosis. Additionally, it promotes Rabin8/RAB3IP preciliary vesicular trafficking to the mother centriole, forming a ciliary targeting complex that includes Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3, and ARF4, thereby regulating the initiation of ciliogenesis. Conversely, under LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 inhibits Rab11-RAB3IP-RAB11FIP3 complex formation and suppresses cilia growth.

Biological Activity

Measured by its ability to catalyze the substrate GTP. The specific activity is 0.27 nmol/min/mg.

Species

Human

Source

HEK293

Tag

N-6*His

Accession

Q15907-1 (G2-C215)

Gene ID
Molecular Construction
N-term
His
RAB11B (G2-C215)
Accession # Q15907
C-term
Synonyms
Ras-related protein Rab-11B; GTP-binding protein YPT3; RAB11B; YPT3
AA Sequence

GTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNNLSFIETSALDSTNVEEAFKNILTEIYRIVSQKQIADRAAHDESPGNNVVDISVPPTTDGQKPNKLQCC

Molecular Weight

Approximately 25-30 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

RAB11B Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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RAB11B Protein, Human (HEK293, His)
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HY-P74610
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