2. Metabolic Enzyme/Protease
  3. Ser/Thr Protease
  4. Suc-Phe-Ala-Ala-Phe-pNA

Suc-Phe-Ala-Ala-Phe-pNA  (Synonyms: Suc-FAAF-pNA)

Cat. No.: HY-P4582 Purity: 99.74%
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Suc-Phe-Ala-Ala-Phe-pNA (Suc-FAAF-pNA) is a chromogenic polypeptide substrate that can be used for the quantitative activity assay of intracellular subtilisin (ISP) and neutral metalloprotease (NPS) derived from Saccharomonospora canescens. Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the catalytic triad of mature proteases to release the chromophore pNA. Suc-Phe-Ala-Ala-Phe-pNA is applicable to enzymological studies.

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Suc-Phe-Ala-Ala-Phe-pNA

Suc-Phe-Ala-Ala-Phe-pNA Chemical Structure

CAS No. : 110906-89-3

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Suc-Phe-Ala-Ala-Phe-pNA Related Antibodies

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Description

Suc-Phe-Ala-Ala-Phe-pNA (Suc-FAAF-pNA) is a chromogenic polypeptide substrate that can be used for the quantitative activity assay of intracellular subtilisin (ISP) and neutral metalloprotease (NPS) derived from Saccharomonospora canescens. Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the catalytic triad of mature proteases to release the chromophore pNA. Suc-Phe-Ala-Ala-Phe-pNA is applicable to enzymological studies[1][2].

In Vitro

Suc-Phe-Ala-Ala-Phe-pNA (50 μM; 5 h) is a substrate for processed Bacillus clausii NΔ18-ISP, which exhibits proteolytic activity toward it; Suc-Phe-Ala-Ala-Phe-pNA (4-75 μM) also enables quantification of N-terminal extension peptide inhibition of NΔ18-ISP with a Ki of 1 μM[1].
Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the purified neutral metalloproteinase NPS from Saccharomonospora canescens sp. novus, strain 5 with a Km of 14.5 μM, kcat of 1.20 s-1[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Suc-Phe-Ala-Ala-Phe-pNA Related Antibodies
Molecular Weight

674.70

Formula

C34H38N6O9
CAS No.
Appearance

Solid

Color

White to off-white

Sequence

Suc-Phe-Ala-Ala-Phe-pNA
Sequence Shortening

Suc-FAAF-pNA
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (148.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4821 mL 7.4107 mL 14.8214 mL
5 mM 0.2964 mL 1.4821 mL 2.9643 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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This equation is commonly abbreviated as: C1V1 = C2V2

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Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4821 mL 7.4107 mL 14.8214 mL 37.0535 mL
5 mM 0.2964 mL 1.4821 mL 2.9643 mL 7.4107 mL
10 mM 0.1482 mL 0.7411 mL 1.4821 mL 3.7054 mL
15 mM 0.0988 mL 0.4940 mL 0.9881 mL 2.4702 mL
20 mM 0.0741 mL 0.3705 mL 0.7411 mL 1.8527 mL
25 mM 0.0593 mL 0.2964 mL 0.5929 mL 1.4821 mL
30 mM 0.0494 mL 0.2470 mL 0.4940 mL 1.2351 mL
40 mM 0.0371 mL 0.1853 mL 0.3705 mL 0.9263 mL
50 mM 0.0296 mL 0.1482 mL 0.2964 mL 0.7411 mL
60 mM 0.0247 mL 0.1235 mL 0.2470 mL 0.6176 mL
80 mM 0.0185 mL 0.0926 mL 0.1853 mL 0.4632 mL
100 mM 0.0148 mL 0.0741 mL 0.1482 mL 0.3705 mL
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Suc-Phe-Ala-Ala-Phe-pNA Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Suc-Phe-Ala-Ala-Phe-pNA110906-89-3Suc-FAAF-pNASer/Thr ProteaseSerine proteasesSerine endopeptidasesThreonine proteasesproteolytic activitySaccharomonospora canescenskinetic analysisN-terminal extension peptidearyl amidaseintracellular subtilisin proteaseproISPneutral metalloproteinase NPSBacillus clausiiNΔ18-ISPInhibitorinhibitorinhibit

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