Serpin E1 Protein, Rat (HEK293, His)
Based on 1 Customer Validation
Serpin E1 Protein, Rat (HEK293, His) is one important member of the serine proteinase inhibitor E superfamily that plays a crucial role in the fibrinolytic system. The Serpin E1 protein is a single-chain glycoprotein composed of 379 amino acids. The active site of the protein is Arg346-Met347. SERPINE1 encodes plasminogen activator inhibitor type 1 (PAI-1), which serves as the primary inhibitor of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). The Serpin E1 protein can quickly inhibit the formation of plasmin. Serpin E1 can be transcribed through several signaling cascades, including pro-fibrogenic and pro-inflammatory pathways. Serpin E1 is a serine proteinase inhibitor, serves as an important regulator of extracellular matrix remodeling. Serpin E1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin E1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag.
- Species: Rat
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Serpin E1 Protein, Rat (HEK293, His) is one important member of the serine proteinase inhibitor E superfamily that plays a crucial role in the fibrinolytic system. The Serpin E1 protein is a single-chain glycoprotein composed of 379 amino acids. The active site of the protein is Arg346-Met347. SERPINE1 encodes plasminogen activator inhibitor type 1 (PAI-1), which serves as the primary inhibitor of urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA). The Serpin E1 protein can quickly inhibit the formation of plasmin. Serpin E1 can be transcribed through several signaling cascades, including pro-fibrogenic and pro-inflammatory pathways. Serpin E1 is a serine proteinase inhibitor, serves as an important regulator of extracellular matrix remodeling[1][2][3]. Serpin E1 Protein, Rat (HEK293, His) is the recombinant rat-derived Serpin E1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag.
The SERPINE1 protein is a single-chain glycoprotein composed of 379 amino acids that are mainly synthesized and secreted by platelets, megakaryocytes, hepatocytes, adipocytes, smooth muscle cells, and vascular endothelial cells. The active site of the protein is Arg346-Met347. The half-life of activated SERPINE1 at 37°C is 1–2 h, transforming spontaneously into the latent form. in the fibrinolytic system, SERPINE1 plays a physiological role as an inhibitor of fibrinogen activators. SerpinE1 drives a cell-autonomous pathogenic signaling in Hutchinson-Gilford progeria syndrome[1].
SERPINE1 was identified as a regulator of glioblastoma cell dispersal and downregulation of SERPINE1 limited the proliferation and invasion of glioma cells[3].
Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 80 nM.
Technical Parameters
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Species Rat
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Source HEK293
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Tag C-His
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Accession
NP_036752.1 (S24-P402)
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Molecular Construction
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N-term
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Serpin E1 (S24-P402)
Accession # NP_036752.1 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
SERPINE1; Endothelial Plasminogen Activator Inhibitor; Prev. PLANH1; Serpin E1; Prev. PAI1; PAI-1; PAI; Serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1; Serine (Or Cysteine) Proteinase Inhibitor, Clade E (Nexi
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AA Sequence
SPLPESHTAQQATNFGVKVFQHVVQASKDRNVVFSPYGVSSVLAMLQLTTAGKTRQQIQDAMGFNISERGTAPALRKLSKELMGSWNKNEISTADAIFVQRDLELVQGFMPHFFKLFRTTVKQVDFSEVERARFIINDWVERHTKGMISDLLAKGAVNELTRLVLVNALYFNGQWKTPFLEASTHQRLFHKSDGSTISVPMMAQNNKFNYTEFTTPDGHEYDILELPYHGETLSMFIAAPFEKDVPLSAITNILDAELIRQWKSNMTRLPRLLILPKFSLETEVDLRGPLEKLGMTDIFSSTQADFTSLSDQEQLSVAQALQKVKIEVNESGTVASSSTAILVSARMAPTEMVLDRSFLFVVRHNPTETILFMGQLMEP
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Molecular Weight
Approximately 48-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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