Tubulin/CDC5L-IN-1
Based on 1 Customer Validation
Tubulin/CDC5L-IN-1 is a Tubulin/CDC5L dual inhibitor. Tubulin/CDC5L-IN-1 targets to CDCL5 with a KD of 103.7 μM. Tubulin/CDC5L-IN-1 can inhibit multiple cancer cell proliferation and induce G2/M phase arrest. Tubulin/CDC5L-IN-1 induce cell apoptosis and ROS production. Tubulin/CDC5L-IN-1 exhibits antiangiogenic effects. Tubulin/CDC5L-IN-1 can be used for the research of cancer, such as colon carcinoma.
For research use only. We do not sell to patients.
- Purity: 99.34%
- CAS No.: 3050832-41-9
- Formula: C28H32N6O8
- Molecular Weight:580.59
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Tubulin/CDC5L-IN-1 (Compound 11i) (48 h) inhibits HeLa, HCT 116, A2780, and A549 cells proliferation with IC50 values of 0.046, 0.035, 0.096 and 0.112 μM[1].
Tubulin/CDC5L-IN-1 shows antiproliferative efficacy in drug-resistant cancer cells with IC50 ranging from 0.089-0.172 μM[1].
Tubulin/CDC5L-IN-1 (1-100 μM, 0-30 mins) inhibits the microtubule assembly with an IC50 of 28.8 μM[1].
Tubulin/CDC5L-IN-1 (0.125-0.05 μM, 24 h) inhibits colony formation in HeLa and HCT 116 cells[1].
Tubulin/CDC5L-IN-1 (0.125-0.1 μM, 12 h) induces G2/M phase arrest in HCT 116 cells[1].
Tubulin/CDC5L-IN-1 (0.05-0.2 μM, 24 h) induces apoptosis and ROS production in HCT 116 cells[1].
Tubulin/CDC5L-IN-1 (0.05-0.2 μM, 4 h) inhibits capillary tube-like formation in HUVECs[1].
Tubulin/CDC5L-IN-1 (5 μM) inhibits DNA-dependent protein kinase and cell division cycle 7[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT 116 cells
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Concentration:0.125, 0.05 and 0.1 μM
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Incubation Time:12 h
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Result:Increased cells in the G2/M phase.
Increased the phosphorylation level of histone H3 (PHH3) at Ser10.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:HCT116 xenograft mice models[1]
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Dosage:7.5, 10 and 20 mg/kg
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Administration:Intraperitoneally injection, every 2 days
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Result:Reduced tumor weight and volume.
Had no body weight loss.
Decreased Ki67-positive cells and reduced tumor microvessel density.
Had no histopathological changes in the heart, liver, spleen, lungs, or kidneys.
Chemical Information
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CAS No. 3050832-41-9
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Appearance Solid
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Molecular Weight 580.59
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Formula C28H32N6O8
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Color White to off-white
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SMILES
CC(C)C[C@H](C(O)=O)NC(OC1=CC(C2=NC(N)=C3C(N(C4=CC(OC)=C(OC)C(OC)=C4)N=C3)=N2)=CC=C1OC)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (172.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.31 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7224 mL | 8.6119 mL | 17.2239 mL | 43.0596 mL |
| 5 mM | 0.3445 mL | 1.7224 mL | 3.4448 mL | 8.6119 mL | |
| 10 mM | 0.1722 mL | 0.8612 mL | 1.7224 mL | 4.3060 mL | |
| 15 mM | 0.1148 mL | 0.5741 mL | 1.1483 mL | 2.8706 mL | |
| 20 mM | 0.0861 mL | 0.4306 mL | 0.8612 mL | 2.1530 mL | |
| 25 mM | 0.0689 mL | 0.3445 mL | 0.6890 mL | 1.7224 mL | |
| 30 mM | 0.0574 mL | 0.2871 mL | 0.5741 mL | 1.4353 mL | |
| 40 mM | 0.0431 mL | 0.2153 mL | 0.4306 mL | 1.0765 mL | |
| 50 mM | 0.0344 mL | 0.1722 mL | 0.3445 mL | 0.8612 mL | |
| 60 mM | 0.0287 mL | 0.1435 mL | 0.2871 mL | 0.7177 mL | |
| 80 mM | 0.0215 mL | 0.1076 mL | 0.2153 mL | 0.5382 mL | |
| 100 mM | 0.0172 mL | 0.0861 mL | 0.1722 mL | 0.4306 mL |
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