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Thrombomodulin Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Thrombomodulin is an endothelial cell receptor that forms a 1:1 complex with thrombin and is essential for the activation of protein C to protein Ca.Protein Ca cleaves coagulation cascade cofactors and reduces thrombin generation.Thrombomodulin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thrombomodulin protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Thrombomodulin Protein, Mouse (HEK293, His)
  • Thrombomodulin Protein, Mouse (HEK293, His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Thrombomodulin is an endothelial cell receptor that forms a 1:1 complex with thrombin and is essential for the activation of protein C to protein Ca.Protein Ca cleaves coagulation cascade cofactors and reduces thrombin generation.Thrombomodulin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thrombomodulin protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Thrombomodulin Protein serves as a specific endothelial cell receptor, engaging in a 1:1 stoichiometric complex with thrombin. This complex plays a pivotal role in the conversion of protein C to its activated form, protein Ca. Upon activation, protein Ca functions to cleave the activated cofactors of the coagulation cascade, namely factor Va and factor VIIIa, leading to a reduction in the overall production of thrombin. Thrombomodulin's interactions with ITGAL, ITGAM, and ITGB2 further emphasize its role in mediating cellular interactions, possibly influencing immune responses. This intricate interplay underscores the significance of Thrombomodulin in modulating the coagulation pathway and maintaining hemostatic balance.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    P15306 (L17-S517)

  • Gene ID
    21824  [NCBI]
  • Molecular Construction
    • N-term
    • Thrombomodulin (L17-S517)
      Accession # P15306
    • 6*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    THBD; CD141 Antigen; Thrombomodulin; TM; THRM; THPH12; Fetomodulin; AHUS6; BDCA-3; BDCA3; CD141

  • AA Sequence

    LSALAKLQPTGSQCVEHECFALFQGPATFLDASQACQRLQGHLMTVRSSVAADVISLLLSQSSMDLGPWIGLQLPQGCDDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQTAPLCGPLCVTVSTATEAAPGEPAWEEKPCETETQGFLCEFYFTASCRPLTVNTRDPEAAHISSTYNTPFGVSGADFQTLPVGSSAAVEPLGLELVCRAPPGTSEGHWAWEATGAWNCSVENGGCEYLCNRSTNEPRCLCPRDMDLQADGRSCARPVVQSCNELCEHFCVSNAEVPGSYSCMCETGYQLAADGHRCEDVDDCKQGPNPCPQLCVNTKGGFECFCYDGYELVDGECVELLDPCFGSNCEFQCQPVSPTDYRCICAPGFAPKPDEPHKCEMFCNETSCPADCDPNSPTVCECPEGFILDEGSVCTDIDECSQGECFTSECRNFPGSYECICGPDTALAGQISKDCDPIPVREDTKEEEGSGEPPVSPTPGSPTGPPSARPVHS

  • Predicted Molecular Mass

    54.6 kDa

  • Molecular Weight

    Approximately 80-98 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 .
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Thrombomodulin Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Thrombomodulin Protein, Mouse (HEK293, His)
  • Thrombomodulin Protein, Mouse (HEK293, His) Product Image