SARS-CoV-2 3CLpro/3C-like protease Protein
Based on 1 Customer Validation
SARS-CoV-2 3CLpro/3C-like protease is a viral cysteine protease that plays a crucial role in the co-translational proteolytic processing of coronavirus polyproteins. Since no homologous proteins with similar cleavage sites exist in the human body, SARS-CoV-2 3CLpro/3C-like protease is a high-quality target for the development of drugs related to COVID-19. SARS-CoV-2 3CLpro/3C-like protease protein is a recombinant, untagged SARS-CoV-2 3CLpro/3C-like protease protein expressed by E.coli.
- Species: Virus
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
SARS-CoV-2 3CLpro/3C-like protease is a viral cysteine protease that plays a crucial role in the co-translational proteolytic processing of coronavirus polyproteins. Since no homologous proteins with similar cleavage sites exist in the human body, SARS-CoV-2 3CLpro/3C-like protease is a high-quality target for the development of drugs related to COVID-19. SARS-CoV-2 3CLpro/3C-like protease protein is a recombinant, untagged SARS-CoV-2 3CLpro/3C-like protease protein expressed by E.coli[1][2][3].
Background
SARS-CoV-2 3CLpro/3C-like protease protein is a cysteine protease composed of three domains. Within the cleft between domain I and domain II, it contains the Cys145-His41 catalytic dimer, which cleaves pp1a and pp1ab to generate 12 functional proteins responsible for viral genome replication and transcription. SARS-CoV-2 3CLpro/3C-like protease protein lacks homologous proteins with similar cleavage sites in the human body, effectively reducing off-target risks. Its catalytic pocket is an ideal target for developing anti-coronavirus inhibitors. SARS-CoV-2 3CLpro/3C-like protease protein recognizes its peptide substrates according to the Schechter-Berger nomenclature, with the substrate cleavage site located between P1 and P1'. SARS-CoV-2 3CLpro/3C-like protease protein relies on catalytic dimerization to complete substrate hydrolysis through a multi-step chemical reaction, and can regain its activity after catalysis. Inhibitors of SARS-CoV-2 3CLpro/3C-like protease protein are mainly divided into two categories: covalent inhibitors and non-covalent inhibitors[1][2][3].
In Vitro
SARS-CoV-2 3CLpro/3C-like protease protein and mutant SARS coronavirus 3CLpro (Q189) are both inhibited by Quercetin (HY-18085), with IC50 values of 42.79 μM and 27.89 μM respectively[2].
SARS-CoV-2 3CLpro/3C-like protease protein is strongly inhibited by 73 μM Quercetin. This concentration of Quercetin also inhibits recombinant SARS coronavirus 3CLpro expressed by Pichia pastoris, with the inhibition rate of catalytic activities exceeding 80% for both proteins[2].
SARS-CoV-2 3CLpro/3C-like protease protein can be bound and inhibited by various quercetin glycosides including Rutin (HY-N0148). Quercetin and its metabolites target the dimerization site and regulatory site of this protease to block its function[2].
SARS-CoV-2 3CLpro/3C-like protease protein is reversibly and competitively inhibited by 4.0 nM EDP-235 (HY-156651), with an apparent inhibition constant (Kiapp) of 3.0 nM. EDP-235 can also inhibit 3CLpro derived from SARS coronavirus, human coronavirus and zoonotic coronavirus, which are homologous to SARS-CoV-2 3CLpro/3C-like protease protein[3].
Verified Bioactivity
Measured by its ability to cleave a fluorogenic peptide substrate Dabcyl-KTSAVLQSGFRKME-Edans and the specific activity is ≥1068.3 nmol/min/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Virus
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Source E. coli
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Tag Tag Free
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Accession
YP_009725301.1 (S1-Q306)
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Gene ID43740578 [NCBI]
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Molecular Construction
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N-term
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SARS-CoV-2 3CLpro (S1-Q306)
Accession # YP_009725301.1 -
C-term
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Protein Length
Full Length
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Synonyms
3C-like protease; 3CL Protease; 3CL-Mpro; 3CL Pro; COVID-19; M Proteinase; Mpro
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AA Sequence
SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ
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Predicted Molecular Mass
33.8 kDa
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Molecular Weight
Approximately 34 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 25 mM HEPES, 2.5 mM DTT, 10% glycerol, pH 7.5.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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