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RGMA Protein, Human (HEK293, His)

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The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (HEK293, His) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (HEK293, His) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-His labeled tag.

Background

RGMA Protein, a member of the repulsive guidance molecule (RGM) family, plays diverse roles in both the developing and adult nervous system. It governs cephalic neural tube closure, restrains neurite outgrowth, inhibits cortical neuron branching, and modulates the formation of mature synapses. Upon binding to its receptor NEO1/neogenin, RGMA triggers the activation of the RHOA-ROCK1/Rho-kinase signaling pathway through the UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, resulting in the collapse of the neuronal growth cone and inhibition of neurite outgrowth. Additionally, RGMA's interaction with NEO1/neogenin influences the HRAS-PTK2/FAK1-AKT1 pathway, leading to HRAS inactivation. Acting as a bone morphogenetic protein (BMP) coreceptor, RGMA may signal through SMAD1, SMAD5, and SMAD8. Its intricate network of interactions, including those with NEO1, BMP2, and BMP4, underscores RGMA's significance in orchestrating key molecular events essential for nervous system development and function.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q96B86-1 (C48-G422)

  • Gene ID
    56963  [NCBI]
  • Molecular Construction
    • N-term
    • RGMA (C48-G422)
      Accession # Q96B86-1
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    RGMA; Repulsive Guidance Molecule Family Member A; Repulsive Guidance Molecule BMP Co-Receptor A; RGM Domain Family, Member A; RGM; RGM Domain Family Member A; Repulsive Guidance Molecule A; RGMA Protein

  • AA Sequence

    CKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYERTRDLPG

  • Predicted Molecular Mass

    43 kDa

  • Molecular Weight

    Approximately 36 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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