RGMA Protein, Human (Biotinylated, HEK293, His-Avi)
The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-Avi, C-His labeled tag.
Background
RGMA Protein, a member of the repulsive guidance molecule (RGM) family, plays diverse roles in both the developing and adult nervous system. It governs cephalic neural tube closure, restrains neurite outgrowth, inhibits cortical neuron branching, and modulates the formation of mature synapses. Upon binding to its receptor NEO1/neogenin, RGMA triggers the activation of the RHOA-ROCK1/Rho-kinase signaling pathway through the UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, resulting in the collapse of the neuronal growth cone and inhibition of neurite outgrowth. Additionally, RGMA's interaction with NEO1/neogenin influences the HRAS-PTK2/FAK1-AKT1 pathway, leading to HRAS inactivation. Acting as a bone morphogenetic protein (BMP) coreceptor, RGMA may signal through SMAD1, SMAD5, and SMAD8. Its intricate network of interactions, including those with NEO1, BMP2, and BMP4, underscores RGMA's significance in orchestrating key molecular events essential for nervous system development and function.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-Avi;C-8*His
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Accession
Q96B86-1 (C48-G422)
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Molecular Construction
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N-term
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RGMA (C48-G422)
Accession # Q96B86-1 -
8*His-Avi
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C-term
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Protein Length
Partial
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Conjugation
Biotin
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Synonyms
RGMA; Repulsive Guidance Molecule Family Member A; Repulsive Guidance Molecule BMP Co-Receptor A; RGM Domain Family, Member A; RGM; RGM Domain Family Member A; Repulsive Guidance Molecule A; RGMA Protein
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AA Sequence
CKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG
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Predicted Molecular Mass
44.5 kDa
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Molecular Weight
Approximately 27-35 kDa & 40-50 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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