SARS-CoV-2 3CLpro/3C-like protease Protein

SARS-CoV-2 3CLpro/3C-like protease Protein is the recombinant Virus-derived SARS-CoV-2 3CLpro/3C-like protease protein, expressed by E. coli , with tag free. The total length of SARS-CoV-2 3CLpro/3C-like protease Protein is 306 a.a., with molecular weight of ~33.8 kDa.

Measured by its ability to cleave a fluorogenic peptide substrate Dabcyl-KTSAVLQSGFRKME-Edans and the specific activity is ≥1068.3 nmol/min/mg.

Virus

E. coli

Tag Free

YP_009725301.1 (S1-Q306)

43740578  [NCBI]

Approximately 33.8 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 25 mM HEPES, 2.5 mM DTT, 10% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.