SULT2B1 Protein, Human (His)
Based on 1 publication(s) in Google Scholar
The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA. It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation. SULT2B1 Protein, Human (His) is the recombinant human-derived SULT2B1 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The SULT2B1 protein uses PAPS to catalyze sulfate conjugation, mainly sulfating cholesterol, and shows significant activity on steroids such as pregnenolone and DHEA. It plays a crucial role in epidermal cholesterol metabolism, regulating epidermal proliferation and differentiation. SULT2B1 Protein, Human (His) is the recombinant human-derived SULT2B1 protein, expressed by E. coli , with N-His labeled tag.
Background
SULT2B1 protein, a sulfotransferase utilizing 3'-phospho-5'-adenylyl sulfate (PAPS) as its sulfonate donor, holds a pivotal role in catalyzing sulfate conjugation, with a primary responsibility for sulfating cholesterol. Additionally, SULT2B1 demonstrates significant activity in sulfating steroids, particularly the 3beta-hydroxyl groups of molecules such as pregnenolone and dehydroepiandrosterone (DHEA). While it exhibits a preferential sulfonation of cholesterol, it also displays substantial activity with pregnenolone and DHEA. Beyond its enzymatic functions, SULT2B1 plays a crucial role in epidermal cholesterol metabolism, contributing to the regulation of epidermal proliferation and differentiation. Notably, it sulfonates pregnenolone but not cholesterol, underscoring its substrate selectivity and emphasizing its multifaceted involvement in the intricate processes governing cholesterol homeostasis and steroid metabolism.
Verified Bioactivity
Measured by its ability to transfer sulfate from PAPS to pregnenolone. The specific activity is > 10 pmol/min/μg that incubate at 37 °C for 30 minutes.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (1)
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Journal Impact Factor
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Most Recent
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J Transl Med
SULT2B1: a novel therapeutic target in colorectal cancer via modulation of AKT/PKM2-mediated glycolysis and proliferation. [Abstract]2024 Dec 2;22(1):1093. PMID: 39623433
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
O00204-1 (D2-S365)
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Molecular Construction
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N-term
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His
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SULT2B1 (D2-S365)
Accession # O00204-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
SULT2B1; Sulfotransferase 2B1; Sulfotransferase Family 2B Member 1; ST2B1; HSST2; Sulfotransferase Family Cytosolic 2B Member 1; Sulfotransferase Family, Cytosolic, 2B, Member 1; Hydroxysteroid Sulfotransferase SULT2B1a; Hydroxysteroid Sulfotransferase 2;
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AA Sequence
DGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESISLAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWERAPWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPRDVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMKGKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMKANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQMRGMPTFPWDEDPEEDGSPDPEPSPEPEPKPSLEPNTSLEREPRPNSSPSPSPGQASETPHPRPS
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Predicted Molecular Mass
41.2 kDa
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Molecular Weight
Approximately 44 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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