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  6. Vaspin Protein, Human (HEK293, His)

Vaspin Protein, Human (HEK293, His)

Cat. No.: HY-P71300
COA Handling Instructions

Vaspin is an adipokine that regulates adipogenesis, metabolism, and inflammation by binding to the chemokine receptors CMKLR1 and CMKLR2. It mainly regulates adipocyte differentiation and affects lipid and glucose metabolism genes. Vaspin Protein, Human (HEK293, His) is the recombinant human-derived Vaspin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Vaspin Protein, Human (HEK293, His) is 394 a.a., with molecular weight of 50-60 kDa.

For research use only. We do not sell to patients.

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10 μg $107 In-stock
50 μg $300 In-stock
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Description

Vaspin is an adipokine that regulates adipogenesis, metabolism, and inflammation by binding to the chemokine receptors CMKLR1 and CMKLR2. It mainly regulates adipocyte differentiation and affects lipid and glucose metabolism genes. Vaspin Protein, Human (HEK293, His) is the recombinant human-derived Vaspin protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Vaspin Protein, Human (HEK293, His) is 394 a.a., with molecular weight of 50-60 kDa.

Background

Chemerin/RARRES2, an adipocyte-secreted protein (adipokine), orchestrates a multifaceted impact on adipogenesis, metabolism, and inflammation by activating the chemokine-like receptor 1 (CMKLR1) and also serving as a ligand for CMKLR2. While it can bind to C-C chemokine receptor-like 2 (CCRL2) with lower affinity, its primary role involves positively regulating adipocyte differentiation and influencing the expression of genes associated with lipid and glucose metabolism. Furthermore, Chemerin/RARRES2 plays a pivotal role in angiogenesis, essential for white adipose tissue expansion. As a pro-inflammatory adipokine, it amplifies the secretion of pro-inflammatory and prodiabetic adipokines, contributing to impaired metabolic function and systemic effects such as altered insulin sensitivity, disrupted glucose and lipid metabolism, and compromised vascular function in various tissues. Its enzymatic cleavage by different proteases can confer both pro- and anti-inflammatory properties. Acting as a chemotactic factor, it attracts leukocyte populations expressing CMKLR1, including plasmacytoid dendritic cells, myeloid dendritic cells, macrophages, and natural killer cells. Chemerin/RARRES2 also exhibits an anti-inflammatory role by inhibiting TNF/TNFA-induced VCAM1 expression and monocyte adhesion in vascular endothelial cells. This effect is mediated through the inhibition of NF-kappa-B and CRK/p38 activation, involving stimulation of AKT1/NOS3 signaling and nitric oxide production. This dual role in inflammation and metabolism suggests a potential link between chronic inflammation and obesity-related disorders, such as type 2 diabetes and cardiovascular disease. Additionally, Chemerin/RARRES2 demonstrates antimicrobial function in the skin.

Species

Human

Source

HEK293

Tag

C-10*His

Accession

Q8IW75 (L21-K414)

Gene ID
Molecular Construction
N-term
Vaspin (L21-K414)
Accession # Q8IW75
10*His
C-term
Synonyms
Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12
AA Sequence

LKPSFSPRNYKALSEVQGWKQRMAAKELARQNMDLGFKLLKKLAFYNPGRNIFLSPLSISTAFSMLCLGAQDSTLDEIKQGFNFRKMPEKDLHEGFHYIIHELTQKTQDLKLSIGNTLFIDQRLQPQRKFLEDAKNFYSAETILTNFQNLEMAQKQINDFISQKTHGKINNLIENIDPGTVMLLANYIFFRARWKHEFDPNVTKEEDFFLEKNSSVKVPMMFRSGIYQVGYDDKLSCTILEIPYQKNITAIFILPDEGKLKHLEKGLQVDTFSRWKTLLSRRVVDVSVPRLHMTGTFDLKKTLSYIGVSKIFEEHGDLTKIAPHRSLKVGEAVHKAELKMDERGTEGAAGTGAQTLPMETPLVVKIDKPYLLLIYSEKIPSVLFLGKIVNPIGK

Molecular Weight

50-60 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

Vaspin Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Vaspin Protein, Human (HEK293, His)
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HY-P71300
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