2. Metabolic Enzyme/Protease GPCR/G Protein Immunology/Inflammation
  3. Endogenous Metabolite Leukotriene Receptor Chemerin Receptor COX Lipoxygenase
  4. Resolvin E2

Resolvin E2  (Synonyms: (-)-Resolvin E2)

Cat. No.: HY-133159 Purity: 98.5%
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Resolvin E2 ((-)-Resolvin E2) is an endogenous lipid mediator produced from eicosapentaenoic acid (EPA) under the catalysis of 5-lipoxygenase (5-LOX), and its production increases in hypoxic environments. Resolvin E2 antagonizes BLT1, partially activates ChemR23, and promotes ubiquitin-proteasome-mediated degradation of COX-2. Resolvin E2 reduces the production of prostaglandin E2, blocks polymorphonuclear leukocyte infiltration, and promotes the resolution of airway inflammation. Resolvin E2 ameliorates lipopolysaccharide (LPS) (HY-D1056)-induced depressive-like behaviors. Resolvin E2 can be used in research related to depression, murine peritonitis, neonatal asthma, and other conditions.

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Resolvin E2

Resolvin E2 Chemical Structure

CAS No. : 865532-70-3

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5 μg (149.50 μM * 100 μL in Ethanol) In-stock

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Resolvin E2 Related Antibodies

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Human Endogenous Metabolite Microbial Metabolite

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LTB4 LTC4/CysLT2 LTD4/CysLT1 LTE4

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ChemR23/CMKLR1

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5-LOX 12-LOX 15-LOX

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Description

Resolvin E2 ((-)-Resolvin E2) is an endogenous lipid mediator produced from eicosapentaenoic acid (EPA) under the catalysis of 5-lipoxygenase (5-LOX), and its production increases in hypoxic environments. Resolvin E2 antagonizes BLT1, partially activates ChemR23, and promotes ubiquitin-proteasome-mediated degradation of COX-2. Resolvin E2 reduces the production of prostaglandin E2, blocks polymorphonuclear leukocyte infiltration, and promotes the resolution of airway inflammation. Resolvin E2 ameliorates lipopolysaccharide (LPS) (HY-D1056)-induced depressive-like behaviors. Resolvin E2 can be used in research related to depression, murine peritonitis, neonatal asthma, and other conditions[1][2][3][4].

In Vitro

Resolvin E2 (10 nM; 2-16 h) suppresses COX-2 protein expression in a time-dependent manner (significantly at 4-16 h) without altering COX-1 levels in human macrophage-like U937 cells[3].
Resolvin E2 (10 nM; 4-16 h) significantly reduces PGE2 production in human macrophage-like U937 cells[3].
Resolvin E2 (10 nM; 8 h) promotes COX-2 protein degradation via the ubiquitin-proteasome system in human macrophage-like U937 cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Resolvin E2 Related Antibodies

Western Blot Analysis[3]

Cell Line: human macrophage-like U937 cells (differentiated with phorbol 12-myristate 13-acetate)
Concentration: 10 nM
Incubation Time: 2 h, 4 h, 8 h, 16 h
Result: Did not alter COX-1 protein levels at any time point tested.
Suppressed COX-2 protein expression in a time-dependent manner, with statistically significant suppression observed at 4 h, 8 h, and 16 h compared to the 0 h control.

ELISA Assay[3]

Cell Line: human macrophage-like U937 cells (differentiated with phorbol 12-myristate 13-acetate)
Concentration: 10 nM
Incubation Time: 2 h, 4 h, 8 h, 16 h
Result: Reduced PGE2 production in a time-dependent manner, with statistically significant suppression observed at 4 h, 8 h, and 16 h compared to the 0 h control.

Real Time qPCR[3]

Cell Line: human macrophage-like U937 cells (differentiated with phorbol 12-myristate 13-acetate)
Concentration: 10 nM
Incubation Time: 2 h, 4 h, 8 h, 16 h
Result: Did not suppress COX-2 mRNA expression at any time point tested, with no statistically significant differences compared to the 0 h control.

Western Blot Analysis[3]

Cell Line: human macrophage-like U937 cells (differentiated with phorbol 12-myristate 13-acetate)
Concentration: 10 nM (Resolvin E2); 0.3 μM (MG132 pretreatment)
Incubation Time: 8 h (Resolvin E2); 30 min (MG132 pretreatment)
Result: Suppressed COX-2 protein expression when administered alone.
Had its COX-2 protein expression suppression abrogated by pretreatment with 0.3 μM MG132, which restored COX-2 protein levels to a similar level as the untreated control.
In Vivo

Resolvin E2 (1-10 ng; intracerebroventricular injection; single infusion) exerts dose-dependent antidepressant effects in LPS-induced depressive-like behaviors in mice, with significant efficacy observed at the 10 ng intracerebroventricular injection dose in the forced swim test[1].
Resolvin E2 (1-100 ng; intravenous injection, intraperitoneal injection; single administration) potently reduces human neutrophils (PMN) infiltration in zymosan-induced murine peritonitis[2].
Resolvin E2 (300 ng/mouse; intranasal administration; once daily for 3 consecutive days) alleviates neonatal asthma in high-risk pups by reducing airway eosinophilia, pulmonary tissue infiltration, and allergenic cytokine levels[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c (male, 11-12 weeks old, LPS-induced depression model)[1]
Dosage: 1 ng; 10 ng
Administration: i.c.v.; single infusion
Result: Dose-dependently decreased LPS-induced increases in immobility time in the tail suspension test.
Significantly suppressed LPS-induced increases in immobility time in the forced swim test at the 10 ng i.c.v. dose.
Had no effect on immobility time in saline-injected control mice in either tail suspension or forced swim tests.
Did not affect locomotor activity or anxiety-like behavior (time spent in the center of an open field chamber) in either LPS-challenged or saline-injected mice.
Animal Model: FVB (male, 6-8 weeks old, peritonitis induced by intraperitoneal injection of 1 mg zymosan A in 1 mL saline)[2]
Dosage: 1 ng (i.v., side-by-side comparison); 10 ng (i.v., side-by-side comparison); 100 ng (i.v., side-by-side comparison); 10 ng (i.v., route comparison); 10 ng (i.p.)
Administration: i.v.; single dose; i.p.; single dose
Result: Reduced PMN infiltration by 11.3% compared to controls.
Reduced PMN infiltration by 17.7% compared to controls.
Reduced PMN infiltration by 33.7% compared to controls.
Reduced PMN infiltration by 47.6% compared to controls.
Reduced PMN infiltration by 34.5% compared to controls.
Combined with 10 ng Resolvin E1 (i.v.) reduced PMN infiltration additively.
Showed potency not significantly different from Resolvin E1 at any tested dose.
Animal Model: BALB/c (neonatal, offspring of time-pregnant E13 BALB/c dams; neonatal asthma model induced by maternal gestational exposure to CAPs/DEPs (concentrated urban air particles and diesel exhaust particles) at E14-E20, followed by neonatal OVA (HY-W250978) sensitization and challenge)[4]
Dosage: 300 ng/mouse
Administration: i.n.; once daily; 3 days
Result: Reduced BAL eosinophil counts by more than half and eosinophil percentages by ~20% in offspring of CAP- or DEP-exposed dams compared to untreated at-risk pups.
Decreased BAL IL-5 levels in offspring of DEP-exposed dams.
Decreased BAL IL-13 levels in offspring of DEP-exposed dams.
Decreased serum IL-5 levels in offspring of CAP-exposed dams.
Ameliorated lung tissue inflammatory infiltration compared to untreated at-risk pups.
Showed no effect in offspring of PBS-exposed control dams.
Molecular Weight

334.45

Formula

C20H30O4
CAS No.
Appearance

Liquid

Color

Colorless to light yellow

SMILES

CC[C@@H](O)/C=C/C=C\C/C=C\C/C=C\C=C\[C@@H](O)CCCC(O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Solution, -20°C, 2 years
Purity & Documentation

Purity: 98.5%

SDS (393 KB)

COA (239 KB)

Handling Instructions (2659 KB)
References
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Resolvin E2 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Resolvin E2865532-70-3(-)-Resolvin E2Resolvin E 2Resolvin E-2Endogenous MetaboliteLeukotriene ReceptorChemerin ReceptorCOXLipoxygenaseCyclooxygenaseLOXCOX-1murine modelsBLT1ChemR23neonatal asthmaeicosapentaenoic acidcyclooxygenase-2human macrophage-like U937 cellsubiquitin-proteasome systemhuman neutrophilsInhibitorinhibitorinhibit

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