Geldanamycin-FITC 

Geldanamycin-FITC, a Geldanamycin fluorescent probe, can be used in a fluorescence polarization assay for HSP90 inhibitors. Geldanamycin-FITC also can be used for detection of cell surface HSP90^[1][2][3].

The HSP90 inhibitor Geldanamycin, when conjugated with FITC (GA–FITC) is cell-membrane impermeable, binding only cell surface HSP90 and inhibiting its functions but having no effect on cytosolic HSP90^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1034.18

C₅₅H₆₃N₅O₁₃S

2969156-01-0

Solid

Light brown to brown

515

488

O=C(C(NCCCCCCNC(NC1=CC=C(C(C(C=CC(O)=C2)=C2O3)=C4C3=CC(C=C4)=O)C(C(O)=O)=C1)=S)=C5C[C@@H](C)C[C@H](OC)[C@H](O)[C@@H](C)/C=C6C)C=C(NC(/C(C)=C/C=C\[C@H](OC)[C@H]\6OC(N)=O)=O)C5=O

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Kim J, et al. Development of a fluorescence polarization assay for the molecular chaperone Hsp90. J Biomol Screen. 2004;9(5):375‐381.  [Content Brief]

  [2]. Posfai D, et al. Identification of Hsp90 Inhibitors with Anti-Plasmodium Activity. Antimicrob Agents Chemother. 2018;62(4):e01799-17. Published 2018 Mar 27.  [Content Brief]

  [3]. Imai T, et al. Heat shock protein 90 (HSP90) contributes to cytosolic translocation of extracellular antigen for cross-presentation by dendritic cells. Proc Natl Acad Sci U S A. 2011;108(39):16363‐16368.  [Content Brief]