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SPARCL1 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for SPARCL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for SPARCL1 Protein, Mouse (HEK293, His)
  • SPARCL1 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. SPARCL1 mainly plays a role in glutamatergic synaptic membrane adhesion. SPARCL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag.

Background

SPARCL1 protein is a multifunctional molecule with calcium ion, collagen and extracellular matrix binding activities. It is mainly localized at glutamatergic synapses and participates in the synaptic membrane adhesion process. SPARCL1 is widely expressed in multiple systems, including cardiovascular, central nervous, male reproductive, respiratory and sensory organ systems, and is particularly highly expressed in adult bladder and cortex, suggesting that it plays a key role in these tissues. The protein is highly conserved in evolution, and the existence of its human homolog SPARC like 1 further confirms its functional importance.

Verified Bioactivity

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×103 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    EDL20231.1 (I17-F650)

  • Gene ID
    13602  [NCBI]
  • Molecular Construction
    • N-term
    • SPARCL1 (I17-F650)
      Accession # EDL20231.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    SPARCL1; Hevin; SPARC Like 1; PIG33; SPARC-Like Protein 1; Proliferation-Inducing Protein 33; MAST9; SPARC-Like 1 (Mast9, Hevin); High Endothelial Venule Protein; SPARC-Like 1 (Hevin); MAST 9; SC1

  • AA Sequence

    IPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPNDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEEEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

  • Predicted Molecular Mass

    72.2 kDa

  • Molecular Weight

    Approximately 118 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×103 units/mg.

  • SPARCL1 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for SPARCL1 Protein, Mouse (HEK293, His)
  • Experimental Validation Results for SPARCL1 Protein, Mouse (HEK293, His)
  • SPARCL1 Protein, Mouse (HEK293, His) Product Image