Suc-Phe-Ala-Ala-Phe-pNA
Based on 1 Customer Validation
Suc-Phe-Ala-Ala-Phe-pNA (Suc-FAAF-pNA) is a chromogenic polypeptide substrate that can be used for the quantitative activity assay of intracellular subtilisin (ISP) and neutral metalloprotease (NPS) derived from Saccharomonospora canescens. Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the catalytic triad of mature proteases to release the chromophore pNA. Suc-Phe-Ala-Ala-Phe-pNA is applicable to enzymological studies.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 110906-89-3
- Formula: C34H38N6O9
- Molecular Weight:674.70
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Suc-Phe-Ala-Ala-Phe-pNA (50 μM; 5 h) is a substrate for processed Bacillus clausii NΔ18-ISP, which exhibits proteolytic activity toward it; Suc-Phe-Ala-Ala-Phe-pNA (4-75 μM) also enables quantification of N-terminal extension peptide inhibition of NΔ18-ISP with a Ki of 1 μM[1].
Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the purified neutral metalloproteinase NPS from Saccharomonospora canescens sp. novus, strain 5 with a Km of 14.5 μM, kcat of 1.20 s-1[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 110906-89-3
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Appearance Solid
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Molecular Weight 674.70
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Formula C34H38N6O9
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Color White to off-white
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Synonyms
Suc-FAAF-pNA
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Sequence
Suc-Phe-Ala-Ala-Phe-pNA
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Sequence Shortening
Suc-FAAF-pNA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 100 mg/mL (148.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Handling Instructions (2659 KB)
References
[1]. Gamble M, et al. Regulation of an intracellular subtilisin protease activity by a short propeptide sequence through an original combined dual mechanism. Proc Natl Acad Sci U S A. 2011;108(9):3536-3541. [Content Brief]
[2]. Dolashka P, et al. A novel thermostable neutral proteinase from Saccharomonospora canescens. Biochim Biophys Acta. 1998;1382(2):207-216. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4821 mL | 7.4107 mL | 14.8214 mL | 37.0535 mL |
| 5 mM | 0.2964 mL | 1.4821 mL | 2.9643 mL | 7.4107 mL | |
| 10 mM | 0.1482 mL | 0.7411 mL | 1.4821 mL | 3.7054 mL | |
| 15 mM | 0.0988 mL | 0.4940 mL | 0.9881 mL | 2.4702 mL | |
| 20 mM | 0.0741 mL | 0.3705 mL | 0.7411 mL | 1.8527 mL | |
| 25 mM | 0.0593 mL | 0.2964 mL | 0.5929 mL | 1.4821 mL | |
| 30 mM | 0.0494 mL | 0.2470 mL | 0.4940 mL | 1.2351 mL | |
| 40 mM | 0.0371 mL | 0.1853 mL | 0.3705 mL | 0.9263 mL | |
| 50 mM | 0.0296 mL | 0.1482 mL | 0.2964 mL | 0.7411 mL | |
| 60 mM | 0.0247 mL | 0.1235 mL | 0.2470 mL | 0.6176 mL | |
| 80 mM | 0.0185 mL | 0.0926 mL | 0.1853 mL | 0.4632 mL | |
| 100 mM | 0.0148 mL | 0.0741 mL | 0.1482 mL | 0.3705 mL |
Suc-Phe-Ala-Ala-Phe-pNA Related Classifications
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