SS-208
Based on 1 Customer Validation
SS-208 is a selective HDAC6 inhibitor, with an IC50 of 12 nM. SS-208 possesses anti-tumor activity in melanoma.
For research use only. We do not sell to patients.
- Purity: 99.00%
- CAS No.: 2245942-72-5
- Formula: C13H11Cl2N3O4
- Molecular Weight:344.15
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Storage:Powder -20°C, 3 years
* The compound is unstable in solutions, freshly prepared is recommended.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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HDAC6 12 nM (IC50) |
HDAC8 1.23 μM (IC50) |
HDAC1 1.39 μM (IC50) |
HDAC11 5.12 μM (IC50) |
HDAC5 6.91 μM (IC50) |
HDAC7 8.34 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | IC50 |
>30 μM
Compound: 7b; SS-208
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Inhibition of human ERG expressed in HEK293 cells by manual-patch clamp assay
Inhibition of human ERG expressed in HEK293 cells by manual-patch clamp assay
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[PMID: 31414801] |
| HEK293 | IC50 |
0.504 μM
Compound: 7b; SS-208
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Inhibition of HDAC6 CD2 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase incubated for 2 hrs followed by NanoBRET NanoGlo Substrate addition by NanoBRET assay
Inhibition of HDAC6 CD2 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase incubated for 2 hrs followed by NanoBRET NanoGlo Substrate addition by NanoBRET assay
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[PMID: 31414801] |
| HEK293 | IC50 |
6.673 μM
Compound: 7b; SS-208
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Inhibition of HDAC1 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase incubated for 2 hrs followed by NanoBRET NanoGlo Substrate addition by NanoBRET assay
Inhibition of HDAC1 (unknown origin) expressed in HEK293 cells cotransfected with nano-luciferase incubated for 2 hrs followed by NanoBRET NanoGlo Substrate addition by NanoBRET assay
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[PMID: 31414801] |
| Sf9 | IC50 |
0.075 μM
Compound: 7b; SS-208
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Inhibition of recombinant N-terminal GST-tagged human HDAC6 (1 to 1215 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by s
Inhibition of recombinant N-terminal GST-tagged human HDAC6 (1 to 1215 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mins followed by s
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[PMID: 31414801] |
| Sf9 | IC50 |
31.5 μM
Compound: 7b; SS-208
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Inhibition of recombinant full length C-terminal His/FLAG tagged human HDAC1 (1 to 482 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mi
Inhibition of recombinant full length C-terminal His/FLAG tagged human HDAC1 (1 to 482 residues) expressed in baculovirus infected sf9 insect cells using p53 (379 to 382 residues) derived fluorogenic peptide RHKKAc as substrate preincubated for 5 to 10 mi
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[PMID: 31414801] |
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice injected immunogenic murine SM1 melanoma cells subcutaneously[1].
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Dosage:25 mg/kg.
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Administration:IP on day 4, 7, 12, 15 and 18.
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Result:Significantly reduced the tumor growth.
Chemical Information
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CAS No. 2245942-72-5
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Appearance Solid
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Molecular Weight 344.15
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Formula C13H11Cl2N3O4
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Color White to off-white
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SMILES
O=C(C1=NOC(CCNC(C2=CC=C(Cl)C(Cl)=C2)=O)=C1)NO
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Synonyms
AVS100
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years * The compound is unstable in solutions, freshly prepared is recommended.
Solvent & Solubility
DMSO : 125 mg/mL (363.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.04 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * The compound is unstable in solutions, freshly prepared is recommended.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (604 KB)
- English - EN (604 KB)
- Français - FR (604 KB)
- Deutsch - DE (604 KB)
- Norwegian - NO (604 KB)
- Español - ES (604 KB)
- Swedish - SV (604 KB)
- Italian - IT (604 KB)
- Portuguese - PT (604 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9057 mL | 14.5285 mL | 29.0571 mL | 72.6427 mL |
| 5 mM | 0.5811 mL | 2.9057 mL | 5.8114 mL | 14.5285 mL | |
| 10 mM | 0.2906 mL | 1.4529 mL | 2.9057 mL | 7.2643 mL | |
| 15 mM | 0.1937 mL | 0.9686 mL | 1.9371 mL | 4.8428 mL | |
| 20 mM | 0.1453 mL | 0.7264 mL | 1.4529 mL | 3.6321 mL | |
| 25 mM | 0.1162 mL | 0.5811 mL | 1.1623 mL | 2.9057 mL | |
| 30 mM | 0.0969 mL | 0.4843 mL | 0.9686 mL | 2.4214 mL | |
| 40 mM | 0.0726 mL | 0.3632 mL | 0.7264 mL | 1.8161 mL | |
| 50 mM | 0.0581 mL | 0.2906 mL | 0.5811 mL | 1.4529 mL | |
| 60 mM | 0.0484 mL | 0.2421 mL | 0.4843 mL | 1.2107 mL | |
| 80 mM | 0.0363 mL | 0.1816 mL | 0.3632 mL | 0.9080 mL | |
| 100 mM | 0.0291 mL | 0.1453 mL | 0.2906 mL | 0.7264 mL |
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