SHP2 protein degrader-3
SHP2 protein degrader-3 is a SHP2 AUTAC degrader. SHP2 protein degrader-3 shows dose-dependent SHP2 degradation ability (DC50 = 3.22 μM) and anti-tumor activity (IC50 = 5.59 μM) in HeLa cells. SHP2 protein degrader-3 induces degradation through the LC3-mediated autophagy pathway, which can be inhibited by lysosome inhibitors. SHP2 protein degrader-3 induces apoptosis in various cancer cells (HeLa cells, HepG2 cells, LoVo cells, Huh-7 cells) (SHP2 Ligand : (HY-100388); LC3 Ligand: (HY-10542); Linker : (HY-128834)).
For research use only. We do not sell to patients.
- Formula: C46H51Br2Cl2IN10O7
- Molecular Weight:1213.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
SHP2 protein degrader-3 (Compound SA-8) (24-72 h) exhibits anti-proliferative activity against HeLa cells, with IC50s of 5.59 μM (24 h), 4.25 μM (48 h) and 4.81 μM (72 h)[1].
SHP2 protein degrader-3 (0-10 μM, 24-96 h) degrades SHP2 in a time- and dose-dependent manner in HeLa cells, with a DC50 of 3.22 μM and Dmax of 80.47%[1].
SHP2 protein degrader-3 (10 μM, 72 h) induces SHP2 degradation via autophagy-lysosome pathway[1].
SHP2 protein degrader-3 (1.1-30 μM, 72 h) induces apoptosis at different concentrations in cancer cells (HeLa cells, HepG2 cells, LoVo cells, Huh-7 cells)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HeLa cells
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Concentration:10 μM
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Incubation Time:72 h
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Result:Blocked SHP2 degradation when Pre-treatment with GW5074 (HY-10542) (1 μM) or SHP099 (HY-100388) (1 μM) for 4 h.
Blocked SHP2 degradation when Pre-treatment with Chloroquine (HY-17589A) (1 μM) or 3-Methyladenine (3-MA) (HY-19312) (0.125 nM/1 μM) for 4 h.
Blocked SHP2 degradation when Pre-treatment with MG132 (HY-13259) (50 nM) for 4 h.
Enhanced SHP2 degradation when Pre-treatment with Rapamycin (HY-10219) (6 μM) for 4 h.
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Cell Line:HeLa cells
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Concentration:0.12, 0.37, 1.1, 3.3, 10 μM
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Incubation Time:48 h
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Result:Increased the LC3 levels in HeLa cells.
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Cell Line:HeLa cells, HepG2 cells, LoVo cells, Huh-7 cells
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Concentration:1.1, 3.3, 10, 20, 30 μM
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Incubation Time:72 h
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Result:Induced apoptosis at different concentrations in HeLa cells, HepG2 cells, LoVo cells, Huh-7 cells.
Chemical Information
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Molecular Weight 1213.58
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Formula C46H51Br2Cl2IN10O7
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SMILES
CC1(CCN(CC1)C2=CN=C(C(N)=N2)C3=CC=CC(Cl)=C3Cl)NCC4=CN(CCOCCOCCOCCOCCNC(COC5=C(C=C(C=C5Br)/C=C6C(NC7=C\6C=C(C=C7)I)=O)Br)=O)N=N4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
SHP2 protein degrader-3 Related Classifications
HY-175204 Related Classifications
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