1. PI3K/Akt/mTOR
    GPCR/G Protein
    Neuronal Signaling
  2. Akt
    PI3K
    Angiotensin Receptor
    Adrenergic Receptor
  3. YS-49 monohydrate

YS-49 monohydrate 

Cat. No.: HY-15477A Purity: >98.0%
Handling Instructions

YS-49 (monohydrate) is a PI3K/Akt (a downstream target of RhoA) activator, to reduce RhoA/PTEN activation in the 3-methylcholanthrene-treated cells. YS-49 inhibits angiotensin II (Ang II)-stimulated proliferation of VSMCs via induction of heme oxygenase (HO)-1. YS-49 is also an isoquinoline compound alkaloid, has a strong positive inotropic action through activation of cardiac β-adrenoceptors.

For research use only. We do not sell to patients.

YS-49 monohydrate Chemical Structure

YS-49 monohydrate Chemical Structure

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 174 In-stock
Estimated Time of Arrival: December 31
10 mg USD 158 Get quote
50 mg USD 557 Get quote
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Description

YS-49 (monohydrate) is a PI3K/Akt (a downstream target of RhoA) activator, to reduce RhoA/PTEN activation in the 3-methylcholanthrene-treated cells. YS-49 inhibits angiotensin II (Ang II)-stimulated proliferation of VSMCs via induction of heme oxygenase (HO)-1. YS-49 is also an isoquinoline compound alkaloid, has a strong positive inotropic action through activation of cardiac β-adrenoceptors[1][2][3].

IC50 & Target

PI3K/Akt[3]

In Vitro

YS-49 (1-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) concentration-dependently inhibits the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to lipopolysaccharide (LPS) plus INF-γ, with IC50 values of 22 μM and 30 μM, respectively[2].
YS-49 (10-100 μM; 18 hours; RAVSMC and RAW 264.7 cells) suppresses iNOS gene expression induced by LPS and/or cytokines in RAVSMC and RAW 264.7 cells at the transcriptional level[2].

Cell Viability Assay[2]

Cell Line: RAVSMC and RAW 264.7 cells
Concentration: 10 μΜ, 30 μΜ and 100 μΜ(RAVSMC); 1 μΜ, 10 μΜ and 100 μM (RAW 264.7)
Incubation Time: 18 hours
Result: Inhibited the accumulation of nitrite in both RAVSMC and RAW 264.7 exposed to LPS+INF-γ, with IC50 values of 22 and 30 μM, respectively.

Western Blot Analysis[2]

Cell Line: RAVSMC and RAW 264.7 cells
Concentration: 10 μΜ, 30 μΜ and 100 μΜ
Incubation Time: 18 hours
Result: Concentration-dependently inhibited the expression of iNOS protein induced by LPS plus IFN-γ.
In Vivo

YS-49 (5 mg/kg; intraperitoneal injection; 8 hours; male Sprague Dawley rats) treatment significantly reduces serum NOx levels in LPS-treated rats, the NOx levels reduce from 86 μM to 34 μM[2].

Animal Model: Male Sprague Dawley rats (250-300 g)[2]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; 8 hours
Result: Serum NOx levels were significantly reduced.
Molecular Weight

404.30

Formula

C₂₀H₂₂BrNO₃

SMILES

OC1=CC2=C(C(CC3=C4C=CC=CC4=CC=C3)NCC2)C=C1O.O.Br

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
References

Purity: >98.0%

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Keywords:

YS-49YS49YS 49AktPI3KAngiotensin ReceptorAdrenergic ReceptorPKBProtein kinase BPhosphoinositide 3-kinaseBeta ReceptorInhibitorinhibitorinhibit

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Product Name:
YS-49 monohydrate
Cat. No.:
HY-15477A
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