Geldanamycin-FITC
Based on 1 publication(s) in Google Scholar
Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90 (Ex/Em = 488/515 nm).
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- Pureza : 92.56%
- No. CAS: 2969156-01-0
- Fòrmula: C55H63N5O13S
- Peso molecular:1034.18
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Almacenamiento:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Geldanamycin-FITC
More
Actividad biológica
Descripciòn
IC50 & Target
[1]|
HSP90 |
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[2].
1. Solution preparation
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-10 mM (optimized according to the experiment).
1.2 Preparation of working solution
Dilute to 1 nM-10 μM with buffer C (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Competition binding assays
2.1 HsHsp90 and PfHsp90 are added at final concentrations of 32 nM and 60 nM, respectively.
2.2 Assay mixtures contained 1 nM Geldanamycin-FITC in buffer C in 384-well microplates.
2.3 The final assay volume is 50 μL in all wells.
2.4 Plates are incubated overnight at 4 °C, followed by centrifugation.
2.5 The fluorescence polarization (FP) signal in each well is measured by use of a 480-nm excitation filter and a 535-nm emission filter on an EnVision system.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
Emission (Em)
515
Excitation (Ex)
488
Chemical Information
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No. CAS 2969156-01-0
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Appearance Solid
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Peso molecular 1034.18
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Fòrmula C55H63N5O13S
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Color Light brown to brown
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SMILES
O=C(C(NCCCCCCNC(NC1=CC=C(C(C(C=CC(O)=C2)=C2O3)=C4C3=CC(C=C4)=O)C(C(O)=O)=C1)=S)=C5C[C@@H](C)C[C@H](OC)[C@H](O)[C@@H](C)/C=C6C)C=C(NC(/C(C)=C/C=C\[C@H](OC)[C@H]\6OC(N)=O)=O)C5=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Bioorg Chem
Novel Hsp90 inhibitors as leads for the development of radiotheragnostics for Meitner-Auger electron therapy. [Abstract]2026 Mar:170:109522. PMID: 41576901
Solvente y solubilidad
In Vitro:
DMSO : 25 mg/mL (24.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Kim J, et al. Development of a fluorescence polarization assay for the molecular chaperone Hsp90. J Biomol Screen. 2004;9(5):375‐381. [Content Brief]
[2]. Posfai D, et al. Identification of Hsp90 Inhibitors with Anti-Plasmodium Activity. Antimicrob Agents Chemother. 2018;62(4):e01799-17. Published 2018 Mar 27. [Content Brief]
[3]. Imai T, et al. Heat shock protein 90 (HSP90) contributes to cytosolic translocation of extracellular antigen for cross-presentation by dendritic cells. Proc Natl Acad Sci U S A. 2011;108(39):16363‐16368. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 0.9669 mL | 4.8347 mL | 9.6695 mL | 24.1737 mL |
| 5 mM | 0.1934 mL | 0.9669 mL | 1.9339 mL | 4.8347 mL | |
| 10 mM | 0.0967 mL | 0.4835 mL | 0.9669 mL | 2.4174 mL | |
| 15 mM | 0.0645 mL | 0.3223 mL | 0.6446 mL | 1.6116 mL | |
| 20 mM | 0.0483 mL | 0.2417 mL | 0.4835 mL | 1.2087 mL |
Geldanamycin-FITC Related Classifications
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