SPARCL1 Protein, Mouse (650a.a, HEK293, His)

The SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. It plays a role in synaptic membrane adhesion, primarily at glutamatergic synapses. SPARCL1 Protein, Mouse (650a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of SPARCL1 Protein, Mouse (650a.a, HEK293, His) is 634 a.a., with molecular weight of ~95-100 kDa.

SPARCL1 protein is predicted to possess crucial functions, including calcium ion binding activity, collagen binding activity, and extracellular matrix binding activity. Playing a role in synaptic membrane adhesion, it is prominently located in glutamatergic synapses. The expression profile of SPARCL1 spans across diverse structures, encompassing the cardiovascular system, central nervous system, male reproductive gland or organ, respiratory system, and sensory organ. This protein's biased expression, particularly elevated in the adult bladder and cortex, highlights its significance in these tissues, suggesting its involvement in various physiological processes. The conservation of SPARCL1 across species, as seen in its orthologous counterpart SPARC like 1 in humans, underscores its evolutionary importance and potential functional conservation.

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×10³ units/mg.

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  Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×10³ units/mg.

Mouse

HEK293

C-His;C-10*His

EDL20231.1 (I17-F650)

13602  [NCBI]

IPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPNDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEEEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Approximately 95-100 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.