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Cat. No.: HY-146818

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Tubulin polymerization-IN-12

Tubulin polymerization-IN-12 is a tubulin polymerization inhibitor (IC₅₀=0.75 μM). Tubulin polymerization-IN-12 arrests cell cycle at G2/M phase, and exhibits cytotoxicity against cancer cells.

For research use only. We do not sell to patients.

CAS No.: 2377301-45-4
Formula: C₂₃H₂₃N₅O₆
Molecular Weight:465.46

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (278 KB)

Tubulin polymerization-IN-12 Chemical Structure

CAS No.: 2377301-45-4

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	1.02 μM Compound: 26	Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells incubated for 48 hrs by MTT assay	[PMID: 39285177]
HEK293	IC₅₀	29.94 μM Compound: 6	Cytotoxicity against HEK293 cells measured after 48 hrs by MTT assay Cytotoxicity against HEK293 cells measured after 48 hrs by MTT assay	[PMID: 32717486]
HeLa	IC₅₀	0.75 μM Compound: 6	Antiproliferative activity against human HeLa cells measured after 48 hrs by MTT assay Antiproliferative activity against human HeLa cells measured after 48 hrs by MTT assay	[PMID: 32717486]
HeLa	IC₅₀	0.75 μM Compound: 26	Antiproliferative activity against human HeLa cells incubated for 48 hrs by MTT assay Antiproliferative activity against human HeLa cells incubated for 48 hrs by MTT assay	[PMID: 39285177]

In Vitro

Tubulin polymerization-IN-12 (compound 6) shows cytotoxicity against normal human embryonic kidney cells, HEK-293, with an IC₅₀ value of 29.94 μM^[1].
Tubulin polymerization-IN-12 (compound 26) inhibits cancer cells with IC₅₀s of 1.02 μM (A549), 0.75 μM (HeLa), 10.91 μM (HCT116), and 29.94 μM (HEK293), respectively^[2].
Tubulin polymerization-IN-12 (1 μM, 2 μM, 10 μM; 6 h) inhibits tubulin expression in HeLa cell, and shows 42% inhibition at 10 μM^[2].
Tubulin polymerization-IN-12 (0.75 μM-3 μM; 24 h) arrests cell cycle at G2/M phase in HeLa cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Immunofluorescence^[2]

Cell Line:

HeLa cells
Concentration:

1 μM, 2 μM
Incubation Time:

6 hours
Result:

Dose-dependently decreased the level of α and β tubulin.

Cell Cycle Analysis^[2]

Cell Line:

HeLa cells
Concentration:

0.75 μM, 1.5 μM, 3 μM
Incubation Time:

24 hours
Result:

Demonstrated 15.03% (0.75 μM), 20.04% (1.50 μM), and 33.47% (3.00 μM) of cell accumulation in G2/M phase.

Chemical Information

CAS No. 2377301-45-4
Molecular Weight 465.46
Formula C₂₃H₂₃N₅O₆
SMILES

COC1=CC(C2=NC3=NC(C)=C(C(N3N2)C4=CC=C(C=C4)[N+]([O-])=O)C(C)=O)=CC(OC)=C1OC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Yang F, et al. Synthesis, and biological evaluation of 3, 6-diaryl-[1, 2, 4] triazolo [4, 3-a] pyridine analogues as new potent tubulin polymerization inhibitors[J]. European Journal of Medicinal Chemistry, 2020, 204: 112625.

[2]. Yang F, et al. Novel [1, 2, 4] triazolo [1, 5-a] pyrimidine derivatives as potent antitubulin agents: Design, multicomponent synthesis and antiproliferative activities[J]. Bioorganic Chemistry, 2019, 92: 103260.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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