Tubulin polymerization-IN-56
Based on 1 Customer Validation
Tubulin polymerization-IN-56 (compound 8l), an indazole derivative, is a potent tubulin polymerization inhibitor through interacting with the colchicine site, resulting in cell cycle arrest and cellular apoptosis. polymerization-IN-56 reduces cell migration and leads to more potent inhibition of tumor growth in vivo.
For research use only. We do not sell to patients.
- Purity: 99.96%
- CAS No.: 2966790-98-5
- Formula: C22H22ClN3O3
- Molecular Weight:411.88
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Bioactive Compound Library Max
HY-L181
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Bioactive Compound Library
HY-L001
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Apoptosis Compound Library
HY-L003
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Cell Cycle/DNA Damage Compound Library
HY-L004
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Anti-Cancer Compound Library
HY-L025
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Anti-Aging Compound Library
HY-L034
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Cytoskeleton Compound Library
HY-L060
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Anti-Lung Cancer Compound Library
HY-L075
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Protein-protein Interaction Inhibitor Library
HY-L109
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Cell Death Library
HY-L162
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MCE Bioactive Compound Library
HY-L001V
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
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High-Efficiency Gene Editing Compound Library
HY-L244
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.027 μM
Compound: 8l
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Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
Antiproliferative activity against human A549 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
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[PMID: 37890199] |
| A549/TR | IC50 |
33 nM
Compound: 8l
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Antiproliferative activity against human A549/Taxol cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
Antiproliferative activity against human A549/Taxol cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
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[PMID: 37890199] |
| Huh-7 | IC50 |
0.03 μM
Compound: 8l
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Antiproliferative activity against human Huh-7 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
Antiproliferative activity against human Huh-7 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
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[PMID: 37890199] |
| T-24 | IC50 |
0.046 μM
Compound: 8l
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Antiproliferative activity against human T-24 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
Antiproliferative activity against human T-24 cells assessed as cell growth inhibition incubated for72 hrs by MTT assay
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[PMID: 37890199] |
Chemical Information
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CAS No. 2966790-98-5
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Appearance Solid
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Molecular Weight 411.88
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Formula C22H22ClN3O3
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Color Light yellow to yellow
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SMILES
CC1=CC=C(C2=CC=C3C(N(C4=CC(OC)=C(OC)C(OC)=C4)N=C3)=C2)C=N1.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 40 mg/mL (97.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 4 mg/mL (9.71 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 4 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 4 mg/mL (9.71 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 4 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (40.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (275 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4279 mL | 12.1395 mL | 24.2789 mL | 60.6973 mL |
| 5 mM | 0.4856 mL | 2.4279 mL | 4.8558 mL | 12.1395 mL | |
| 10 mM | 0.2428 mL | 1.2139 mL | 2.4279 mL | 6.0697 mL | |
| 15 mM | 0.1619 mL | 0.8093 mL | 1.6186 mL | 4.0465 mL | |
| 20 mM | 0.1214 mL | 0.6070 mL | 1.2139 mL | 3.0349 mL | |
| 25 mM | 0.0971 mL | 0.4856 mL | 0.9712 mL | 2.4279 mL | |
| 30 mM | 0.0809 mL | 0.4046 mL | 0.8093 mL | 2.0232 mL | |
| 40 mM | 0.0607 mL | 0.3035 mL | 0.6070 mL | 1.5174 mL | |
| 50 mM | 0.0486 mL | 0.2428 mL | 0.4856 mL | 1.2139 mL | |
| 60 mM | 0.0405 mL | 0.2023 mL | 0.4046 mL | 1.0116 mL | |
| 80 mM | 0.0303 mL | 0.1517 mL | 0.3035 mL | 0.7587 mL |
Tubulin polymerization-IN-56 Related Classifications
HY-155362 Related Classifications
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