2. Epigenetics PI3K/Akt/mTOR
  3. AMPK
  4. BAY-3827

BAY-3827, a chemical probe, is a potent and selective AMPK inhibitor with IC50 values of 1.4 nM at low (10 μM ATP concentration) and 15 nM at high (2 mM ATP concentration). BAY-3827 shows over 500-fold selectivity for most of the 331 kinases. BAY-3827 prevents phosphorylation of acetyl-CoA carboxylase 1 and shows strongest anti-proliferative activity in androgen-dependent prostate cancer cell lines.

For research use only. We do not sell to patients.

CAS No. : 2377576-35-5

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Customer Review

Based on 15 publication(s) in Google Scholar

BAY-3827 Related Antibodies

Top Publications Citing Use of Products

    BAY-3827 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec 15.

    BAY-3827 at 10 mg/kg for a duration of 4 weeks. Distinctive micro-CT visuals showcasing both longitudinal and cross-sectional views of the distal femur segments.

    BAY-3827 purchased from MedChemExpress. Usage Cited in: J Pharm Anal. 2025 Dec 15.

    BAY-3827 was administered at 10 mg/kg for a duration of 4 weeks. Representative images of hematoxylin and eosin (H&E) staining and Masson's staining of the metaphyseal tissue sections of the thigh were presented.

    BAY-3827 purchased from MedChemExpress. Usage Cited in: J Agric Food Chem. 2025 Dec 22.  [Abstract]

    ELISA quantification of IL-1β in BMDM supernatants following LPS + nigericin stimulation with or without Api, the AMPK agonist AICAR, or the AMPK inhibitor BAY-3827 (500 nM) was performed.

    BAY-3827 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Oct 16;26(20):10098.  [Abstract]

    HCFs were incubated with the AMPK inhibitor BAY-3827 (500 nM) for 1 h prior to stimulation with DMSO (vehicle) or indicated concentrations of SGLT2i for 3 h.

    BAY-3827 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2025 Oct 16;26(20):10098.  [Abstract]

    HCFs were incubated with the AMPK inhibitor BAY-3827 (500 nM) for 1 h prior to stimulation with DMSO (vehicle) or indicated concentrations of SGLT2i for 72 h.

    View All AMPK Isoform Specific Products:

    View All Isoforms
    NUAK1 NUAK2 AMPK AMPK α1β1γ1 AMPK α2β1γ1 AMPK α1β2γ1 AMPK α2β2γ1 BRSK1 BRSK2 HUNK AMPKα

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    BAY-3827, a chemical probe, is a potent and selective AMPK inhibitor with IC50 values of 1.4 nM at low (10 μM ATP concentration) and 15 nM at high (2 mM ATP concentration). BAY-3827 shows over 500-fold selectivity for most of the 331 kinases. BAY-3827 prevents phosphorylation of acetyl-CoA carboxylase 1 and shows strongest anti-proliferative activity in androgen-dependent prostate cancer cell lines[1].

    IC50 & Target

    IC50: 1.4 nM (AMPK kinase,10 µM ATP), 15 nM (AMPK kinase, 2 mM ATP), 1324 nM (Aurora A), 124 nM (Flt3), 788 nM (c-Met), 36 nM (Rsk4)[1]
    In Vitro

    BAY-3827 (0-200 μM) inhibits AMPK kinase activity with IC50 values of 1.4 nM at low, 10 μM ATP concentration and 15 nM at high, 2 mM ATP concentration[1].
    BAY-3827 (0-200 μM) inhibits Aurora A, Flt3, c-Met and Rsk4 with IC50 values of 1324, 124, 788 and 36 nM , respectively with 10 μM ATP concentration[1].
    BAY-3827 (overnight) strongly reduces ACC1 Ser79 phosphorylation in LNCaP and VCaP cells, and shows a lesser extent in IMR-32 and especially in Colo320 cells[1].
    BAY-3827 (0-10 nM; 6 d) shows strong inhibitory effects to LNCaP and VCaP cells[1].
    BAY-3827 (1 and 5 μM; 24 and 48 h) represses LIPE gene expression, reduces serine/threonine kinase AKT3 and blocks the expression of several genes from the mitochondrial carnitine palmitoyltransferase (CPT) family which is involved in acyl carnitine formation in VCaP cells[1].
    BAY-3827 (5 μM; 2-4 d) significantly increases the formation of lipid droplets in comparison to androgen treatment only[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    BAY-3827 Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: LNCaP, VCaP, 22Rv1, C4-2B, PC-3 and DU-145 prostate cancer cell lines
    Concentration: 0-10 nM
    Incubation Time: 6 d
    Result: Showed strong inhibitory effects for LNCaP and VCaP cells, two prostate cancer cell lines with IC50 values of 0.28 and 1.71 nM, respectily. Inhibited proliferation of 22Rv1 cells with an IC50 value of 5.55 nM.
    Molecular Weight

    468.53

    Formula

    C27H25FN6O
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    CCC1=CC=CC=C1C(NC2=NNC3=C2C=C(C4C(C#N)=C(C)N(C)C(C)=C4C#N)C(F)=C3C)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (53.36 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1343 mL 10.6717 mL 21.3434 mL
    5 mM 0.4269 mL 2.1343 mL 4.2687 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Purity & Documentation

    Purity: 99.72%

    SDS (252 KB)

    COA (258 KB) HNMR (304 KB) RP-HPLC (265 KB) LCMS (103 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1343 mL 10.6717 mL 21.3434 mL 53.3584 mL
    5 mM 0.4269 mL 2.1343 mL 4.2687 mL 10.6717 mL
    10 mM 0.2134 mL 1.0672 mL 2.1343 mL 5.3358 mL
    15 mM 0.1423 mL 0.7114 mL 1.4229 mL 3.5572 mL
    20 mM 0.1067 mL 0.5336 mL 1.0672 mL 2.6679 mL
    25 mM 0.0854 mL 0.4269 mL 0.8537 mL 2.1343 mL
    30 mM 0.0711 mL 0.3557 mL 0.7114 mL 1.7786 mL
    40 mM 0.0534 mL 0.2668 mL 0.5336 mL 1.3340 mL
    50 mM 0.0427 mL 0.2134 mL 0.4269 mL 1.0672 mL
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    BAY-3827 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    BAY-38272377576-35-5BAY3827BAY 3827AMPKAMP-activated protein kinaseATPAurora AFlt3c-MetRsk4phosphorylationLNCaPVCaPLIPEAKT3lipid dropletInhibitorinhibitorinhibit

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