2. Cell Cycle/DNA Damage Cytoskeleton
  3. Microtubule/Tubulin
  4. Tubulin-IN-52

Tubulin-IN-52 is a tubulin inhibitor. Tubulin-IN-52 demonstrates significant cytotoxicity toward a variety of cancer cell types including prostate, lung, and ovarian cancers with strong activity toward highly aggressive cancer lines (IC50 = 0.9-3.8 mM). Tubulin-IN-52 significantly inhibits the growth of established tumors without noticeable toxicity. Tubulin-IN-52 can be used for the study of cancer.

For research use only. We do not sell to patients.

Tubulin-IN-52

Tubulin-IN-52 Chemical Structure

CAS No. : 2099064-75-0

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Tubulin-IN-52 Related Antibodies

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Description

Tubulin-IN-52 is a tubulin inhibitor. Tubulin-IN-52 demonstrates significant cytotoxicity toward a variety of cancer cell types including prostate, lung, and ovarian cancers with strong activity toward highly aggressive cancer lines (IC50 = 0.9-3.8 mM). Tubulin-IN-52 significantly inhibits the growth of established tumors without noticeable toxicity. Tubulin-IN-52 can be used for the study of cancer[1].

Cellular Effect
Cell Line Type Value Description References
A549 EC50
1.4 μM
Compound: 18
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
A549/TR EC50
3.8 μM
Compound: 18
Cytotoxicity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human A549/TR cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
Calu-1 EC50
1.6 μM
Compound: 18
Cytotoxicity against human Calu1 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human Calu1 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
NCI-H157 EC50
2.4 μM
Compound: 18
Cytotoxicity against human NCI-H157 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human NCI-H157 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
PC-3 EC50
1.6 μM
Compound: 18
Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
SK-OV-3 EC50
1 μM
Compound: 18
Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 72 hrs by MTT assay
[PMID: 29288939]
In Vitro

Tubulin-IN-52 (Compound 18) (1-10 μM, 72 h) induces significant cytotoxicity in the cell line A549, and the prostate cancer cell lines PC3 and PC3MLN4[1].
Tubulin-IN-52 (0.0032-50 μM) shows highly cytotoxic in cancer cells with EC50s of 2.4 μM (H157), 1.6 μM (Calu1), 1.4 μM (A549), 1.6 μM (PC3), 0.9 μM (PC3MLN4) and 1.0 μM (SKOV3)[1].
Tubulin-IN-52 (1 μM, 24 h) induces G2M cell cycle arrest in PC3MLN4 cells[1].
Tubulin-IN-52 (1 μM, 48 h) disrupts microtubule dynamics in PC3MLN4 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Tubulin-IN-52 Related Antibodies

Cell Viability Assay[1]

Cell Line: PC3MLN4, PC3 and A549 cells
Concentration: 1, 10 μM
Incubation Time: 72 h
Result: Induced significant cytotoxicity at 1 and 10 μM.

Cell Cycle Analysis[1]

Cell Line: PC3MLN4 cells
Concentration: 1 μM
Incubation Time: 24 h
Result: Induced G2M cell cycle arrest in PC3MLN4 cells.

Western Blot Analysis[1]

Cell Line: PC3MLN4, PC3 and A549 cells
Concentration: 1 μM
Incubation Time: 48 h
Result: Increased the level of cyclin B1.
In Vivo

Tubulin-IN-52 (Compound 18) (30 mg/kg, i.p., 3 times a week, two weeks ) shows significant inhibition of tumor growth in the PC3MLN4 xenograft mouse model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: PC3MLN4 cells (106)-derived subcutaneous xenograft athymic male mice (eight weeks)[1]
Dosage: 30 mg/kg
Administration: i.p. 3 times a week for two weeks
Result: Showed tumor volume (day 15) of 43.2 mm3 while that of the control group (C) is 118.8 mm3.
Showed treatment-to-control (T/C) ratio at the last time point of 0.36, below the T/C 0.45 threshold that defines active antitumor activity.
Showed no noticeable toxicity.
Molecular Weight

380.40

Formula

C20H20N4O4
CAS No.
SMILES

O=C(NC1=NC2=C(N1)C=C(C(C3=CC=C(N(C4COC4)C)C=C3)=O)C=C2)OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Tubulin-IN-52 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Tubulin-IN-522099064-75-0Microtubule/TubulintubulincancerPC3MLN4 cellsPC3 cellsA549 cellsInhibitorinhibitorinhibit

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