Cat. No.: HY-15477A

YS-49 monohydrate

15 Cited Publications

Customer Review

Based on 15 publication(s) in Google Scholar

YS-49 (monohydrate) is a PI3K/Akt (a downstream target of RhoA) activator, to reduce RhoA/PTEN activation in the 3-methylcholanthrene-treated cells. YS-49 inhibits angiotensin II (Ang II)-stimulated proliferation of VSMCs via induction of heme oxygenase (HO)-1. YS-49 is also an isoquinoline compound alkaloid, has a strong positive inotropic action through activation of cardiac β-adrenoceptors.

For research use only. We do not sell to patients.

Purity: 99.56%
CAS No.: 3028631-24-2
Formula: C₂₀H₂₂BrNO₃
Molecular Weight:404.30

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

YS-49 monohydrate Chemical Structure

CAS No.: 3028631-24-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (15)
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

PI3K/Akt^[3]

All Akt Isoforms :

All PI3K Isoforms :

All Angiotensin Receptor Isoforms :

All Adrenergic Receptor Isoforms :

In Vitro

YS-49 (1-100?μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC₅₀ values of 22 μM and 30?μM, respectively^[2].
YS-49 (10-100?μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

RAVSMC and RAW 264.7 cells
Concentration:

10 μΜ, 30 μΜ and 100 μΜ(RAVSMC); 1 μΜ, 10 μΜ and 100 μM (RAW 264.7)
Incubation Time:

18 hours
Result:

Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC₅₀ values of 22 and 30 μM, respectively.

Western Blot Analysis^[2]

Cell Line:

RAVSMC and RAW 264.7 cells
Concentration:

10 μΜ, 30 μΜ and 100 μΜ
Incubation Time:

18 hours
Result:

Concentration-dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ.

In Vivo

YS-49 (5?mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague Dawley rats (250-300 g)^[2]
Dosage:

5 mg/kg
Administration:

Intraperitoneal injection; 8 hours
Result:

Serum NOx levels were significantly reduced.

Chemical Information

CAS No. 3028631-24-2
Appearance Solid
Molecular Weight 404.30
Formula C₂₀H₂₂BrNO₃
Color Off-white to gray
SMILES

OC1=CC2=C(C(CC3=C4C=CC=CC4=CC=C3)NCC2)C=C1O.O.Br
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (15)

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Signal Transduct Target Ther

M2 macrophages, but not M1 macrophages, support megakaryopoiesis by upregulating PI3K-AKT pathway activity. [Abstract]

2021 Jun 18;6(1):234. PMID: 34140465
Sci China Life Sci

Integrating spatial transcriptomics and single-nucleus RNA-seq revealed the specific inhibitory effects of TGF-β on intramuscular fat deposition. [Abstract]

2025 Mar;68(3):746-763. PMID: 39422812
Front Immunol

"γδT Cell-IL17A-Neutrophil" Axis Drives Immunosuppression and Confers Breast Cancer Resistance to High-Dose Anti-VEGFR2 Therapy. [Abstract]

2021 Oct 15;12:699478. PMID: 34721375
Biochem Pharmacol

Columbianadin suppresses glioblastoma progression by inhibiting the PI3K-Akt signaling pathway. [Abstract]

2024 May:223:116112. PMID: 38458331
Mol Ther Oncolytics

PI3K-activated MSC proteomes inhibit mammary tumors via Hsp90ab1 and Myh9. [Abstract]

2022 Aug 5:26:360-371. PMID: 36090473
Commun Biol

DTX1-mediated degradation of TUBB3 in Kupffer cells mitigates hepatocellular carcinoma progression by regulating M1/M2 polarization. [Abstract]

2026 Jan 23;9(1):311. PMID: 41577987
Pharm Biol

Chinese herbal compound Huangqin Qingrechubi capsule reduces lipid metabolism disorder and inflammatory response in gouty arthritis via the LncRNA H19/APN/PI3K/AKT cascade. [Abstract]

2023 Dec;61(1):541-555. PMID: 36994890
Cancers (Basel)

Generation of the Chondroprotective Proteomes by Activating PI3K and TNFα Signaling. [Abstract]

2022 Jun 21;14(13):3039. PMID: 35804814
Sci Rep

The inhibition of pancreatic cancer progression by K-Ras-overexpressing mesenchymal stem cell-derived secretomes. [Abstract]

2023 Sep 12;13(1):15036. PMID: 37699930
Mol Med Rep

Mechanism of β‑sitosterol in treating keloids: Network pharmacology, molecular docking and experimental verification. [Abstract]

2025 Apr;31(4):95. PMID: 39981895
J Anim Sci

Roles of JNK, ERK, and PI3K/AKT signaling pathways in zinc-mediated alleviation of thermal stress-induced damage to the integrity and barrier function of primary cultured chick jejunal epithelial cells. [Abstract]

2025 Jan 4:103:skaf266. PMID: 40795135
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
BMC Musculoskelet Disord

YS-49 activates the PI3K/AKT signaling pathway in MC3T3-E1 cells to enhance osteoblast differentiation and inhibits glucocorticoid-induced bone loss in vivo. [Abstract]

2025 Oct 14;26(1):961. PMID: 41088103
Int J Rheum Dis

2024 Jun 22.
SSRN

2026 Mar 10.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Sun JJ, et al. YS 49, 1-(alpha-naphtylmethyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, regulates angiotensin II-stimulated ROS production, JNK phosphorylation and vascular smooth muscle cell proliferation via the induction of heme oxygenase-1. Life Sci. 2008 Mar 12;82(11-12):600-7. [Content Brief]

[2]. Kang YJ, et al. Prevention of the expression of inducible nitric oxide synthase by a novel positive inotropic agent, YS 49, in rat vascular smooth muscle and RAW 264.7 macrophages. Br J Pharmacol. 1999 Sep;128(2):357-64. [Content Brief]

[3]. Hsu YH, et al. RhoA-mediated inhibition of vascular endothelial cell mobility: positive feedback through reduced cytosolic p21 and p27. J Cell Physiol. 2014 Oct;229(10):1455-65. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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YS-49 monohydrate

15 Cited Publications

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YS-49 monohydrate

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Publications Citing Use of MedChemExpress (MCE) YS-49 monohydrate

All Akt Isoforms

All PI3K Isoforms

All Angiotensin Receptor Isoforms

All Adrenergic Receptor Isoforms

Biological Activity

Chemical Information

Publications (15)

Purity & Documentation

References

Calculators

Help & FAQs

YS-49 monohydrate Related Classifications

Cat. No.: HY-15477A