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SOD1 Protein, Human (N-His)

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Based on 1 Customer Validation

SOD1 Protein, an enzyme crucial for cellular defense, operates as a superoxide dismutase, neutralizing radicals to safeguard cells from oxidative stress.Its enzymatic activity underscores the importance of SOD1 in maintaining cellular homeostasis and protecting biological systems from the harmful effects of reactive oxygen species accumulation.SOD1 Protein, Human (N-His) is the recombinant human-derived SOD1 protein, expressed by E.coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SOD1 Protein, Human (N-His)
  • SOD1 Protein, Human (N-His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

SOD1 Protein, an enzyme crucial for cellular defense, operates as a superoxide dismutase, neutralizing radicals to safeguard cells from oxidative stress.Its enzymatic activity underscores the importance of SOD1 in maintaining cellular homeostasis and protecting biological systems from the harmful effects of reactive oxygen species accumulation.SOD1 Protein, Human (N-His) is the recombinant human-derived SOD1 protein, expressed by E.coli , with N-6*His labeled tag.

Background

The subject, SOD1 Protein, functions as an enzyme crucial for cellular defense, as it plays a vital role in neutralizing radicals that are typically generated within cells and can be toxic to biological systems. Operating as a superoxide dismutase, SOD1 effectively destroys these radicals, thereby safeguarding cells from oxidative stress and potential damage caused by the accumulation of reactive oxygen species. The enzymatic activity of SOD1 underscores its importance in maintaining cellular homeostasis and protecting biological systems from the harmful effects of oxidative stress.

Verified Bioactivity

Specific actifity is 148.432 units/mg. When the inhibition percentage in the xanthine oxidase coupled reaction system mentioned above is 50%, the SOD enzyme activity in the reaction system is defined as one enzyme activity unit.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag N-6*His
  • Accession

    P00441 (A2-Q154)

  • Gene ID
    6647
  • Molecular Construction
    • N-term
    • 6*His
    • SOD1 (M1-Q154)
      Accession # P00441
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    SOD1; Superoxide Dismutase, Cystolic; Prev. ALS1; Cu/Zn Superoxide Dismutase; Prev. ALS; Alternative Protein SOD1; Superoxide Dismutase [Cu-Zn]; Superoxide Dismutase; IPOA; Indophenoloxidase A; Superoxide Dismutase 1, Soluble; SOD, Soluble; Hydrogen Sulfi

  • AA Sequence

    ATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

  • Predicted Molecular Mass

    15.8 kDa

  • Molecular Weight

    Approximately 20-22 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • SOD1 Protein, Human (N-His) Product Image
    1/16
  • Experimental Validation Results for SOD1 Protein, Human (N-His)
  • SOD1 Protein, Human (N-His) Product Image