c-Myc agarose gel

Glutamine is an important metabolic fuel that helps rapidly proliferating cells meet the increased demand for ATP, biosynthetic precursors, and reducing agents. Glutamine Metabolism pathway involves the initial deamination of glutamine by glutaminase(GLS), yielding glutamate and ammonia. Glutamate is converted to the TCA cycle intermediate α-ketoglutarate (α-KG) by either glutamate dehydrogenase (GDH) or by the alanine or aspartate transaminases (TAs), to produce both ATP and anabolic carbons for the synthesis of amino acids, nucleotides and lipids. During periods of hypoxia or mitochondrial dysfunction, α-KG can be converted to citrate in a reductive carboxylation reaction catalyzed by IDH2. The newly formed citrate exits the mitochondria where it is used to synthesize fatty acids and amino acids and produce the reducing agent, NADPH.

Cancer cells display an altered metabolic circuitry that is directly regulated by oncogenic mutations and loss of tumor suppressors. Mounting evidence indicates that altered glutamine metabolism in cancer cells has critical roles in supporting macromolecule biosynthesis, regulating signaling pathways, and maintaining redox homeostasis, all of which contribute to cancer cell proliferation and survival. Thus, intervention in glutamine metabolic processes could provide novel approaches to improve cancer treatment.

MCE owns a unique collection of 1,730 compounds targeting the mainly proteins and enzymes involved in glutamine metabolism pathway. Glutamine Metabolism compound library is a useful tool for intervention in glutamine metabolic processes.

Techniques for reprogramming somatic cells create new opportunities for drug screening, disease modeling, artificial organ development, and cell therapy. The development of reprogramming techniques has grown exponentially since Yamanaka reprogrammed somatic cells to become induced pluripotent stem cells (iPSCs) using four transcription factors, OCT4, SOX2, KLF4, and c-MYC in 2006. Despite the development of efficient reprogramming methods, most methods are inappropriate for clinical applications because they carry the risk of integrating exogenous genetic factors or use oncogenes. Alternative approaches, such as those based on miRNA, non-viral genes, non-integrative vectors, and small molecules, have been studied as possible solutions to the problems. Among these alternatives, small molecules are attractive options for clinical applications. Reprogramming using small molecules is inexpensive and easy to control in a concentration- and time-dependent manner. It offers a high level of cell permeability, ease of synthesis and standardization, and it is appropriate for mass-producing cells.

MCE Reprogramming Compound Library contains a unique collection of 3,129 compounds that act on reprogramming signaling pathways. These compounds are potential stimulators for reprogramming. This library is a useful tool for researching reprogramming and regenerative medicine.

Coagulation, also known as clotting, is the process in which blood changes from a liquid to a solid gel to form a blood clot. Thrombin, which is accurately and evenly generated in the injured part of blood vessels, is a key effector enzyme of the blood coagulation system and participates in many important biological processes, such as platelet activation, fibrinogen conversion to fibrin network, coagulation feedback amplification, etc. At the same time, to avoid the accidental formation of thrombus in the body, there is also an anticoagulant mechanism that inhibits blood coagulation.

Normal coagulation mechanism represents a balance between the pro-coagulant pathway in the injured site and anti-coagulant pathway beyond it. The blood coagulation system may be out of balance during the perioperative period or critical illness, which may lead to thrombosis or excessive bleeding. Therefore, the physiological study of coagulation balance is an important basis for clinical diagnosis and treatment of the abnormal coagulation process.

MCE supplies a unique collection of 1,460 compounds targeting key proteins in coagulation and anti-coagulation system. MCE Coagulation and Anti-coagulation Compound Library is a useful tool for study the mechanism of coagulation and anticoagulation.

MCE Anti-c-Myc Affinity Gel can be used for the detection and purification of c-Myc fusion expressed proteins, and immunoprecipitation (IP) experiments to detect the expression of recombinant proteins in target cells.

MCE Red Nucleic Acid Gel Stain (10,000×) is a nucleic acid stain that can be used as a safer alternative to the traditional ethidium bromide (EB) stain for detecting nucleic acids in agarose gels or polyacrylamide gels.

MCE SYBR Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels.

MCE Agarose With TAE Powder (1%) mainly consists of proportionally mixed agarose and TAE Powder, and can be directly made into 1% agarose gel. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Agarose can be made into 0.5-2.5% agarose gel according to different needs and can resolve DNA and RNA fragments from 50-15,000 bp.

MCE TBE Powder (1 L of 1×) consists of Tris, Boric Acid and EDTA, and is widely used in agarose gel electrophoresis of nucleic acid. The 10 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE TAE Powder (1 L of 1×) consists of Tris-acetate and EDTA-2Na, and is widely used in agarose gel electrophoresis of nucleic acid. The 20 pouches are defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Anti-c-Myc Magnetic Beads are used for immunoprecipitation (IP) of specific c-Myc-tagged proteins expressed in bacterial and mammalian cells and in vitro expression systems. The 1 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE Glutathione Agarose, a 4% highly cross-linked agarose reagent, enables high-yield, high-purity purification of GST-tagged proteins.

MCE Streptavidin Agarose 6FF, a 6% highly cross-linked agarose reagent coupled with recombinant streptavidin, is an affinity chromatography medium for separation and purification of biotinylated peptides, antibodies, lectins, etc. The total binding capacity of Streptavidin Agarose 6FF is more than 200 nmol of D-Biotin/mL settled resin.

MCE Ni-NTA His-Tag Purification Agarose, a 6% highly cross-linked agarose reagent, enables high-yield, high-purity purification of his-tagged proteins.

MCE Protein A Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein A, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG, IgE, IgM.

MCE Butyl Agarose 4FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Octyl Agarose 6FF is a hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Phenyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules.

MCE Protein L Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein L, effectively purifies mammalian monoclonal and polyclonal antibodies which have kappa light chain.

MCE High-Affinity Iodoacetyl Agarose, a 4% highly cross-linked agarose reagent coupled with a derivative of iodoacetic acid, is ideal for conjugating sulfhydryl-containing peptide or protein for subsequent affinity purification.

MCE Butyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling butyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE Octyl Agarose HP is a high-resolution hydrophobic chromatography medium formed by covalently coupling octyl ligands to agarose. It is suitable for both laboratory use and large-scale industrial purification.

MCE CM Agarose 6FF is a weak cation exchanger formed by covalently coupling carboxymethyl (CM) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE SP Agarose 6FF is a strong cation exchanger formed by covalently coupling sulfopropyl (SP) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE DEAE Agarose 6FF is a weak cation exchanger formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose microspheres. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE Q Agarose 6FF is a strong cation exchanger formed by covalently coupling quarternary ammonium (Q) groups to agarose. It is suitable for both laboratory research and large-scale industrial purification processes.

MCE Protein G Agarose, a 4% highly cross-linked agarose reagent coupled with recombinant Protein G, effectively purifies mammalian monoclonal and polyclonal antibodies, such as human IgG3 and rat IgG2a.

MCE One Step Colored Rapid Gel Preparation Kit (12.5%) uses pre-mixed formulations of the upper gel and lower gel. Simply mix the reagents in pairs, add modified coagulation agent, and the gel will form quickly and easily.

MCE Phenyl Agarose (Low Sub) 6FF is a low-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

MCE Phenyl Agarose (High Sub) 6FF is a high-substitution hydrophobic chromatography medium formed by covalently coupling phenyl ligands to agarose. It is suitable for laboratory-scale and industrial-scale purification of biomolecules with relatively weak hydrophobicity.

MCE MBP Agarose (Dextrin) 6FF is prepared by covalently coupling dextrin to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. It can achieve one-step purification of MBP fusion protein.

MCE SP Agarose HP is a high-resolution strong cation exchanger formed by covalently coupling sulfopropyl (SP) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

MCE DEAE Agarose HP is a high-resolution weak anion exchanger formed by covalently coupling diethylaminoethyl (DEAE) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

MCE Protein A/G Agarose is an affinity chromatography medium for separation and purification of immunoglobulins.

MCE Ni-IDA Agarose can be used for the detection and purification of His-tag proteins.

MCE Q Agarose HP is a high-resolution strong anion exchanger formed by covalently coupling quaternary ammonium (Q) groups to agarose. This medium offers high binding capacity, high specificity, and excellent ligand stability, making it suitable for both laboratory-scale and large-scale industrial purification applications.

MCE IMAC Agarose (NTA) 6FF is prepared by covalently coupling tetradentate nitrilotriacetic acid (NTA) to an agarose matrix. It features high binding capacity, excellent specificity, and superior ligand stability. The resin allows flexible chelation with metal ions such as Zn²⁺, Ni²⁺, or Cu²⁺, and is suitable for the purification of His-tagged recombinant proteins expressed in bacterial, mammalian, insect, and baculovirus systems.

MCE Anti-HA Magnetic Agarose Beads can be used for the detection and purification of HA fusion-expressed proteins and IP assays.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of GFP, EGFP, and their fusion-expressed proteins and IP assays.

MCE Ni-NTA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Anti-GFP Magnetic Agarose Beads can be used for the detection and purification of Flag (DYKDDDDK) fusion-expressed proteins and IP assays.

MCE Ni-IDA Magnetic Agarose Beads can be used for the detection and purification of His-tag proteins from various expression sources.

MCE Protein A Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Protein G Plus Magnetic Agarose Beads can be used for the detection and purification of IgG from serum, ascites fluid, cell culture supernatant and other antibody samples.

MCE Protein A Agarose (Alkali-Tolerant) 4FF can efficiently binds IgG from serum, ascites, culture supernatants, and other antibody-containing samples, enabling high-performance antibody purification.

MCE Glutathione Magnetic Agarose Beads have high protein-binding capacity and stability, making it ideal for high performance purification of GST-tagged fusion proteins expressed in E. coli, yeast, insect and mammalian expression systems.

MCE Blue Agarose 6FF can be used for the purification of biological macromolecules such as albumin, interferons, nucleotide-dependent enzymes (e.g., kinases, dehydrogenases), α2-macroglobulin, and coagulation factors.

MCE Anti-RFP Affinity Gel can be used for the detection and purification of native RFP, RFP mutants, and IP assays.

MCE Anti-YFP Affinity Gel can be used for the detection and purification of proteins tagged with GFP and EGFP and IP assays.

MCE Chelating Agarose (IDA) 6FF allows flexible chelation with metal ions such as Zn²⁺, Ni²⁺, or Cu²⁺, and is suitable for the purification of His-tagged recombinant proteins expressed in bacterial, mammalian, insect, and baculovirus systems.

MCE Anti-GFP Affinity Gel can be used for the detection and purification of GFP, EGFP, their fusion-expressed proteins and IP assays.

MCE Coomassie Blue Fast Staining Solution (No Heating, 10×) uses Coomassie Brilliant Blue G250 as the dye. It can be used for rapid, sensitive staining of protein gels in SDS-PAGE or Native-PAGE without contamination, or for detecting residual proteins on PAGE gels after Western Blot transfer. The 25 mL is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

MCE GelHP Protein Gel offers a safe, convenient, and high‑performance solution for protein separation, suitable for both PAGE and Western blot analysis.