1. Recombinant Proteins
  2. CD Antigens
  3. Macrophage CD Proteins Dendritic Cell CD Proteins Endothelial cell CD Proteins
  4. BDCA-3/CD141
  5. Thrombomodulin Protein, Mouse (HEK293, His)

Thrombomodulin Protein, Mouse (HEK293, His)

Cat. No.: HY-P72451
COA Handling Instructions

Thrombomodulin is an endothelial cell receptor that forms a 1:1 complex with thrombin and is essential for the activation of protein C to protein Ca. Protein Ca cleaves coagulation cascade cofactors and reduces thrombin generation. Thrombomodulin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thrombomodulin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Thrombomodulin Protein, Mouse (HEK293, His) is 501 a.a., with molecular weight of 85-90 kDa.

For research use only. We do not sell to patients.

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Description

Thrombomodulin is an endothelial cell receptor that forms a 1:1 complex with thrombin and is essential for the activation of protein C to protein Ca. Protein Ca cleaves coagulation cascade cofactors and reduces thrombin generation. Thrombomodulin Protein, Mouse (HEK293, His) is the recombinant mouse-derived Thrombomodulin protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Thrombomodulin Protein, Mouse (HEK293, His) is 501 a.a., with molecular weight of 85-90 kDa.

Background

Thrombomodulin Protein serves as a specific endothelial cell receptor, engaging in a 1:1 stoichiometric complex with thrombin. This complex plays a pivotal role in the conversion of protein C to its activated form, protein Ca. Upon activation, protein Ca functions to cleave the activated cofactors of the coagulation cascade, namely factor Va and factor VIIIa, leading to a reduction in the overall production of thrombin. Thrombomodulin's interactions with ITGAL, ITGAM, and ITGB2 further emphasize its role in mediating cellular interactions, possibly influencing immune responses. This intricate interplay underscores the significance of Thrombomodulin in modulating the coagulation pathway and maintaining hemostatic balance.

Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

P15306 (L17-S517)

Gene ID

21824  [NCBI]

Molecular Construction
N-term
Thrombomodulin (L17-S517)
Accession # P15306
6*His
C-term
Synonyms
Thrombomodulin; TM; Fetomodulin; CD141; BDCA-3; Thbd
AA Sequence

LSALAKLQPTGSQCVEHECFALFQGPATFLDASQACQRLQGHLMTVRSSVAADVISLLLSQSSMDLGPWIGLQLPQGCDDPVHLGPLRGFQWVTGDNHTSYSRWARPNDQTAPLCGPLCVTVSTATEAAPGEPAWEEKPCETETQGFLCEFYFTASCRPLTVNTRDPEAAHISSTYNTPFGVSGADFQTLPVGSSAAVEPLGLELVCRAPPGTSEGHWAWEATGAWNCSVENGGCEYLCNRSTNEPRCLCPRDMDLQADGRSCARPVVQSCNELCEHFCVSNAEVPGSYSCMCETGYQLAADGHRCEDVDDCKQGPNPCPQLCVNTKGGFECFCYDGYELVDGECVELLDPCFGSNCEFQCQPVSPTDYRCICAPGFAPKPDEPHKCEMFCNETSCPADCDPNSPTVCECPEGFILDEGSVCTDIDECSQGECFTSECRNFPGSYECICGPDTALAGQISKDCDPIPVREDTKEEEGSGEPPVSPTPGSPTGPPSARPVHS

Molecular Weight

85-90 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 .

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Thrombomodulin Protein, Mouse (HEK293, His)
Cat. No.:
HY-P72451
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