Search Result
Results for "
Fluorescence assay
" in MedChemExpress (MCE) Product Catalog:
2
Biochemical Assay Reagents
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Product Name |
Target |
Research Areas |
Chemical Structure |
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- HY-D2908
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Fluorescent Dye
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Others
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SYTO9 is a green fluorescent nucleic acid dye (Ex = 485 nm, Em = 498 nm (DNA)/501 nm (RNA)). SYTO9 can penetrate the intact cell membrane of viable bacteria. When the cell membrane is damaged (cell death), its rate of entry into cells increases, and it competes with propidium iodide (PI) (HY-D0815), another membrane-impermeable dye, for nucleic acid binding sites. SYTO9 is widely used in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology for bacterial counting, viability assessment, and cell imaging .
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- HY-D0952
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Parasite
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Others
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Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays .
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- HY-15943
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6-TAMRA
1 Publications Verification
6-Carboxytetramethylrhodamine
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Fluorescent Dye
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Others
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6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays .
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- HY-15941
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Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers
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Fluorescent Dye
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Others
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5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm .
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- HY-101027
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- HY-175686
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Ligands for E3 Ligase
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Cancer
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E3 ligase Ligand 67 (Compound 5) is a STUB1/CHIP ligand with a Kd of 8.9 μM for CHIP in fluorescence polarization assay. E3 ligase Ligand 67 is an E3 ligase ligand that can be used for synthesis of PROTACs .
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- HY-163385
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DNA Methyltransferase
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Cancer
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ALKBH1-IN-1 (Compound 13h) is a selective ALKBH1 inhibitor, with an IC50 of 0.026 μM and 1.39 μM in the fluorescence polarization and enzyme activity assay, respectively. ALKBH1-IN-1 can modulate the level of DNA 6mA modifications. ALKBH1-IN-1 can be used to study the functions of ALKBH1 and DNA 6mA .
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- HY-152231
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Fluorescent Dye
Ligands for E3 Ligase
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Others
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BODIPY FL thalidomide is a high-affinity and selective human cereblon (CRBN)-targeting fluorescent probe with a Kd of 3.6 nM. BODIPY FL thalidomide enables use as a probe in time-resolved fluorescence resonance energy transfer (TR-FRET) binding assays for cereblon ligands. BODIPY FL thalidomide supports development of highly sensitive, selective, stable cereblon TR-FRET binding assays (Ex/Em = 502/510 nm) .
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- HY-117468
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Fluorescent Dye
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Others
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Lissamine rhodamine B is a red-fluorescent dye, it is a derivative of rhodamine. Lissamine rhodamine B can be used as a fluorescent probe to develop competitive aptamer fluorescence anisotropy/polarization (FA/FP) assays .
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- HY-120635
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PD-1/PD-L1
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Inflammation/Immunology
Cancer
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BMS-1001 is an orally active human PD-L1/PD-1 immune checkpoint inhibitor. BMS-1001 exhibits low-toxicity in cells. The IC50 value of BMS-1001 in a homogeneous time-resolved fluorescence (HTRF) binding assay is 2.25 nM .
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- HY-101027A
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- HY-153232
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Fluorescent Dye
Liposome
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Others
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eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .
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- HY-133705
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HSP
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Others
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Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90 (Ex/Em = 488/515 nm) .
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- HY-131009
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PARP
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Others
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Fluorescein-NAD+ is an alternative to radiolabeled NAD and a substrate for ADP-ribosylation. Fluorescein-NAD+ can be used in PARP assays by fluorescence microscopy. Extinction Coefficient: 262 nm.
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- HY-131475
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Biochemical Assay Reagents
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Others
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Naphthol AS-BI phosphate is a substrate for acid and alkaline phosphatase and can be used in fluorescence assays. Naphthol AS-BI phosphate is a kind of biological materials or organic compounds that are widely used in life science research .
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- HY-134977
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Ras
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Cancer
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Atrovastatin-PEG3-FITC (compound S31) is a KRAS-PDEδ interaction inhibitor. Atrovastatin-PEG3-FITC acts as a ligand in fluorescence anisotropy assay .
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- HY-W755033
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- HY-131618
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Bz-Gly-Arg
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Amino Acid Derivatives
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Others
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Hippuryl-Arg (Bz-Gly-Arg) is a substrate used for fluorescence detection and is commonly used in biochemical studies, especially in studying enzyme activity. The phenylalanine portion of Hippuryl-Arg is connected by an aromatic side chain, which allows it to be detected under ultraviolet light. Hippuryl-Arg is used to detect enzymes that recognize and cut peptide bonds containing arginine .
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- HY-P2185A
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MMP
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Others
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NFF-3 TFA, the peptide, is a selective MMP substrate. NFF-3 TFA selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 TFA is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 TFA with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .
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- HY-D2415
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Fluorescent Dye
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Others
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BODIPY-FL staurosporine (Compound 8a) is a fluorescence probe based on staurosporine, exhibiting high specificity towards tyrosine kinases (TK) and tyrosine kinase-like (TKL) family kinases. BODIPY-FL staurosporine has the potential to develop binding assays for kinases that are not recommended for use with Kinase Tracer 236, such as PIM3, CDC42BPG, MAP2K7, TXK, and SIK3. BODIPY-FL staurosporine can be a powerful tool for analyzing kinase selectivity in kinase drug discovery .
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- HY-D0142
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Fluorescent Dye
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Others
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Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium is a fluorescent dye and pH indicator, also as a ligand of multifunctional metal-organic framework. Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium has been used to detect CO2 release. Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium acts as a water-soluble fluorescent dye for fluorescence quenching assays monitoring gramicidin ion-channel activity .
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- HY-130433
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Fluorescent Dye
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Others
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NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases .
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- HY-D1190
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RAR/RXR
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Others
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DC271 is a RAR agonist and synthetic retinoid that binds to the retinoid-binding site of cellular retinoic acid-binding protein II (CRBP-II). DC271 exhibits solvatochromic fluorescence properties: it produces intense blue-shifted emission in nonpolar environments and weak red-shifted emission in polar environments, and its severe fluorescence quenching in aqueous solutions can be reversed by embedding in the hydrophobic retinoid-binding protein pocket. DC271 enables direct detection of the binding between unlabeled compounds and related retinoid-binding proteins via fluorescence competition assays (Ex/Em = 355 nm/460 nm) .
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- HY-P4331
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Fluorescent Dye
Ser/Thr Protease
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Cardiovascular Disease
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Boc-Gln-Gly-Arg-AMC is a fluorogenic substrate for kallikrein (PKa), coagulation factor XIIa (FXIIa) and coagulation factor XIa (FXIa). Boc-Gln-Gly-Arg-AMC enables fluorescence-based activity assays for PKa, FXIIa and FXIa .
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- HY-107864
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Tetraiodofluorescein
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Fluorescent Dye
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Infection
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Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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- HY-139362
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Phosphodiesterase (PDE)
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Cancer
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Enpp-1-IN-2 (Compound C) is a potent ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1) inhibitor with IC50 values of 0.26, 0.48 and 2.0 μM evaluated by means of TG-mAMP, pNP-TMP, and ATP assays, respectively. TG (Tokyo Green)-mAMP: a newly synthesized sensitive ENPP1 fluorescence probe .
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- HY-P5485
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Bacterial
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Others
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Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .
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- HY-18717
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Isocitrate Dehydrogenase (IDH)
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Cancer
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Mutant IDH1-IN-2 is a inhibitor of mutant Isocitrate dehydrogenase (IDH) proteins, with IC50 of in LS-MS biochemical assay, IC50 of 16.6 nM in Fluorescence biochemical assay.
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- HY-P4725
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Fluorescent Dye
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Others
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Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2 is a fluorogenic substrate, that can be used for the fluorescence screening assay .
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- HY-12791
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- HY-157558
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8SGE
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Ligands for E3 Ligase
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Others
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KDRLKZ-1 is a KLHDC2 ligand (Kd = 0.36 μM), with IC50s of 0.21 μM and 0.31 μM in alphaLISA and time-resolved fluorescence resonance energy transfer (TR-FRET) displacement assays .
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- HY-150175
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Fluorescent Dye
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Inflammation/Immunology
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HKSOX-1 and its derivatives (HKSOX-1r and HKSOX-1m) are novel fluorescent probes designed for highly sensitive and selective detection of the superoxide anion radical (O2 •−) in cellular environments. These probes utilize an aryl trifluoromethanesulfonate group that undergoes O2 •−-mediated cleavage, releasing a free phenol and emitting fluorescence. They demonstrate excellent specificity and sensitivity across various pH ranges, withstand interference from strong oxidants and reductants typical in cellular contexts. HKSOX-1r, optimized for cellular retention, has been effectively employed in diverse assays including confocal imaging, flow cytometry, and zebrafish embryo studies, highlighting its utility in investigating O2 •− roles in inflammation, mitochondrial stress, and other physiological processes .
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- HY-175250
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TNF Receptor
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Inflammation/Immunology
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TNF-α-IN-25 is an orally active TNF-α inhibitor. TNF-α-IN-25 shows Fluorescence Polarization (FP) assay IC50 of 103 nM in FP binding assays and L929 assay IC50 of 505 nM in cell-based assays. TNF-α-IN-25 inhibits paw swelling in the glucose-6-phosphate isomerase (GPI) arthritis model. TNF-α-IN-25 can be used for the study of arthritis .
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- HY-W015462
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4-(1H-Imidazol-1-yl)aniline
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HSV
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Infection
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LANA-IN-1 (compound 8) is an N-acetyl-derived LANA inhibitor with antiviral activity against herpesvirus. In fluorescence assays using the LANA probe LBS2, 1 mM LANA-IN-1 shows an inhibition rate of 32%. LANA-IN-1 may inhibit the interaction between LANA and the viral genome .
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- HY-120647
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PD-1/PD-L1
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Inflammation/Immunology
Cancer
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BMS-1001 is an orally active human PD-L1/PD-1 immune checkpoint inhibitor. BMS-1001 exhibits low-toxicity in cells. The IC50 value of BMS-1001 in a homogeneous time-resolved fluorescence (HTRF) binding assay is 2.25 nM .
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- HY-12577
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PARP
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Cancer
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TNKS1/2-IN-1 (Example 4) is a TNKS1 and TNKS2 inhibitor, with pIC50 values of 8.2 or 7.3 against TNKS1, and 7.1 or 7.7 against TNKS2. TNKS1/2-IN-1 inhibits the enzymatic activities of TNKS1 and TNKS2. TNKS1/2-IN-1 is applicable for cancer research .
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- HY-118210
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FAAH
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Metabolic Disease
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PHOP is a fatty acid amide hydrolase (FAAH) inhibitor used to assess inhibitory activity in a fluorometric assay. PHOP can determine FAAH activity by measuring the amount of 4-pyridin-1-ylbutyric acid released by the enzyme in rat brain microsomes. PHOP demonstrates potential as a FAAH inhibitor and can directly measure FAAH activity by reversed-phase HPLC and fluorescence detection, providing a basis for the development of new inhibitors.
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- HY-P2185
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MMP
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Others
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NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .
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- HY-D2060
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Fluorescent Dye
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Others
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ATTO 740 is a near-infrared dye (Ex/Em: 740/764 nm). ATTO 740 can be conjugated with the Cetuximab antibody (HY-P9905) .
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- HY-D1678
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Fluorescent Dye
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Others
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5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .
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- HY-131009A
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PARP
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Others
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Fluorescein-NAD+ sodium is an alternative to radiolabeled NAD and a substrate for ADP-ribosylation. Fluorescein-NAD+ sodium can be used in PARP assays by fluorescence microscopy. Extinction Coefficient: 262 nm.
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- HY-P10344
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Fluorescent Dye
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Infection
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MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .
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- HY-159601
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PD-1/PD-L1
E1/E2/E3 Enzyme
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Cancer
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CSN5-IN-1 (compound Ac-11) is an inhibitor of CSN5, with IC50 values of 12.56 μM and 19 μM as measured by FP assay and fluorescence assay, respectively. CSN5-IN-1 can also downregulate the expression of PD-L1 and upregulate the expression of NEDD8-Cul1 in cells .
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- HY-153114
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FXR
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Inflammation/Immunology
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HEC96719 is a selective and orally active tricyclic farnesoid X receptor (FXR) agonist with EC50 values of 1.37 and 1.55 nM by time-resolved fluorescence energy transfer (TR-FRET) and luciferase reporter assays, respectively. HEC96719 significantly improves non-alcoholic steatohepatitis (NASH) and liver fibrosis with favorable tissue distribution in liver and intestine. HEC96719 can be used for the research of non-alcoholic steatohepatitis .
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- HY-169415
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WDR5
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Others
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DBL-6-13 is an inhibitor for WDR5 with a moderate binding affinity of 6.8 μM (microscale thermophoresis assay) and 9.1 μM (fluorescence polarization assay) .
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- HY-D0038
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Fluorescent Dye
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Inflammation/Immunology
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BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay .
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- HY-W800831
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Fluorescent Dye
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Others
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Tetra-sulfo-Cy7 DBCO is a bright and photostable near-IR probe. The Cy7 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
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- HY-D2761
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Fluorescent Dye
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Others
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DY-680-NHS ester is an amine reactive hydrophilic fluorochrome. It can be conjugated to an antibody with higher D/P ratio without causing fluorescence quenching and conjugate precipitation, and it is widely used in applications including western blotting, microscopy, flow cytometry, and cell-based assays. The spectrum has an excitation of 690nm and emission at 709nm.
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- HY-117983
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Amyloid-β
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Neurological Disease
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RU-505 is an effective β-amyloid (Aβ)-fibrinogen interaction inhibitor with IC50s of 5.00 and 2.72 μM in fluorescence polarization (FP) and AlphaLISA assays, respectively. RU-505 is highly permeable to the BBB. RU-505 reduces cerebral amyloid angiopathy (CAA). RU-505 can be used for the research of Alzheimer’s disease (AD) .
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- HY-P5415
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HIV
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Others
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DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-Kd protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)
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- HY-163844
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PD-1/PD-L1
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Inflammation/Immunology
Cancer
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PD-1/PD-L1-IN-49 (compound 1c) is a potent inhibitor of PD-1/PD-L1, with the IC50 of 77 nM. PD-1/PD-L1-IN-49 activates Jurkat T cells, reflecting successful blockade of the PD-1/PD-L1 immune checkpoint .
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- HY-124987
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Endogenous Metabolite
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Inflammation/Immunology
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MRS4458 is a P2Y14 receptor antagonist with anti-inflammatory activity. MRS4458 mediates inflammatory activity by activating the motility of neutrophils. The design of MRS4458 is based on the interaction with the P2Y14 receptor and optimized by molecular dynamic simulation. MRS4458 performs well in terms of shape and complementarity with the receptor. The prediction that the 5-phenyl group of MRS4458 is substituted with thiophene is generally consistent with empirical results. The biological activity of MRS4458 was verified by fluorescence assay, showing its potent antagonistic effect .
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- HY-121364
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Histamine Receptor
Fluorescent Dye
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Others
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Bodilisant is a histamine H3 receptor (hH3R) ligand and imaging/labeling agent, with a Ki value of 6.51 nM for hH3R. Bodilisant binds to hH3R to produce strong green fluorescence, localizes to the extracellular membrane without internalization, and generates clear, displaceable fluorescent labeling of hH3R in native human brain tissues. Bodilisant serves as a pharmacological tool to visualize the distribution of hH3R via fluorescence confocal laser scanning microscopy .
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- HY-107864A
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Tetraiodofluorescein aluminum
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Fluorescent Dye
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Infection
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Erythrosine B aluminum is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B aluminum can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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- HY-101027R
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- HY-101027AR
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- HY-P11481
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Fluorescent Dye
E1/E2/E3 Enzyme
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Others
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FITC-Acp-RpYTPEP-NH2 is a potent fluorescent tracer. FITC-Acp-RpYTPEP-NH2 shows a binding affinity (Kd = 0.07 μM) for the Cbl-b SH2 domain. Fluorescence polarization assay based on FITC-Acp-RpYTPEP-NH2 can be for evaluating Cbl-b SH2 domain inhibitors .
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- HY-107864R
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Tetraiodofluorescein (Standard)
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Reference Standards
Fluorescent Dye
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Infection
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Erythrosine B free acid (Standard) is the analytical standard of Erythrosine B (free acid) (HY-107864). This product is intended for research and analytical applications. Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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- HY-W043574
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Bcl-2 Family
Drug Derivative
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Cancer
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6-Ethyl-7-hydroxy-4-methyl-2H-chromen-2-one (Compound 32), a derivative of Coumarin (HY-N0709), acts as a Mcl-1 inhibitor with a Ki of 5.47 μM and an IC50 of 30.88 μM. 6-Ethyl-7-hydroxy-4-methyl-2H-chromen-2-one is applicable for cancer research .
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- HY-W727480
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Insecticide
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Infection
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Tetrachlorantraniliprole is an anthranilic diamide insecticide that shows no significant affinity to the key odorant-binding proteins (DhelOBP4/DhelOBP21) of Dastarcus helophoroides .
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- HY-183293
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E1/E2/E3 Enzyme
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Others
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Cbl-b-IN-32 is a Cbl-b inhibitor with an IC50 of 6.83 μM. Cbl-b-IN-32 binds to the TKB-LH interface, stabilizes the inactive conformation of Cbl-b, engages Tyr363 via hydrogen bonds and water bridges, and forms hydrogen bonds with Phe261 and Phe263 .
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| Cat. No. |
Product Name |
Type |
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- HY-D2908
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Fluorescent Dyes
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SYTO9 is a green fluorescent nucleic acid dye (Ex = 485 nm, Em = 498 nm (DNA)/501 nm (RNA)). SYTO9 can penetrate the intact cell membrane of viable bacteria. When the cell membrane is damaged (cell death), its rate of entry into cells increases, and it competes with propidium iodide (PI) (HY-D0815), another membrane-impermeable dye, for nucleic acid binding sites. SYTO9 is widely used in fluorescence microscopy, flow cytometry, and fluorometric assays in microbiology for bacterial counting, viability assessment, and cell imaging .
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- HY-D0952
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Fluorescent Dyes
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Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays .
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- HY-15943
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6-TAMRA
1 Publications Verification
6-Carboxytetramethylrhodamine
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Fluorescent Dyes
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6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays .
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- HY-15941
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Fluorescein 5(6)-isothiocyanate; Fluorescein isothiocyanate 5- and 6- isomers
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Fluorescent Dyes
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5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm .
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- HY-133705
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Fluorescent Dyes
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Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90 (Ex/Em = 488/515 nm) .
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- HY-D2415
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Fluorescent Dyes
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BODIPY-FL staurosporine (Compound 8a) is a fluorescence probe based on staurosporine, exhibiting high specificity towards tyrosine kinases (TK) and tyrosine kinase-like (TKL) family kinases. BODIPY-FL staurosporine has the potential to develop binding assays for kinases that are not recommended for use with Kinase Tracer 236, such as PIM3, CDC42BPG, MAP2K7, TXK, and SIK3. BODIPY-FL staurosporine can be a powerful tool for analyzing kinase selectivity in kinase drug discovery .
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- HY-D0142
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Fluorescent Dyes
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Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium is a fluorescent dye and pH indicator, also as a ligand of multifunctional metal-organic framework. Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium has been used to detect CO2 release. Pyrene-1,3,6,8-tetrasulfonic acid tetrasodium acts as a water-soluble fluorescent dye for fluorescence quenching assays monitoring gramicidin ion-channel activity .
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- HY-130433
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Fluorescent Dyes
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NBD Sphingosine (NBD-Sph), a fluorochrome, is a fluorescence-labeled sphingosine. NBD Sphingosine can be uesd for fluorescence assay for sphingosine kinases .
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- HY-D1190
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Fluorescent Dyes
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DC271 is a RAR agonist and synthetic retinoid that binds to the retinoid-binding site of cellular retinoic acid-binding protein II (CRBP-II). DC271 exhibits solvatochromic fluorescence properties: it produces intense blue-shifted emission in nonpolar environments and weak red-shifted emission in polar environments, and its severe fluorescence quenching in aqueous solutions can be reversed by embedding in the hydrophobic retinoid-binding protein pocket. DC271 enables direct detection of the binding between unlabeled compounds and related retinoid-binding proteins via fluorescence competition assays (Ex/Em = 355 nm/460 nm) .
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- HY-107864
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Tetraiodofluorescein
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Fluorescent Dyes
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Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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- HY-P2185
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Fluorescent Dyes
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NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .
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- HY-D2060
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Fluorescent Dyes
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ATTO 740 is a near-infrared dye (Ex/Em: 740/764 nm). ATTO 740 can be conjugated with the Cetuximab antibody (HY-P9905) .
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- HY-D1678
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Fluorescent Dyes
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5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .
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- HY-D0038
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Fluorescent Dyes
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BTBCT is mainly used as a label in time-resolved fluorescence immunoassays (TRFIA). The lower limit of detection for TSH TR-IFMA is 0.011 mIU/L in a 10 μl sample volume. The high fluorescence intensity and stability of BTBCT improves the sensitivity of the assay .
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- HY-W800831
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Fluorescent Dyes
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Tetra-sulfo-Cy7 DBCO is a bright and photostable near-IR probe. The Cy7 DBCO is water-soluble, hydrophilic dye often a reagent of choice for assay where minimal non-specific binding and exceptional brightness is required. The fluorescence of Cy7 DBCO is pH insensitive from pH 4 to pH 10 and produces minimal autofluorescence of biological specimens in this region of the spectrum. Fluorescence of this long-wavelength Cyanine dye is not visible to the human eye but is readily detected by most imaging systems.
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- HY-D2761
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Fluorescent Dyes
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DY-680-NHS ester is an amine reactive hydrophilic fluorochrome. It can be conjugated to an antibody with higher D/P ratio without causing fluorescence quenching and conjugate precipitation, and it is widely used in applications including western blotting, microscopy, flow cytometry, and cell-based assays. The spectrum has an excitation of 690nm and emission at 709nm.
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- HY-107864R
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Tetraiodofluorescein (Standard)
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Fluorescent Dyes
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Erythrosine B free acid (Standard) is the analytical standard of Erythrosine B (free acid) (HY-107864). This product is intended for research and analytical applications. Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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| Cat. No. |
Product Name |
Type |
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- HY-153232
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Biochemical Assay Reagents
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eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .
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- HY-131475
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Biochemical Assay Reagents
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Naphthol AS-BI phosphate is a substrate for acid and alkaline phosphatase and can be used in fluorescence assays. Naphthol AS-BI phosphate is a kind of biological materials or organic compounds that are widely used in life science research .
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| Cat. No. |
Product Name |
Target |
Research Area |
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- HY-P5908F
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Peptides
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Others
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FAM-DEALA-Hyp-YIPD is a fluorescent HIF-1α peptide, with the Kd of 180-560 nM. FAM-DEALA-Hyp-YIPD can be used to assess VHL binding in Fluorescence Polarization (FP) displacement assay, and evaluate the effect of VHL binding on degradation activity .
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- HY-P2185A
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MMP
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Others
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NFF-3 TFA, the peptide, is a selective MMP substrate. NFF-3 TFA selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 TFA is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 TFA with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .
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- HY-P4331
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Fluorescent Dye
Ser/Thr Protease
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Cardiovascular Disease
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Boc-Gln-Gly-Arg-AMC is a fluorogenic substrate for kallikrein (PKa), coagulation factor XIIa (FXIIa) and coagulation factor XIa (FXIa). Boc-Gln-Gly-Arg-AMC enables fluorescence-based activity assays for PKa, FXIIa and FXIa .
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- HY-P5485
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Bacterial
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Others
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Dabcyl-QALPETGEE-Edans is a synthetic peptide substrate for fluorescence resonance energy transfer (FRET) detection of Sortase A (SrtA) enzymatic activity. Dabcyl-QALPETGEE-Edans is labeled at both ends with the fluorescent group Edans (donor) and the quencher Dabcyl (acceptor), respectively. Dabcyl-QALPETGEE-Edans's central sequence contains a conserved motif specifically recognized by SrtA. Dabcyl-QALPETGEE-Edans demonstrates high sensitivity in vitro assays, enabling precise determination of the half-maximal inhibitory concentration of SrtA .
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- HY-P4725
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Fluorescent Dye
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Others
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Abz-Thr-Ile-Nle-p-nitro-Phe-Gln-Arg-NH2 is a fluorogenic substrate, that can be used for the fluorescence screening assay .
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- HY-P2185
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MMP
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Others
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NFF-3, the peptide, is a selective MMP substrate. NFF-3 selectively binds to MMP-3 and MMP-10 to be hydrolyzed. NFF-3 is also cleaved by trypsin, hepatocyte growth factor activator, and factor Xa. Label NFF-3 with a CyDye pair, Cy3/Cy5Q, can produce fluorescence in cell assays to detect cell activity .
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- HY-P10344
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Fluorescent Dye
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Infection
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MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro .
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- HY-P5415
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HIV
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Others
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DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is a biological active peptide. (DABCYL-GABA-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-EDANS is also called HIV protease substrate I in some literature. It is widely used for the continuous assay for HIV protease activity. The 11-Kd protease (PR) encoded by the human immunodeficiency virus 1 (HIV-1) is essential for the correct processing of viral polyproteins and the maturation of infectious virus, and is therefore a target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of recombinant HIV-1 PR with the fluorogenic substrate resulted in specific cleavage at the Tyr-Pro bond and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. The fluorescence quantum yields of the HIV-1 PR substrate in the FRET assay increased by 40.0- and 34.4-fold, respectively, per mole of substrate cleaved. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this substrate offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs. Abs/Em = 340nm/490nm.)
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- HY-P11481
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Fluorescent Dye
E1/E2/E3 Enzyme
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Others
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FITC-Acp-RpYTPEP-NH2 is a potent fluorescent tracer. FITC-Acp-RpYTPEP-NH2 shows a binding affinity (Kd = 0.07 μM) for the Cbl-b SH2 domain. Fluorescence polarization assay based on FITC-Acp-RpYTPEP-NH2 can be for evaluating Cbl-b SH2 domain inhibitors .
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- HY-K6016
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2 Publications Verification
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MCE Organoid Vitality Assay Kit is a fluorescence-based assay kit specifically designed for measuring cell viability in organoids.
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- HY-K0322
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4 Publications Verification
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MCE Cell Ferrous Iron (Fe2+) Assay Kit (Fluorometric) utilizes fluorescence detection technology to analyze the levels of ferrous ions (Fe2+) in cells.
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| Cat. No. |
Product Name |
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Classification |
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- HY-153232
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Liposome
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eGFP circRNA-LNP is a lipid nanoparticle (LNP) containing eGFP circRNA, suitable for assays of RNA delivery, translation efficiency, cell viability, etc. eGFP circRNA carries Enhanced Green Fluorescent Protein (Enhanced Green Fluorescent Protein) eGFP, which will express green fluorescent protein after entering the cell. eGFP is commonly used as a reporter gene detectable by fluorescence microscopy or flow cytometry .
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