Tubulin inhibitor 30
Based on 1 Customer Validation
Tubulin inhibitor 30 (Compound 15) is a tubulin assembly inhibitor with an IC50 of 0.52 μM. Tubulin inhibitor 30 can induce ferroptosis.
For research use only. We do not sell to patients.
- Purity: 99.77%
- CAS No.: 2873383-67-4
- Formula: C22H19N3O5
- Molecular Weight:405.40
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
IC50: 0.52 μM (Tubulin)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | IC50 |
4 nM
Compound: 15
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Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by trypan blue exclusion assay
Cytotoxicity against human MCF7 cells assessed as inhibition of cell growth incubated for 96 hrs by trypan blue exclusion assay
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[PMID: 36395526] |
| OVCAR-3 | IC50 |
2.85 nM
Compound: 15
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Cytotoxicity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay
Cytotoxicity against human OVCAR-3 cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay
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[PMID: 36395526] |
| U-87MG ATCC | IC50 |
10.06 nM
Compound: 15
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Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay
Cytotoxicity against human U-87 MG cells assessed as inhibition of cell growth incubated for 48 hrs by trypan blue exclusion assay
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[PMID: 36395526] |
Tubulin inhibitor 30 (Compound 15) (0-100 nM; 48 h) inhibits the viability of MCF-7, U-87 MG and OVCAR-3 cells[1].
Tubulin inhibitor 30 is metabolically stable when incubated with human liver microsomes[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7, U-87 MG and OVCAR-3 cells
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Concentration:0-100 nM
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Incubation Time:48 h
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Result:Inhibited the cell viability with IC50s of 4.0, 10.06 and 2.852 nM against MCF-7, U-87 MG and OVCAR-3 cells, respectively.
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Cell Line:U-87 MG and OVCAR-3 cells
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Concentration:0-100 nM
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Incubation Time:48 h
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Result:Induced a significant increase of cleaved PARP.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/Cnu/nu mice, U-87 MG or SKOV-3 model[1]
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Dosage:25 mg/kg
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Administration:Intraperitoneal injection, every 2 days
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Result:Inhibited tumorigenicity and tumor volume. Caused oxidative stress injury and Fe2+ accumulation.
Chemical Information
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CAS No. 2873383-67-4
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Appearance Solid
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Molecular Weight 405.40
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Formula C22H19N3O5
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Color White to off-white
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SMILES
COC1=CC(C(C2=CN(C=C2C3=CC=CO3)C4=NC=CC=N4)=O)=CC(OC)=C1OC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 5 mg/mL (12.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4667 mL | 12.3335 mL | 24.6670 mL | 61.6675 mL |
| 5 mM | 0.4933 mL | 2.4667 mL | 4.9334 mL | 12.3335 mL | |
| 10 mM | 0.2467 mL | 1.2333 mL | 2.4667 mL | 6.1667 mL |
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