Tau/MAPT Protein, Macaca mulatta (HEK293, His)

Tau/MAPT Protein crucially promotes microtubule assembly and stability, essential for neuronal polarity. Structurally, its C-terminus binds axonal microtubules, and the N-terminus engages neural plasma membrane components, suggesting a role as a linker protein connecting microtubules to the plasma membrane. Tau's localization dictates axonal polarity, particularly near the centrosome. Short isoforms offer cytoskeletal plasticity, while longer isoforms stabilize microtubules. Tau interacts with proteins like MARK1, MARK2, SQSTM1, and LRP1, revealing its multifaceted role in cellular processes. Tau/MAPT Protein, Macaca mulatta (HEK293, His) is the recombinant Rhesus Macaque-derived Tau/MAPT protein, expressed by HEK293, with N-10*His labeled tag. The total length of Tau/MAPT Protein, Macaca mulatta (HEK293, His) is 458 a.a., with molecular weight of 50.6 kDa.

Tau/MAPT Protein plays a crucial role in cellular dynamics by promoting microtubule assembly and stability, contributing to the establishment and maintenance of neuronal polarity. Its structural organization reveals a functional duality, with the C-terminus binding to axonal microtubules and the N-terminus engaging neural plasma membrane components. This arrangement suggests that tau serves as a linker protein, bridging the connection between microtubules and the plasma membrane in neurons. The localization of tau within the neuronal cell dictates axonal polarity, specifically in the region defined by the centrosome. Notably, the short isoforms offer cytoskeletal plasticity, while the longer isoforms likely play a preferential role in stabilization. Tau engages in a network of interactions with various proteins, including MARK1, MARK2, MARK3, MARK4, SQSTM1, PSMC2, FKBP4, CSNK1D, SGK1, PIN1, and LRRK2, illustrating its multifaceted involvement in cellular processes. Additionally, its interaction with LRP1, leading to endocytosis, highlights the intricate regulatory mechanisms governing tau's functional versatility.

Rhesus Macaque

HEK293

N-10*His

P57786 (A2-L459)

574327  [NCBI]

AEPRQEFDVMEDHAGTYGLGDRKDQEGYTMLQDQEGDTDAGLKESPLQTPAEDGSEELGSETSDAKSTPTAEDVTAPLVDERAPGEQAAAQPHMEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSATKQVQRKPPPAEPTSERGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPAREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL

50.6 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.