2. Epigenetics
    PI3K/Akt/mTOR
    Stem Cell/Wnt
    JAK/STAT Signaling
    Autophagy
  3. AMPK
    STAT
    Autophagy
  4. RSVA405

RSVA405 

Cat. No.: HY-103238 Purity: 99.58%
COA Handling Instructions

RSVA405 is a potent, orally active activator of AMPK, with an EC50 of 1 μM. RSVA405 facilitates CaMKKβ-dependent activation of AMPK, inhibits mTOR, and promotes autophagy to increase Aβ degradation. RSVA405 has anti-inflammatory effects through the inhibition of STAT3 function. RSVA405 can also be used for the research of obesity.

For research use only. We do not sell to patients.

RSVA405 Chemical Structure

RSVA405 Chemical Structure

CAS No. : 140405-36-3

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Solution
10 mM * 1 mL in DMSO USD 121 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 121 In-stock
Solid
5 mg USD 110 In-stock
10 mg USD 180 In-stock
25 mg USD 400 In-stock
50 mg USD 700 In-stock
100 mg USD 1150 In-stock
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Based on 1 publication(s) in Google Scholar

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  • Biological Activity

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  • References

  • Customer Review

Description

RSVA405 is a potent, orally active activator of AMPK, with an EC50 of 1 μM. RSVA405 facilitates CaMKKβ-dependent activation of AMPK, inhibits mTOR, and promotes autophagy to increase Aβ degradation. RSVA405 has anti-inflammatory effects through the inhibition of STAT3 function. RSVA405 can also be used for the research of obesity[1][2][3][4].

IC50 & Target[2]

AMPK

1 μM (EC50, in cell-based assays)

In Vitro

RSVA405 (0.2-2 μM; 24 h) inhibits adipocyte differentiation[2].
RSVA405 (0.2-2 μM; 24 h) significantly inhibits the expression of peroxisome proliferator-activated receptor (PPAR)-γ, fatty acid synthase (FAS) and fatty acid binding protein 4 (aP2) in 3T3-L1 cells[2].
RSVA405 (1-3 μM; 16 h) inhibits LPS-induced STAT3 activity, intracellular signaling, and cytokine response in activated RAW 264.7 macrophages[3].
RSVA405 (1-3 μM; 24 h) inhibits mTOR, induces autophagy, and facilitates the lysosomal degradation of Aβ, with an EC50 of ∼1 μM in APP-HEK293 cells[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: 3T3-L1 preadipocytes
Concentration: 0.2, 0.5, 1, 2 μM
Incubation Time: 24 h
Result: Increased the phosphorylation of AMPK and its substrate acetyl-CoA carboxylase (ACC).
Inhibited the accumulation of lipid droplets in a dose-dependent manner, with an IC50 of 0.5 μM.
In Vivo

RSVA405 (3 mg/kg; i.p.) attenuates renal injury and protects renal function after ischemia-reperfusion (I/R) in rats[1].
RSVA405 (20-100 mg/kg/d; p.o. for 11 weeks) significantly reduces the body weight gain of mice fed a high-fat diet[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (300-350 g) are induced I/R injury[1]
Dosage: 3 mg/kg
Administration: I.p. one hour before inducing I/R injury
Result: Decreased the levels of creatinine and blood urea nitrogen (BUN), by 35.8% and 44.3% in serum, respectively.
Decreased the levels of aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) by 33.0% and 59.8% in serum, respectively.
Molecular Weight

312.37

Appearance

Solid

Formula

C17H20N4O2
CAS No.
SMILES

O=C(C1=CC=NC=C1)N/N=C/C2=CC=C(N(CC)CC)C=C2O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (400.17 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2013 mL 16.0067 mL 32.0133 mL
5 mM 0.6403 mL 3.2013 mL 6.4027 mL
10 mM 0.3201 mL 1.6007 mL 3.2013 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.58%

COA (246 KB) HNMR (257 KB) LCMS (257 KB)

Handling Instructions (2659 KB)
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

RSVA405140405-36-3RSVA 405RSVA-405AMPKSTATAutophagyAMP-activated protein kinaseAMP-ActivatedProteinKinaseanti-inflammatorySTAT3obesityAlzheimer'sdiseaseI/RInhibitorinhibitorinhibit

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