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Cat. No.: HY-152187

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Topoisomerase IIα-IN-5

Topoisomerase IIα-IN-5 is a topoisomerase II (topo II) α catalytic inhibitor. Topoisomerase IIα-IN-5 intercalates into DNA and binds to the DNA minor groove. Topoisomerase IIα-IN-5 exhibits better efficacy and less genotoxicity than Etoposide (HY-13629).

For research use only. We do not sell to patients.

CAS No.: 3037545-23-3
Formula: C₂₅H₃₀N₂O₅
Molecular Weight:438.52

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

Topoisomerase IIα-IN-5 Chemical Structure

CAS No.: 3037545-23-3

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

topoisomerase II alpha

All Topoisomerase Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
DU-145	IC₅₀	0.13 μM Compound: 6	Antiproliferative activity against human DU-145 cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay Antiproliferative activity against human DU-145 cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay	[PMID: 36493620]
HCT-15	IC₅₀	9.25 μM Compound: 6	Antiproliferative activity against human HCT-15 cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay Antiproliferative activity against human HCT-15 cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay	[PMID: 36493620]
T47D	IC₅₀	0.53 μM Compound: 6	Antiproliferative activity against human T47D cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay Antiproliferative activity against human T47D cells assessed as inhibition of cell proliferation incubated for 72 hrs by WST assay	[PMID: 36493620]

In Vitro

Topoisomerase IIα-IN-5 (compound 6) (5 μM, 10 μM, and 20 μM; 10 d) inhibits colony formation of DU145 cells^[1].
Topoisomerase IIα-IN-5 (1 μM and 10 μM; 72 h) shows antiproliferative against DU145 cells^[1].
Topoisomerase IIα-IN-5 has cytotoxicity against DU145, HCT15, and T47D cells with IC₅₀s of 0.13 μM, 9.25 μM, and 0.53 μM, respectively^[1].
Topoisomerase IIα-IN-5 (200 μM) interacts with a DNA double helix^[1].
Topoisomerase IIα-IN-5 (20 μM; 24 h) induces G2 cell cycle arrest and apoptosis in CRPC cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

CRPC cells
Concentration:

0, 5, 10, and 20 μM
Incubation Time:

0, 8, 16, and 24 hours
Result:

Increased the expression of both phosphorylated cdc25c and phosphorylated cdc2.

Apoptosis Analysis^[1]

Cell Line:

CRPC cells
Concentration:

0, 5, 10, and 20 μM
Incubation Time:

0, 8, 16, and 24 hours
Result:

Induced cell cycle arrest at G2 phase.
Induced cell apoptosis.

Chemical Information

CAS No. 3037545-23-3
Molecular Weight 438.52
Formula C₂₅H₃₀N₂O₅
SMILES

O=C1C=C(OC2=CC(OCC(CNCCN3CCCCC3)O)=CC(O)=C12)C4=CC=CC=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Jeon KH, et al. Synthesis and evaluation of 7-(3-aminopropyloxy)-substituted flavone analogue as a topoisomerase IIα catalytic inhibitor and its sensitizing effect to enzalutamide in castration-resistant prostate cancer cells. Eur J Med Chem. 2023 Jan 15;246:114999. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

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100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Topoisomerase IIα-IN-5

All Topoisomerase Isoforms