6-TAMRA  (Synonyms: 6-Carboxytetramethylrhodamine)

6-TAMRA (6-Carboxytetramethylrhodamine) is a fluorescent dye. 6-TAMRA is often used in combination with 6-FAM (HY-66021) as an acceptor or fluorescence quencher for fluorescence resonance energy transfer (FRET) in biochemical studies such as potassium ion detection and ribonuclease activity assays^[1][2].

6-TAMRA, in the form of succinimidyl ester, undergoes conjugation with 3'-amino-modified oligonucleotides via incubation at 37℃ for 12 h in a pH 8.5 sodium bicarbonate buffer, and is further used as a 3'-end labeling dye for fluorescence quenching^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

430.45

C₂₅H₂₂N₂O₅

91809-67-5

Solid

Brown to black

580

565

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC(C([O-])=O)=CC=C4C(O)=O)=C2C=C1)C

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Ueyama H, et al. A novel potassium sensing in aqueous media with a synthetic oligonucleotide derivative. Fluorescence resonance energy transfer associated with Guanine quartet-potassium ion complex formation. J Am Chem Soc. 2002;124(48):14286-14287.  [Content Brief]

  [2]. Kelemen BR, et al. Hypersensitive substrate for ribonucleases. Nucleic Acids Res. 1999;27(18):3696-3701.  [Content Brief]