UBE2B Protein, Human (HEK293, His)

The UBE2B protein is a key ubiquitination factor that acts as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing covalent attachment to proteins. UBE2B works in conjunction with the BRE1 (RNF20 and/or RNF40) E3 enzyme and plays a key role in transcriptional regulation by catalyzing the monoubiquitination of histone H2B at "Lys-120" (H2BK120ub1). UBE2B Protein, Human (HEK293, His) is the recombinant human-derived UBE2B protein, expressed by HEK293 , with C-6*His labeled tag.

UBE2B, a key player in the ubiquitination pathway, acts as an E2 ubiquitin-conjugating enzyme by accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to other proteins. Teaming up with the E3 enzyme BRE1 (RNF20 and/or RNF40), UBE2B assumes a pivotal role in transcriptional regulation, catalyzing the monoubiquitination of histone H2B at 'Lys-120' (H2BK120ub1). This modification serves as a distinctive tag for various epigenetic processes, including transcriptional activation, RNA polymerase II elongation, and telomeric silencing, and is essential for the formation of H3K4me and H3K79me. In addition to its histone-modifying functions, UBE2B exhibits versatile ubiquitin chain linkages, mediating 'Lys-11', 'Lys-48', and 'Lys-63'-linked polyubiquitination in vitro. Furthermore, UBE2B plays a crucial role in postreplication repair of UV-damaged DNA and collaborates with the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex, which mono-ubiquitinates DNA-associated PCNA on 'Lys-164'. The diverse functions of UBE2B suggest its potential involvement in neurite outgrowth.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

P63146 (M1-S152)

7320  [NCBI]

Full Length

MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Approximately 17 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Solution.

1.Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 6% trehalose, 0.03% Tween 20, pH 8.5.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.