UBE2B - ubiquitin conjugating enzyme E2 B Gene

Also Known as HR6B; UBC2; HHR6B; RAD6B; E2-17kDa

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 7320

About UBE2B

Cytogenetic location: 5q31.1 Genomic coordinates (GRCh38): 5:134,371,569-134,392,108 (from NCBI)

This gene has 8 transcripts (splice variants), 223 orthologues and 24 paralogues. Ubiquitous expression in adrenal (RPKM 31.0), heart (RPKM 26.8) and 25 other tissues.

Summary

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating Enzymes, or E1s, ubiquitin-conjugating Enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

UBE2B Products (2)

mRNA	Protein	Name
NM_003337.4	NP_003328.1	ubiquitin-conjugating enzyme E2 B
NM_003337.4	NP_003328.1	ubiquitin-conjugating enzyme E2 B

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables protein binding	IPI IPI: Inferred from physical interaction	12776193	GOA
enables ubiquitin conjugating enzyme activity	IDA IDA: Inferred from direct assay	38182926	GOA
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	10908344	GOA
enables ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	10373550	GOA
enables ubiquitin-protein transferase activity	IMP IMP: Inferred from mutant phenotype	18339854	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in DNA damage response	IDA IDA: Inferred from direct assay	14981545	GOA
involved in DNA repair	IGI IGI: Inferred from genetic interaction	1717990	GOA
involved in postreplication repair	IDA IDA: Inferred from direct assay	14981545	GOA
involved in proteasome-mediated ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	10373550	GOA
involved in protein K11-linked ubiquitination	IDA IDA: Inferred from direct assay	20061386	GOA
involved in protein K48-linked ubiquitination	IDA IDA: Inferred from direct assay	20061386	GOA
involved in protein K63-linked ubiquitination	IDA IDA: Inferred from direct assay	20061386	GOA
involved in protein autoubiquitination	IDA IDA: Inferred from direct assay	19123975	GOA
involved in protein stabilization	IMP IMP: Inferred from mutant phenotype	18339854	GOA
involved in protein ubiquitination	IDA IDA: Inferred from direct assay	10373550	GOA
involved in response to UV	IGI IGI: Inferred from genetic interaction	1717990	GOA
involved in response to xenobiotic stimulus	IDA IDA: Inferred from direct assay	14981545	GOA
involved in ubiquitin-dependent protein catabolic process	IDA IDA: Inferred from direct assay	10373550	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of HULC complex	IDA IDA: Inferred from direct assay	19410543	GOA
located in cytoplasm	IDA IDA: Inferred from direct assay	18339854	GOA
located in nucleus	IDA IDA: Inferred from direct assay	18339854	GOA
located in replication fork	IDA IDA: Inferred from direct assay	18363965	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

UBE2B Protein Structure

UQ_con

0
100
152 a.a.

Protein Preferred Names	Protein Names
ubiquitin-conjugating enzyme E2 B	E2 protein

UBE2B Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	UBE2B	P63146	RAD18	Homo sapiens	Q9NS91	X-Ray Diffraction	21422291
Intra	UBE2B	P63146	RAD18	Homo sapiens	Q9NS91	NMR	21422291
Intra	UBE2B	P63146	RAD18	Homo sapiens	Q9NS91	Anti Tag CoIP	33961781
Intra	UBE2B	P63146	RAD18	Homo sapiens	Q9NS91	SPR	21422291
Intra	UBE2B	P63146	RAD18	Homo sapiens	Q9NS91	Anti Tag CoIP	28514442
Intra	UBE2B	P63146	HERPUD2	Homo sapiens	Q9BSE4	Y2H Array	32296183
Intra	UBE2B	P63146	HERPUD2	Homo sapiens	Q9BSE4	Validated Y2H	32296183
Intra	UBE2B	P63146	HERPUD2	Homo sapiens	Q9BSE4	Y2H Prey Pooling	32296183
Intra	UBE2B	P63146	H2AC25	Homo sapiens	Q7L7L0	Pull Down	20080676
Intra	UBE2B	P63146	UBR1	Homo sapiens	Q8IWV7	NMR	21422291
Intra	UBE2B	P63146	UBR1	Homo sapiens	Q8IWV7	Y2H Pooling	16169070
Intra	UBE2B	P63146	DDIT4L	Homo sapiens	Q96D03	Validated Y2H	32296183
Intra	UBE2B	P63146	DDIT4L	Homo sapiens	Q96D03	Y2H Array	32296183
Intra	UBE2B	P63146	DDIT4L	Homo sapiens	Q96D03	Y2H Prey Pooling	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

Recombinant UBE2B Proteins

Cat. No.	Product Name	Accession	Purity
HY-P71398	UBE2B Protein, Human (HEK293, His)	P63146 (M1-S152)	≥ 95%, as determined by reducing SDS-PAGE.

Related Diseases

Diseases	Alias
Halothane Hepatitis	Hepatitis Halothane
Johanson-Blizzard Syndrome	JBS Nasal Alar Hypoplasia, Hypothyroidism, Pancreatic Achylia, And Congenital Deafness Nasal Alar Hypoplasia, Hypothyroidism, Pancreatic Achylia And Congenital Deafness Johanson Blizzard Syndrome

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS15337	UBE2B Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS20506	Ube2b Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS27014	Ube2b Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Macaca mulatta	UBE2B	VGNC	VGNC:78692
Canis familiaris	UBE2B	VGNC	VGNC:56072
Bos taurus	UBE2B	VGNC	VGNC:36577
Rattus norvegicus	UBE2B	RGD	RGD:708345
Mus musculus	UBE2B	MGD	MGI:102944
Felis catus	UBE2B	VGNC	VGNC:102356
Others	UBE2B	NCBI