SMAD2 Protein, Human (His-SUMO)

SMAD2 protein, an R-SMAD, acts as a transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds TRE elements, regulating TGF-beta target genes. Forming a complex with SMAD4, it promotes transcription, positively regulating PDPK1 and showing potential tumor suppressor functions in colorectal carcinoma. SMAD2 interacts with various proteins, participating in signal transduction and transcriptional regulation. SMAD2 Protein, Human (His-SUMO) is the recombinant human-derived SMAD2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of SMAD2 Protein, Human (His-SUMO) is 466 a.a., with molecular weight of ~68.2 kDa.

The SMAD2 protein functions as a receptor-regulated SMAD (R-SMAD), serving as an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. It binds the TRE element in the promoter region of numerous genes regulated by TGF-beta, and upon forming the SMAD2/SMAD4 complex, it activates transcription. SMAD2 plays a role in promoting TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells. Additionally, it positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ, acting as a negative regulator. There is evidence suggesting its potential function as a tumor suppressor in colorectal carcinoma. SMAD2 exists as a monomer in the absence of TGF-beta and forms a heterodimer with co-SMAD, SMAD4, in the nucleus to constitute the transactivation complex SMAD2/SMAD4. It interacts with various proteins, including ZFYVE9, TAZ/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, RANBP3, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-beta, TGFBR1, TGIF, TRIM33, ZNF580, NEDD4L, HGS, AIP1, WWP1, PML, ZNF8, RNF111, and YAP1, participating in diverse cellular processes, including signal transduction, transcriptional regulation, and protein-protein interactions.

Human

E. coli

N-SUMO;N-6*His

Q15796 (S2-S467)

4087  [NCBI]

SSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Approximately 68.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.