1. Recombinant Proteins
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TAM Receptor
  5. TYRO3
  6. TYRO3/DTK Protein, Mouse (HEK293, hFc)

TYRO3/DTK Protein, Mouse (HEK293, hFc)

Cat. No.: HY-P70043
COA Handling Instructions

TYRO3/DTK protein is a receptor tyrosine kinase that transduces signals through ligands such as TULP1 or GAS6 to regulate cell survival, migration and differentiation. Ligand binding induces TYRO3 dimerization and autophosphorylation, creating docking sites for downstream molecules. TYRO3/DTK Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived TYRO3/DTK protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TYRO3/DTK Protein, Mouse (HEK293, hFc) is 388 a.a., with molecular weight of 90-120 kDa.

For research use only. We do not sell to patients.

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Description

TYRO3/DTK protein is a receptor tyrosine kinase that transduces signals through ligands such as TULP1 or GAS6 to regulate cell survival, migration and differentiation. Ligand binding induces TYRO3 dimerization and autophosphorylation, creating docking sites for downstream molecules. TYRO3/DTK Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived TYRO3/DTK protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TYRO3/DTK Protein, Mouse (HEK293, hFc) is 388 a.a., with molecular weight of 90-120 kDa.

Background

The TYRO3/DTK protein, a receptor tyrosine kinase, transduces signals from the extracellular matrix by binding to various ligands, including TULP1 or GAS6. It plays a crucial role in regulating numerous physiological processes, encompassing cell survival, migration, and differentiation. Upon ligand binding at the cell surface, TYRO3 undergoes dimerization and autophosphorylation on its intracellular domain, creating docking sites for downstream signaling molecules. Upon activation, TYRO3 interacts with PIK3R1, enhancing PI3-kinase activity and activating the AKT survival pathway, leading to nuclear translocation of NF-kappa-B and up-regulation of transcription of NF-kappa-B-regulated genes. TYRO3 signaling is implicated in processes such as neuron protection from excitotoxic injury, platelet aggregation, and cytoskeleton reorganization. Additionally, TYRO3 plays a crucial role in inhibiting Toll-like receptors (TLRs)-mediated innate immune responses by activating STAT1, which selectively induces the production of suppressors of cytokine signaling SOCS1 and SOCS3.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

P55144 (A31-S418)

Gene ID

22174  [NCBI]

Molecular Construction
N-term
TYRO3 (A31-S418)
Accession # P55144
hFc
C-term
Synonyms
rMuTyrosine-protein kinase receptor TYRO3/DTK, Fc; Tyrosine-protein kinase receptor TYRO3; Etk2; tyro3; TK19-2; Tyrosine-protein kinase DTK; Tyrosine-protein kinase RSE; Tyrosine-protein kinase TIF
AA Sequence

AGLKLMGAPVKMTVSQGQPVKLNCSVEGMEDPDIHWMKDGTVVQNASQVSISISEHSWIGLLSLKSVERSDAGLYWCQVKDGEETKISQSVWLTVEGVPFFTVEPKDLAVPPNAPFQLSCEAVGPPEPVTIYWWRGLTKVGGPAPSPSVLNVTGVTQRTEFSCEARNIKGLATSRPAIVRLQAPPAAPFNTTVTTISSYNASVAWVPGADGLALLHSCTVQVAHAPGEWEALAVVVPVPPFTCLLRNLAPATNYSLRVRCANALGPSPYGDWVPFQTKGLAPARAPQNFHAIRTDSGLILEWEEVIPEDPGEGPLGPYKLSWVQENGTQDELMVEGTRANLTDWDPQKDLILRVCASNAIGDGPWSQPLVVSSHDHAGRQGPPHSRTS

Molecular Weight

90-120 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TYRO3/DTK Protein, Mouse (HEK293, hFc)
Cat. No.:
HY-P70043
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