SMARCA4 Protein, Human (P.pastoris, His)

The SMARCA4 protein is a multifaceted regulator of chromatin remodeling that dynamically alters DNA nucleosome topology through ATP-dependent enzymatic activity. As part of the SWI/SNF complex and the CREST-BRG1 complex, it regulates transcriptional processes, coordinating the release and activating recruitment of calcium-dependent repressor complexes. SMARCA4 Protein, Human (P.pastoris, His) is the recombinant human-derived SMARCA4 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of SMARCA4 Protein, Human (P.pastoris, His) is 547 a.a., with molecular weight of ~64.8 kDa.

The SMARCA4 Protein is a multifaceted regulator involved in both transcriptional activation and repression through chromatin remodeling, contributing to alterations in DNA-nucleosome topology. As a component of SWI/SNF chromatin remodeling complexes, it exerts ATP-dependent enzymatic activities that dynamically modify chromatin structure by influencing DNA-histone contacts within nucleosomes. In the context of the CREST-BRG1 complex, SMARCA4 participates in orchestrating calcium-dependent release of repressor complexes and recruitment of activator complexes, modulating transcriptional processes. Particularly in neural development, SMARCA4 is crucial for the switch from proliferating neural stem/progenitor cells to postmitotic neurons, acting as a corepressor of ZEB1 and facilitating E-cadherin transcription. Additionally, it engages in diverse interactions with various proteins, contributing to its involvement in essential cellular functions, including growth regulation, chromatin remodeling, and transcriptional control. The intricate role of SMARCA4 in these processes highlights its significance in cellular differentiation, chromatin dynamics, and gene expression regulation.

Human

P. pastoris

N-6*His

P51532 (E700-F1246)

6597  [NCBI]

EVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAF

Approximately 64.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.