country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Cat. No.: HY-101989

Copy Link Print Information

Tubulin polymerization-IN-24

Tubulin polymerization-IN-24 (compound HMBA) is a potent tubulin polymerization inhibitor. Tubulin polymerization-IN-24 inhibits MCF-7 cells proliferation. Tubulin polymerization-IN-24 induces apoptosis and cell cycle arrest at G2/M phase. Tubulin polymerization-IN-24 increase the GTP hydrolysis rate and inhibits microtubule assembly.

For research use only. We do not sell to patients.

CAS No.: 564468-51-5
Formula: C₂₂H₁₆O₃
Molecular Weight:328.36

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (272 KB)

Tubulin polymerization-IN-24 Chemical Structure

CAS No.: 564468-51-5

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
K562	EC₅₀	0.206 μM Compound: 9h	Effective concentration required to arrest propidium iodide stained K562 cells in G2/M phase Effective concentration required to arrest propidium iodide stained K562 cells in G2/M phase	[PMID: 12852768]
K562	IC₅₀	0.02 μM Compound: 8	Antiproliferative activity against human K562 cells after 48 hrs by hemocytometer Antiproliferative activity against human K562 cells after 48 hrs by hemocytometer	[PMID: 21705223]
K562	IC₅₀	0.02 μM Compound: 9h	In vitro inhibitory concentration against human chronic myelogenous leukemia K562 cell growth In vitro inhibitory concentration against human chronic myelogenous leukemia K562 cell growth	[PMID: 12852768]
KB	EC₅₀	0.2 μM Compound: 10	Cell cycle arrest in KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs by flow cytometric analysis Cell cycle arrest in KB/HeLa cells assessed as accumulation at G2/M phase after 24 hrs by flow cytometric analysis	[PMID: 17973361]
KB	IC₅₀	0.09 μM Compound: 9h	Cytotoxic activity against cellular metabolic activity of Cervix KB/HeLa tumor cell line using XTT proliferation assay after incubation with the compound for 48 h Cytotoxic activity against cellular metabolic activity of Cervix KB/HeLa tumor cell line using XTT proliferation assay after incubation with the compound for 48 h	[PMID: 12852768]
L1210	IC₅₀	0.13 μM Compound: 9h	Antiproliferative activity against L1210 phenotype L1210 VCR tumor cell line Antiproliferative activity against L1210 phenotype L1210 VCR tumor cell line	[PMID: 12852768]
L1210	IC₅₀	0.14 μM Compound: 9h	Antiproliferative activity against L1210 tumor cell line Antiproliferative activity against L1210 tumor cell line	[PMID: 12852768]
LT12 tumor cell line	IC₅₀	0.19 μM Compound: 9h	Antiproliferative activity against LT12 phenotype LT12 MDR tumor cell line Antiproliferative activity against LT12 phenotype LT12 MDR tumor cell line	[PMID: 12852768]
LT12 tumor cell line	IC₅₀	0.32 μM Compound: 9h	Antiproliferative activity against LT12 tumor cell line Antiproliferative activity against LT12 tumor cell line	[PMID: 12852768]
Lung LXF 629L tumor cell line	IC₅₀	<0.01 μM Compound: 9h	Tested for induction of cellular death in Human lung LXF 629L tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human lung LXF 629L tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
LXFL 529	IC₅₀	0.52 μM Compound: 9h	Tested for induction of cellular death in Human lung LXF 529L tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human lung LXF 529L tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
MAXF401 cell line	IC₅₀	<0.01 μM Compound: 9h	Tested for induction of cellular death in Human breast 401NL tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human breast 401NL tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
MCF7	IC₅₀	<0.01 μM Compound: 9h	Tested for induction of cellular death in Human breast MCF-7 tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human breast MCF-7 tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
NCI-H460	IC₅₀	<0.01 μM Compound: 9h	Tested for induction of cellular death in Human lung H-460 tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human lung H-460 tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
NCI-H460	IC₅₀	0.07 μM Compound: 9h	Cytotoxic activity against cellular metabolic activity of Lung NCI-H460 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h Cytotoxic activity against cellular metabolic activity of Lung NCI-H460 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h	[PMID: 12852768]
P388/ADR	IC₅₀	0.16 μM Compound: 9h	Antiproliferative activity against P388 tumor cell line Antiproliferative activity against P388 tumor cell line	[PMID: 12852768]
P388/ADR	IC₅₀	0.26 μM Compound: 9h	Antiproliferative activity against P388 phenotype P388 ADR tumor cell line Antiproliferative activity against P388 phenotype P388 ADR tumor cell line	[PMID: 12852768]
RKO	IC₅₀	>9 μM Compound: 9h	Cytotoxic activity against human colon adenocarcinoma RKO cells with ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1 Cytotoxic activity against human colon adenocarcinoma RKO cells with ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1	[PMID: 12852768]
RKO	IC₅₀	0.06 μM Compound: 9h	Cytotoxic activity against human colon adenocarcinoma RKO cells without ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1 Cytotoxic activity against human colon adenocarcinoma RKO cells without ectopic inducible expression of cyclin-dependent kinase inhibitor p27kip1	[PMID: 12852768]
RXF 944	IC₅₀	<0.01 μM Compound: 9h	Tested for induction of cellular death in Human renal RXF 944L tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human renal RXF 944L tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
SF-268	IC₅₀	0.03 μM Compound: 9h	Tested for induction of cellular death in Human ZNS SF-268 tumor cell line in Propidium Iodide Monolayer Assay Tested for induction of cellular death in Human ZNS SF-268 tumor cell line in Propidium Iodide Monolayer Assay	[PMID: 12852768]
SF-268	IC₅₀	0.05 μM Compound: 9h	Cytotoxic activity against cellular metabolic activity of Glioma SF-268 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h Cytotoxic activity against cellular metabolic activity of Glioma SF-268 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h	[PMID: 12852768]
SK-OV-3	IC₅₀	0.05 μM Compound: 9h	Cytotoxic activity against cellular metabolic activity of Ovary SKOV3 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h Cytotoxic activity against cellular metabolic activity of Ovary SKOV3 tumor cell line using XTT proliferation assay after incubation with the compound for 48 h	[PMID: 12852768]

In Vitro

Tubulin polymerization-IN-24 (compound HMBA) inhibits the assembly of purified tubulin (MAPs-free) in a concentration dependent manner^[1].
Tubulin polymerization-IN-24 (0-100 nM) inhibits MCF-7 proliferation in a concentration dependent fashion with an IC₅₀ value of 47 nM^[1].
Tubulin polymerization-IN-24 competitively inhibits colchicine binding to tubulin with a K_i value of 3.6 μM^[1].
Tubulin polymerization-IN-24 inhibits the progression of MCF-7 cells in mitosis and induces apoptotic cell death involving p53 pathway^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 564468-51-5
Molecular Weight 328.36
Formula C₂₂H₁₆O₃
SMILES

O=C1C2=C(C=CC=C2)/C(C3=CC=CC=C13)=C\C4=CC=C(OC)C(O)=C4
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chatterji BP, et al. HMBA depolymerizes microtubules, activates mitotic checkpoints and induces mitotic block in MCF-7 cells by binding at the colchicine site in tubulin. Biochem Pharmacol. 2010 Jul 1;80(1):50-61. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

Minimum order quantity

100 mg

Get Quote In-stock

Other size

Get Quote

Amount: USD 0.00

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Tubulin polymerization-IN-24

High-Throughput Bioactive Compound Library

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Tubulin polymerization-IN-24 Related Classifications

HY-101989 Related Classifications