1. Recombinant Proteins
  2. Receptor Proteins
  3. RGMA Protein, Human (HEK293, His)

RGMA Protein, Human (HEK293, His)

Cat. No.: HY-P76572
COA Handling Instructions

The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (HEK293, His) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-His labeled tag. The total length of RGMA Protein, Human (HEK293, His) is 422 a.a., with molecular weight of ~36 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
50 μg $340 In-stock
100 μg $580 Get quote
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (HEK293, His) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-His labeled tag. The total length of RGMA Protein, Human (HEK293, His) is 422 a.a., with molecular weight of ~36 kDa.

Background

RGMA Protein, a member of the repulsive guidance molecule (RGM) family, plays diverse roles in both the developing and adult nervous system. It governs cephalic neural tube closure, restrains neurite outgrowth, inhibits cortical neuron branching, and modulates the formation of mature synapses. Upon binding to its receptor NEO1/neogenin, RGMA triggers the activation of the RHOA-ROCK1/Rho-kinase signaling pathway through the UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, resulting in the collapse of the neuronal growth cone and inhibition of neurite outgrowth. Additionally, RGMA's interaction with NEO1/neogenin influences the HRAS-PTK2/FAK1-AKT1 pathway, leading to HRAS inactivation. Acting as a bone morphogenetic protein (BMP) coreceptor, RGMA may signal through SMAD1, SMAD5, and SMAD8. Its intricate network of interactions, including those with NEO1, BMP2, and BMP4, underscores RGMA's significance in orchestrating key molecular events essential for nervous system development and function.

Species

Human

Source

HEK293

Tag

C-His

Accession

Q96B86 (M1-G422)

Gene ID
Molecular Construction
N-term
RGMA (M1-G422)
Accession # Q96B86
His
C-term
Synonyms
Repulsive guidance molecule A; RGM domain family member A; RGMA; RGM
Molecular Weight

Approximately 36 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

RGMA Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
RGMA Protein, Human (HEK293, His)
Cat. No.:
HY-P76572
Quantity:
MCE Japan Authorized Agent: