SLC29A2 Protein, Human (HEK293, His, MBP, FLAG)

The bidirectional uniporter SLC29A2 protein plays a crucial role in facilitating the transport of nucleosides and nucleobases, contributing to the maintenance of their cellular homeostasis. Operating as a Na(+)-independent, passive transporter, SLC29A2 is involved in the transport of various nucleosides, including inosine, adenosine, uridine, thymidine, cytidine, and guanosine, as well as purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). The protein participates in nucleoside transport at the basolateral membrane of kidney cells, facilitating the liver absorption of nucleoside metabolites. Additionally, SLC29A2 mediates apical nucleoside uptake into Sertoli cells, regulating nucleoside transport in the testis across the blood-testis barrier. In skeletal muscle microvascular endothelial cells, it orchestrates both the influx and efflux of hypoxanthine, thereby controlling the intracellular hypoxanthine levels available for xanthine oxidase-mediated reactive oxygen species (ROS) production. Notably, SLC29A2 does not function as a nucleoside transporter protein for adenosine or thymidine transport and does not express on the cell membrane.